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PCR 技术介绍 Introduction to PCR Technology PowerPoint PPT Presentation


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PCR 技术介绍 Introduction to PCR Technology. 济南艾迪康医学检验中心有限公司 PCR 实验室 杨超. 主要内容. 一. DNA 结构和复制 二. PCR 1. PCR 发展简史 2. PCR 基本概念 3. PCR 原理 三. 荧光定量 PCR 检测 HBV 1. 荧光定量 PCR 原理 2. 荧光定量 PCR 工作流程 3. 荧光定量 PCR 结果分析 4. 荧光定量 PCR 应用 四.基因芯片技术平台

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PCR 技术介绍 Introduction to PCR Technology

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Pcr introduction to pcr technology

PCRIntroduction to PCR Technology

PCR


Pcr introduction to pcr technology

. DNA

. PCR

1. PCR

2. PCR

3. PCR

. PCRHBV

1. PCR 2. PCR

3. PCR 4. PCR

.

HPV23


Pcr introduction to pcr technology

DNA

DNA


Pcr introduction to pcr technology

O-

O-

5

5

O=P

O=P

OCH2

OCH2

C

T

O

O

O-

O-

3

3

OH

OH

H

H

O

5

O=P

OCH2

C

O

O-

3

OH

H

A

G

C

T


Pcr introduction to pcr technology

DNA

DNA


Pcr introduction to pcr technology

1.DNA

2.RNA


Pcr introduction to pcr technology

3.DNA


Pcr introduction to pcr technology

  • 1985PE-CetusMullisPCR

  • DNA

  • E-coli DNAPCR

  • DNAPCRPE-CetusPCR

  • 1993Mullis


Pcr introduction to pcr technology

PCR

(Polymerase Chain Reaction)


Pcr introduction to pcr technology

PCR

DNA Mg2+


Pcr introduction to pcr technology

DNA


Pcr introduction to pcr technology

95

55

72

Taq

2

1

Taq


Pcr introduction to pcr technology

1

2


Pcr introduction to pcr technology

DNA

DNA

TaqDNA

dNTP

Mg2+


Pcr introduction to pcr technology

MJ Research, Inc.

PTC-100

PTC-150

PTC-200

PTC-220

PTC-225


Pcr introduction to pcr technology

ABS, Inc.

PE-2400

PE-2700

PE-9600

PE-9700


Pcr introduction to pcr technology

PCR

1

2.

3.

4.


Pcr introduction to pcr technology

1

2

3

94

72

55

22

1

2

3

4

5

min

PCR

1~3

25~30

DNA

2

DNA

100

DNA

DNA

DNA


Pcr introduction to pcr technology


Pcr introduction to pcr technology

PCRPCRPCR

PCR (Real-time PCR)

PCRPCRPCR


Pcr introduction to pcr technology

PCR

1. TaqMan

2. LightCycler

3.


Pcr introduction to pcr technology

TaqMan


Pcr introduction to pcr technology

LightCycler


Pcr introduction to pcr technology

P

C

R


Pcr introduction to pcr technology


Pcr introduction to pcr technology


Pcr introduction to pcr technology


Pcr introduction to pcr technology

HBV EDTA200ul

TB

HPV

CT


Pcr introduction to pcr technology


Pcr introduction to pcr technology


Pcr introduction to pcr technology

250C4DEPCRNAase


Pcr introduction to pcr technology

  • DNA

  • RNARNARNA1:45mol/L GITC()RNA


Pcr introduction to pcr technology

  • DNAEDTA

  • RNA GITC

  • RNA


Pcr introduction to pcr technology

  • /-70

  • DNA10mmol/L Tris

  • 1 mmol/L EDTA(pH 7.5-8.0)4

  • RNA-80

  • -20

  • GITCRNA7


Pcr introduction to pcr technology


Pcr introduction to pcr technology


Pcr introduction to pcr technology

  • 70%


Pcr introduction to pcr technology


Pcr introduction to pcr technology


Pcr introduction to pcr technology

(HBV)

  • 1.0 103copies/ml HBV DNA 4.2 109copies/ml

  • 2. HBVDNA> 4.2 109copies/ml> 4.2 109copies/ml

  • < 1.0 103copies/ml< 1.0 copies/ml

  • 6.666E+066.666 106 copies/ml


Pcr introduction to pcr technology

PCR

1.

