بسم الله الرحمن الرحيم
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بسم الله الرحمن الرحيم. GENUS: CORYNEBACTERIUM Prof. Khalifa Sifaw Ghenghesh. Diptheroides = Coryneforms Gram +ve, Nonmotile Rods. Arranged in V forms or Palisades Catalase +ve. 1. Corynebacterium diphtheriae. Diphtheria. Pleomorphic Bacilli Cells Contain Metachromatic Granules.

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بسم الله الرحمن الرحيم

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بسم الله الرحمن الرحيم

GENUS: CORYNEBACTERIUM

Prof. Khalifa Sifaw Ghenghesh


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  • Diptheroides = Coryneforms

  • Gram +ve,

  • Nonmotile Rods.

  • Arranged in V forms or Palisades

  • Catalase +ve


1 corynebacterium diphtheriae

1. Corynebacterium diphtheriae

  • Diphtheria.

  • Pleomorphic Bacilli

  • Cells Contain Metachromatic Granules.

Morphology


Corynebacterium diphtheriae club shaped gram positive rods

Corynebacterium diphtheriaeClub-shaped, Gram-positive rods


Cultural characteristics

Cultural Characteristics

  • i. Loeffler's Serum Slope.

  • ii. Tellurite BA.

    - Temp. 37oC,

    - Aerobic and Facultative AnO2.


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Corynebacterium diphtheriae forms black colonies on tellurit agar (left), on blood agar colonies appear white (right)


Pathogenicity

PATHOGENICITY

  • Diphtheria is a Toxaemia.

    • Exotoxin: M. wt 62000

    • 2 Fragaments >> A & B

    • Inhibits Protein Synthesis.

    • Has Special Affinity for Certain Tissues "Mycocardium, Adrenals, Nerve Endings".

  • Toxin >>Toxoid.

  • Site of Infection >>Faucial.

    • Toxin >>Pseudomemebrane.


Laboratory diagnosis

LABORATORY DIAGNOSIS

  • Important>>Specific Treatment Should Be Started Immediately on Suspicion of Diphtheria.

  • Specimen:Two Swabs from the Lesion.

  • Culture:

    i. Loeffler's Serum Slope >> Albert Stain.

    ii. Tellurite BA.

    iii. BA>> Streptococcal or Staphylococcal Pharyngitis.


Pathogenicity tests

PATHOGENICITY TESTS

  • In-vivo Tests:

    i. Subcutaneous Test:

    ii. Intracutaneuos Test:

  • In-vitro Test:

    • Elek Test ( Gel Precipitaion Test).

  • Tissue Culture:


Treatment

TREATMENT

  • i. Antitoxin:> Moderate Cases:

    > Severe Cases:

  • ii. Antibiotic:

  • i. Active Immunization >> PDT.

  • ii. Passive Immunization:

  • iii. Combined:

PREVENTION


Schick test

SCHICK TEST

  • > Injection of 0.2 ml of Diphtheria Toxin Intradermally.

  • > Positive Reaction >> Erythema + Swelling


Schick test1

SCHICK TEST

______________________________________________________________________

ResultTest ArmControl ArmInterpretation Diphthri (Toxin) Inactivated Toxin Immunization

36h 120h 36h 120h

______________________________________________________________________

Negative-------- Immune, Not

Not Hypersensitive Required

----------------------------------------------------------------------------------------

Positive++---- Not Immune, Not Required

Hypersensitive

---------------------------------------------------------------------------------------------------------------------

Negative+--+-- Immune Not

Hypersensitive Required

---------------------------------------------------------------------------------------------------------------------

Positive&+++-- Not ImmuneContra-

PseudoHypersensitive indicated

______________________________________________________________________


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بسم الله الرحمن الرحيم

Listeria monocytogenes


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  • Listerosis.

  • G+ve Coccobacilli >in Short Chains.

  • Motile at 25oC But Not at 37oC.

  • Aerobic to Microaerophilic.

  • On BA>Narrow Zone of b-Haemolysis.

  • Listerolysin O >


Disease

DISEASE

  • Incidence and Transmission >

  • i. In Adults:

  • ii. Neonatal Infections:

    a. Early Type:

    b. Late Type:


Laboratory diagnosis1

LABORATORY DIAGNOSIS

  • i. Specimen:Depend on Syndrome: Blood, CSF, Genital Tract Secretions.

  • ii. Media:Selective Media.

  • Penicillin, Ampicillin.

TREATMENT


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