Plant Expression System - PowerPoint PPT Presentation

Slide1 l.jpg
1 / 12

  • Updated On :
  • Presentation posted in: General

Plant Expression System. M olecular B iology Lab oratory Oh Chang Jae. Overview of methods for plant transformation. A. Delivery of DNA into a single plant cell → transient assay B. Integration of DNA into the plant cell genome

Related searches for Plant Expression System

I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.

Download Presentation

Plant Expression System

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.

- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript

Slide1 l.jpg

Plant Expression System

Molecular Biology Laboratory

Oh Chang Jae

Slide2 l.jpg

Overview of methods for plant transformation

A. Delivery of DNA into a single plant cell→ transient assay

B. Integration of DNA into the plant cell genome

C. Regenerationof the transformed cell into a whole plant

Slide3 l.jpg

In planta transformation with Agrobacterium

Vacuum infiltration

(Bechtold et al. 1993 )

Floral dipping method

(Bent et al., 1998)


with Agrobacterium

Slide4 l.jpg

Agrobacterium tumefaciens

- crown gall disease

- Ti plasmid : T-DNA + vir genes

( > 200 Kbp )

Slide5 l.jpg

Host Range

… cause disease primarily on dicots.

However, they can even infect gymnosperms,

not infectious on monocots, although transfer of the T-DNA into monocot can occur.


1. Tumor formation caused by the elevation of auxin and cytokinin levels by genes on a natural T-DNA

2. Opines synthesis

T-DNA used for scientific experimentation have usually been deleted of iaaM, iaaH, ipt and opine synthesis genes. → “ disarmed”

Slide6 l.jpg

T-DNA transfer as mediated by vir gene functions

Slide7 l.jpg

Plant transformation vector

Binary vector system

- Basic cloning vector (plant expression vector)

- plant transformation vector

Cointegrate vector system

- Cointegrate vector

- Disarmed Ti plasmid

Slide8 l.jpg

General Vector composition

√T-DNA cassette

: Ti-plasmid right border (RB) + MCS + positive selectable marker + Ti-plasmid left border (LB)


Constitutve : 35S CaMV (Cauliflower mosaic virus)

Inducible : heat shock, glucocorticoid hormones, alcohol etc.

A plant promoter will often work in many different plant species, but yeast or human or bacterial promoter do not function.


Derived from CaMV, nopaline synthase gene etc.

√Common plant selectable marker : antibiotics , herbicides

Slide9 l.jpg

Schematic diagram of binary vector

Slide10 l.jpg

Basic cloning vector (pRT101)

P35S : CaMV 35S Promoter

pACaMV :CaMV polyadenylation site

ColE1 replication origin

bla ; Amp resistance gene

Slide12 l.jpg

Plant transformation vector (pBIN20)

Kanamycine Resistance

NOS promoter

NOS terminator


  • Login