Loading in 5 sec....

DNA PACKING: Characterizing Intermolecular Contacts of DNA Bryson W. Finklea St. John's CollegePowerPoint Presentation

DNA PACKING: Characterizing Intermolecular Contacts of DNA Bryson W. Finklea St. John's College

- 67 Views
- Uploaded on

Download Presentation
## PowerPoint Slideshow about ' DNA PACKING: Characterizing Intermolecular Contacts of DNA Bryson W. Finklea St. John's College' - fitzgerald-trevino

**An Image/Link below is provided (as is) to download presentation**

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.

- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript

Characterizing

Intermolecular Contacts

of DNA

Bryson W. Finklea

St. John's College

DIMACS REU

- Background
- Symmetry
- My Project

- Background
- Symmetry
- My Project

Different representations of the same DNA

(18 base pairs color-coded according to base identity)

(http://siggy.chem.ucla.edu/~tim/chemistry/DNA.jpg)

In nature each human cell has

3 billion DNA base pairs

(about 2 meters long)

(Human Genome Project Information of the DOE)

In nature each human cell has

3 billion DNA base pairs

(about 2 meters long)

Cube built from DNA

in nanotechnology lab

(Human Genome Project Information of the DOE) (Dr. Nadrian Seeman, Department of Chemistry,

New York University)

Molecular Crystals

(often microscopic)*

(www.scripps.edu/newsandviews/e_20010129/chang-2.html)

*These are similar examples from proteins instead of DNA.

Molecular Crystals

(often microscopic)*

DNA X-Ray Diffraction Pattern*

(www.scripps.edu/newsandviews/e_20010129/chang-2.html)

(http://userpage.chemie.fu-berlin.de/~psf/ifv_psfx.htm)

*These are similar examples from proteins instead of DNA.

- Background
- Symmetry
- My Project

- Crystal –
- a solid with regularly repeating arrangement of atomsUnit Cell –
- the basic unit of symmetry
- an arrangement of atoms
- that repeats in every direction

(Unknown)

wallpaper pattern

To show symmetry:

- pick a point
- find all equivalent points

wallpaper pattern

To show symmetry:

- pick a point
- find all equivalent points
- the points form a 2D lattice

wallpaper pattern

- Connecting 4 lattice points to form a parallelogram gives a possible unit cell
- Unit cell – the basic unit that repeats in every direction
- Different unit cells can be chosen
- But some unit cells are preferable for higher symmetry

- Symmetry is defined bysymmetry elements
- Four possible symmetry elements in 2D:
- Rotation points (by 60°, 90°, 120°, or 180°)
- Reflection axes
- Glide reflection axes (reflection and translation)
- Inversion points
- (Translation)
- Symmetry operations –the actual changes carried out
- in relation to a symmetry element

wallpaper pattern

Reflection Axis

Glide Reflection Axis

90° Rotation Point

180° Rotation Point

Symmetry elements of this wallpaper group

(http://www.clarku.edu/~djoyce/wallpaper/)

wallpaper pattern

- Unit cell

Reflection Axis

Glide Reflection Axis

90° Rotation Point

180° Rotation Point

Symmetry elements of this wallpaper group

wallpaper pattern

- Unit cell*

- Asymmetric Unit –the simplest unit on which the symmetry operations can act to produce the entire symmetrical structure*

Reflection Axis

Glide Reflection Axis

90° Rotation Point

180° Rotation Point

Symmetry elements of this wallpaper group

* Although the spirit of what I show is correct, it appears from the following website that my choice of conventional unit cell and choice of asymmetric unit may be unconventional or even wrong. See the last example in the n=4 section of the following website: http://jwilson.coe.uga.edu/EMT668/EMAT6680.F99/McCallum/WALLPA~1/SEVENT~1.HTM

- Crystal –
- a solid with regularly repeating arrangement of atomsUnit Cell –
- the basic unit of symmetry
- an arrangement of atoms
- that repeats in every direction
- (Different colors are different copies of the same asymmetric unit)

- Six symmetry elementsin 3D:
- Rotation axes (by 60°, 90°, 120°, or 180°)
- Reflection planes
- Glide reflection planes (reflection and translation)
- Inversion points
- (Translation)
- Screw Axes (translation and rotation)
- Rotary inversion axes (rotation and inversion)
- Sets of symmetry operations form algebraic groups called
- space groups.
- 230 space groups

Asymmetric unit Unit cell 27 adjacent unit cells

- Background
- Symmetry
- My Project

Characterizing Intermolecular Contacts of DNA

Data from Nucleic Acid Database (NDB):

- orthogonal coordinates of atoms in an asymmetric unit
- equivalent positions in equation form (info from symmetry elements)
- unit cell dimensions and angles
To revise a computer program to:

- reconstruct coordinates of the atoms in a unit cell
- …then in a 3x3x3 block of unit cells
- make measurements of interesting properties of contacts between
molecules of DNA (Examples: distances, angles between axes,…)

Asymmetric unit Unit cell 27 adjacent unit cells

- Details on computer program structure
and images created using its output

- Specification of important DNA molecular contacts
and report of findings

- Perhaps more details on mathematics of space groups, including notation used

DNA for the layman:

Understanding DNA, Calladine and Drew, 3rd edition.

Symmetry in crystals, including space group theory:

Crystal Structure Analysis for Chemist and Biologists, Glusker, et al, Ch. 1, 2, and 4.

X-Ray Analysis and the Structure of Organic Molecules,

Dunitz, Ch. 2.

Molecular structure databases (on web):

Nucleic Acid Database (NDB), Protein Data Bank (PDB),

Cambridge Structural Database (CSB)

DIMACS REU

NSF Support

Advisor:

Wilma Olson, Department of Chemistry,

Rutgers University

Additional Advisors:

A.R. Srinivasan, Department of Chemistry

Rutgers University

Andrew Colasanti, Department of Molecular Biology

Rutgers University

(background: http://www.karolinskaeducation.ki.se/services/courses/selection_courses_se.html)

Download Presentation

Connecting to Server..