Expression of microRNAs-192 and -215 in human gastric cell lines
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Expression of microRNAs-192 and -215 in human gastric cell lines. Supplementary Figure 1. Cell cycle assay. A. B. NCI-N87. HFE145. NSC-mim. NSC-inh. G1: 56.63±0.86% S: 20.07±2.39% G2/M: 23.13±2.51%. G1: 62.67±1.07% S: 17.37±1.42% G2/M: 20.17±0.42%. 192-mim. 192-inh.

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Supplementary figure 1

Expression of microRNAs-192 and -215 in human gastric cell lines

Supplementary Figure 1


Supplementary figure 1

Cell cycle assay

A

B

NCI-N87

HFE145

NSC-mim

NSC-inh

G1: 56.63±0.86%

S: 20.07±2.39%

G2/M: 23.13±2.51%

G1: 62.67±1.07%

S: 17.37±1.42%

G2/M: 20.17±0.42%

192-mim

192-inh

G1: 55.87±0.85%

S: 19.77±2.46%

G2/M: 23.77±1.69%

G1: 64.43±0.42%

S: 16.27±1.10%

G2/M: 18.93±1.75%

Cell count

215-mim

215-inh

G1: 55.47±0.84%

S: 20.03±2.31%

G2/M: 23.87±1.54%

G1: 65.00±0.36%

S: 16.20±1.39%

G2/M: 18.40±1.39%

DNA content

Supplementary Figure 2


Supplementary figure 1

Propidium Iodide

Annexin-V-FITC

Apoptosis assay

A

B

HFE145

NCI-N87

NSC-mim

NSC-inh

2.73±0.23%

2.47±0.23%

192-mim

192-inh

2.70±0.26%

3.50±0.36%

215-mim

215-inh

2.57±0.21%

2.73±0.29%

Supplementary Figure 3


Supplementary figure 1

Correlation between ALCAM mRNA levels and microRNA-192 and -215 expression in human gastric tissues

r=-0.0621, p=0.65

r=-0.2043, p=0.13

Supplementary Figure 4


Supplementary figure 1

A

B

C

ALCAM expression by immunohistochemistry in human gastric tissue

Supplementary Figure 5


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