Maternofetal transmission of human parvovirus b19 genotype 3 in ghana west africa
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Maternofetal transmission of human parvovirus B19 genotype 3 in Ghana, West Africa. D Candotti, K Danso, A Parsyan, A Dompreh, J-P Allain. National Blood Service, England Div. Transfusion Medicine, University of Cambridge, UK

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Maternofetal transmission of human parvovirus B19 genotype 3 in Ghana, West Africa

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Maternofetal transmission of human parvovirus b19 genotype 3 in ghana west africa

Maternofetal transmission of human parvovirus B19 genotype 3 in Ghana, West Africa

D Candotti, K Danso, A Parsyan, A Dompreh, J-P Allain

National Blood Service, England

Div. Transfusion Medicine, University of Cambridge, UK

Dept. Obstetrics & Gynaecology and Dept. Microbiology, Komfo Anokye Teaching Hospital, Kumasi, Ghana


B19 infection in ghana

B19 infection in Ghana

• 82% seroprevalence in adults

• B19 DNA prevalence:

- 1.3% in blood donors

- 11.5% in children (average age: 23 months)

• 0.9% persistent infections:

- IgG only

- low viral load ( 102- 105 IU/ml)

• Endemic region for B19 genotype 3


Phylogenetic tree of full length human parvovirus b19 genomes

Phylogenetic tree of full length human parvovirus B19 genomes

Genotype 2

100

Genotype 1

100

100

3A

100

V9

100

D91.1

Genotype 3

3B

0.005 substitutions/site


Study design

Study design

Pregnant women plasmas

(n=885)

Anti-VP2 IgG

B19 DNA screening

Multiplex QPCR assay

Single QPCR & nested PCR confirmation

Cord blood plasmas

Newborn whole blood

Sequencing

Genotyping

Specific QPCR & nested PCR


Serological and viral markers in pregnant women

Serological and viral markers in pregnant women

Anti-VP2 IgG reactive

144/177

(81%)

Confirmed DNA pos.

16/885

(1.8%)

DNA load (IU/ml)

Median

Range

1.3E+03

46 - 3.6E+06

IgM reactive

IgG reactive

3/16

16/16


Maternofetal transmission

Viral load

(IU/ml)

IgM

titer

IgG

titer

Viral load

(IU/ml)

IgM

titer

Maternofetal transmission

Sample

ID

Type

Mother

Cord blood

Newborn

Viral load

(IU/ml)

1964

2005

1655

1956

1774

1941

2246

2029

2106

1818

2196

1447

2409

2242

2143

1577

3

3

3

3

3

3

3

1

3

3

3

3

3

3

3

3

1.1E+04

1.2E+06

3.6E+06

5.9E+03

4.2E+03

2.1E+03

1.5E+03

1.3E+03

1.2E+03

1.1E+03

6.5E+02

5.6E+02

3.3E+02

5.8E+01

4.6E+01

4.6E+01

1:1000

1:1000

1:100

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

1:1000

1:1000

1:100000

1:10000

1:1000

1:10000

1:100

1:1000

1:1000

1:100

1:1000

1:1000

1:1000

1:1000

1:100

1:100

1.1E+03

2.1E+06

0

0

0

0

0

0

0

0

0

0

0

0

0

0

<1:10

1:1000

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

<1:10

1.7E+03

-

0

-

-

-

-

-

-

-

0

-

0

-

-

-


Conclusions

Conclusions

• B19 genotype 3 is dominant in Ghana and is characterised by high genetic variability

• 2/3 cases of primary maternal B19 infection resulted in vertical transmission

• No evidence that maternal persistent B19 is vertically infectious


Acknowledgements

Acknowledgements

Pr J.-P. Allain

Dr A. Parsyan

Div. of Transfusion Medicine

University of Cambridge

Cambridge, UK

Dr K. Danso

Dept. of Obstetrics & Gynaecology

Komfo Anokye Teaching Hospital

Kumasi, Ghana

Mr A. Dompreh

Serology laboratory

Dept. of Microbiology

Komfo Anokye Teaching Hospital

Kumasi, Ghana

Mr C Horn

Plasmacute

Norway

National Blood Service

England


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