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LUSH PHEROMONE

LUSH PHEROMONE. Manhasset Science Research Jillian Wong. NEED. http://www.cdc.gov/Features/dsMalariaSurveillance/dsMalariaSurveillance_450px.gif. http://www.cdc.gov/Features/dsMalariaSurveillance/. Knowledge Base. 5R,6S)-6-Acetoxy-5-hexadecanolide.

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LUSH PHEROMONE

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  1. LUSH PHEROMONE Manhasset Science Research Jillian Wong

  2. NEED http://www.cdc.gov/Features/dsMalariaSurveillance/dsMalariaSurveillance_450px.gif http://www.cdc.gov/Features/dsMalariaSurveillance/

  3. Knowledge Base • 5R,6S)-6-Acetoxy-5-hexadecanolide http://www.pherobase.com/database/compound/compounds-detail-5R6S-6-acetoxy-5-hexadecanolide.php

  4. Knowledge Base The Lush protein is needed in the detection, discrimination, and perception of volatile odors Figure 2: X-ray Crystal Structure of LUSH-cVA complex. The VA is completely enveloped within the protein. http://www.uccc.info/for-healthcare-professional/cancer-center/cores/structural-biology/structural-biology.aspx

  5. Knowledge Base http://www.nsls.bnl.gov/newsroom/science/2004/images/01-Jones-figure1.jpg Fig. 1. a Surface representation of key residues in the LUSH alcohol-binding pocket. The pocket is lined with hydrophobic residues with the exception of Thr57 and Ser52, which form concerted hydrogen bonds with the alcohol. Ethanol is shown in a stick representation. The hydrogen bonds between the alcohol and the hydroxyl groups of Thr57 and Ser52 are shown as yellow dotted lines.

  6. Knowledge Base http://vosshall.rockefeller.edu/reprints/VosshallStensmyrNeuron05.pdf

  7. Literature Review • Pingxi Xu- A Drosophila OBP Required for Pheromone Signaling

  8. Literature Review • Dean P. Smith- A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate-Induced Responses in Drosophila

  9. METHODOLOGY Drosophila melanogaster Mutant Drosophila Regular Drosophila Group 4 N=20 Group 3 N=20 Group 2 N=20 Group 1 N=20 Group 5 N=20 Group 6 N=20 Group 7 N=20 Group 8 N=20 Placed in oviposition trap 1 µl of Acetoxy-5-hexadecanolide inserted into the food 1.5 µl of Acetoxy-5-hexadecanolide inserted into the food 2 µl of Acetoxy-5-hexadecanolide inserted into the food 2.5 µl of Acetoxy-5-hexadecanolide inserted into the food Data will be analyzed using SPSS and Scheffe-post-hoc test.

  10. Do-ability • Drosophila are readily available, easy to keep alive • Does not require extremely advanced special equipment

  11. Bibliography • Department of Pharmacology and Center for Basic Neuroscience, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9111, USA • Ha, Tal Soo, and Dean P. Smith. "A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate Induced Responces in Drosophila." The Journal Of Neuroscience (2006): 8727-733. • Jin, Xin, Tal Soo Ha, and Dean P. Smith. "PNAS." SNMP is a signaling component required for Pheromone Sensitivity in Drosophila 31st ser. 105 (Ausgust 5, 2008): 10996-1001. • "Pheromone." McGraw-Hill Encyclopedia of Science & Technology. 8th ed. 13 vols. Mechanicsburg: McGraw-Hill, 1997. 397-98. • Smith, Dean P. "Odor and Pheromone detection in Drosophila melanogaster." Pflugers Arch-Eur J Physiol (2007): 749-58. • University Of Texas Southwestern Medical Center At Dallas. "Researchers Find How Protein Allows Insects To Detect And Respond To Pheromones." ScienceDaily 27 January 2005. 4 May 2009 <http://www.sciencedaily.com­ /releases/2005/01/050124005139.htm>. • UT Southwestern Medical Center. "Unique Pheromone Detection System Uncovered." ScienceDaily 27 June 2008. 29 April 2009 <http://www.sciencedaily.com­ /releases/2008/06/080626125825.htm>. • Xu P-X, Atkinson R, Jones DNM, Smith DP (2005) Drosophila OBP LUSH is required for activity of pheromone sensitive neurons. Neuron 45:193-200

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