2. mRNA

3. SNP


Pcr introduction to pcr technology

HBV DNA


Pcr introduction to pcr technology


Pcr introduction to pcr technology

HBV DNA


Pcr introduction to pcr technology

  • HBV


Pcr introduction to pcr technology


Pcr introduction to pcr technology


Pcr introduction to pcr technology

YMDD

YMDD:

YIDD

YVDD


Pcr introduction to pcr technology


Pcr introduction to pcr technology

DNA

PCR

PCR

PCR


Pcr introduction to pcr technology

DNA

1:

2:


Pcr introduction to pcr technology

/

DNA/RNA

PCR/RT-PCR


Pcr introduction to pcr technology

DNAPCR

Biotin

Primer up

Primer down

Biotin

PCR


Pcr introduction to pcr technology


Pcr introduction to pcr technology

Human Papillomaviruses, HPV

DNA

  • DNA8Kb

  • 5055nm


Pcr introduction to pcr technology

    • 1004030

    • HPV

    • 1HPV 61142

    • 2HPV 16 18313335394551525356585966 68


Pcr introduction to pcr technology

HPV

  • Regoni Stern

  • 1977LavertyHuman papillomavirusHPV

  • 89.Keerti ShahHPV

  • 1995IARCHPV


Pcr introduction to pcr technology

HPV

HPV

  • HPV95%

  • HPV1.55

  • CIN3HPV99.7


Pcr introduction to pcr technology

HPV

  • HPV E6p53

  • HPV E7RBRB E2F

  • HPV16-E5CDKp27


Pcr introduction to pcr technology

HPV

58

HPV HPV

HPV


Pcr introduction to pcr technology

  • HPVHPV

  • PCR18

    5HPV


Pcr introduction to pcr technology

HPV3335

HPV1643


Pcr introduction to pcr technology

HPV

HPV18

Primer up

HPV16

HPV58

Primer down

HPV1643


Pcr introduction to pcr technology

HPV


Pcr introduction to pcr technology

HPV


Pcr introduction to pcr technology

505%80%13.1528.8%

7010.28/10903.25/10


Pcr introduction to pcr technology

4580

354.8200034.1


Pcr introduction to pcr technology


Pcr introduction to pcr technology

100DNA9035301137HPV 1HPV 611424344 2 HPV 16 18 31 3335 3945 51 52 5356 5859 66687383MM43HPV45HPV3959HPV5258

HPV1651HPV185639


Pcr introduction to pcr technology

HPV


Pcr introduction to pcr technology

HPV33

Other

HPV31

HPV45

HPV16

HPV18

HPV

  • HPV611

  • HPV161831334558

HPV


Pcr introduction to pcr technology

HPV

1.1153119

/ 100

226

2. HPV


Pcr introduction to pcr technology

2001TBSThe Bethesda System)

ASCUSAtypical Squamous Cells)

ASC-US:

ASC-H

L-SILHPVCIN I

H-SILCIN IICIN III

SCC

AGC Atypical Glandular Cells)

AISAdenocarcinoma In Situ)

CS

CINCervical Intraepithelial Neoplasm)

CIN I

CIN II

CINIII


Pcr introduction to pcr technology

80

8

/HPV

<30

HPV

HPV

CIN I

415

>30

CIN II/III

6~24

510

HPV

HPV


Pcr introduction to pcr technology

CINCC

CIN


Pcr introduction to pcr technology

HPV5-10

2123HPV5-870103HPV3

25-3065


Pcr introduction to pcr technology

13


Pcr introduction to pcr technology

  • 50-70

  • LCT TCT

    1HPV

  • 2HPV

  • HPV DNAPCR PCR

    (98%)


Pcr introduction to pcr technology

CCRF

pap smear+HPV

TCT+HPV


Pcr introduction to pcr technology

  • HPV


Pcr introduction to pcr technology

HPV35

HPV

HPV


Pcr introduction to pcr technology

CIN 909510

45

8

46HPV


Pcr introduction to pcr technology

21

30

1

HIV


Pcr introduction to pcr technology

3

24


Pcr introduction to pcr technology


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