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Fluid phases

Fluid phases. Area A, hydrophobic thickness 2D C , and bending modulus K C for fully hydrated fluid (L a ) lipid bilayers (5/2011). Lipid T ( o C) D HH (Å) A (Å 2 ) 2D C (Å) K C /kT

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Fluid phases

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  1. Fluid phases Area A, hydrophobic thickness 2DC, and bending modulus KC for fully hydratedfluid (La) lipid bilayers (5/2011) Lipid T (oC)DHH (Å) A (Å2) 2DC(Å) KC/kT DPPC 50 38.0d 62.9(±1.3)a,64.0b,64.3c,63.1d* 29.2a,28.5b,27.9c,28.4d* 15.0**-e DHPC 48 38.2e 65.1e 27.6e 9.4e DLPE 35 35.6b 51.2b 25.8b - DMPC 30 35.3g 59.7f,60.6g 26.2f,25.4g 15.8**-g DLPC 30 30.8g63.2g20.9g 13.2g DOPC 30 36.7d 72.2h,72.5b,72.1i,72.4j,k,67.4d*27.2h,27.1b, 27.2i,26.8j,k,28.8d*18.2** to be rescaled(15) 37.6k 69.1k 27.7k 20.3k T trends correct(45) 36.1k 75.5k 26.2k 17.2k DOPS 30 38.4l65.3l30.2l - EggPC 30 35.4f69.4f,b 27.2f,27.1b - POPC 30 37.0j68.3(±1.5)j27.1j 20.3j SOPC 30 39.1m 67.0(±0.9)m 29.2(±0.4)m 21.5m diC22:1PC 30 44.4j 69.3j 34.4j 30.4j 18:0-22:5PC 24 37.9n68.7n30.5n 25.6** 18:0-22:6PC 24 37.9n68.2n30.5n 18.9** DiphytanoylPC 30 36.4r 80.5(±1.5)r 27.2r 12.4r Typical uncertainties 0.1 0.1 0.5-1.0 0.3-0.5 0.5-1.0 The most robust thickness from X-ray is the head-head thickness DHH. Numbers for 2DC and A are being critically reviewed and updated. In particular, it is now clear from ref d thatfor DOPC thesmallest value of A and the largest value of 2DC are the ones in agreement with neutron scattering; other temperatures should be rescaled. Similar reanalysis for DPPC did not make such a large difference, but other lipids have not yet been similarly reanalyzed. The product of A and 2DC is more robust than either alone. aBiophys.J. 70:1419(1996); bBiochim.Biophys.Acta: Reviews on Biomembranes 1469:159(2000); cBiophys.J.:Biophys.Lett 90:L83(2006); dBiophys.J. 95:2356(2008); eChem.Phys.Lipids 160:33(2009); fChem.Phys.Lipids 95:83(1998); gBiophys.J. 88:2626(2005); hBiophys.J. 75:917(1998); iPhys.Rev.E 69:040901(2004); jJ.Membr.Biol. 208:193(2005); kBiophys.J. 94:117(2008); lBiophys.J. 86:1574(2004); mBiochim.Biophys.Acta 1178:1120(2008); nJ.Am.Chem.Soc. 125:6409(2003); oBiophys.J. 83:3324(2002); pBiophys.J. 64:1097(1993); qBiophys.J. 71:885(1996); rChem.Phys.Lipids 163:630(2009).*Neutron data; **Upon reanalysis(2009) See next page for older gel phase results

  2. Gel phases Fully hydrated gel phase lipid bilayer areas and hydrophobic thicknesses (9/2010) Lipid T(oC) A(Å2) 2DC (Å) DMPC 10 47.2o 30.3(±0.2)o DiC16PC,18,20,22,24 20 47.5p,q 34.4b,37.1q,40.7q,44.0q,48.0q DMPS 20 40.8l 36.0l DLPE 20 41.0b30.0b DHPC-Interdig. 20 77.2e 20.3e DHPC-gel 20 46.9e 34.6e aBiophys.J. 70:1419(1996); bBiochim.Biophys.Acta: Reviews on Biomembranes 1469:159(2000); cBiophys.J.:Biophys.Lett 90:L83(2006); dBiophys.J. 95:2356(2008); eChem.Phys.Lipids 160:33(2009); fChem.Phys.Lipids 95:83(1998); gBiophys.J. 88:2626(2005); hBiophys.J. 75:917(1998); iPhys.Rev.E 69:040901(2004); jJ.Membr.Biol. 208:193(2005); kBiophys.J. 94:117(2008); lBiophys.J. 86:1574(2004); mBiochim.Biophys.Acta 1178:1120(2008); nJ.Am.Chem.Soc. 125:6409(2003); oBiophys.J. 83:3324(2002); pBiophys.J. 64:1097(1993); qBiophys.J. 71:885(1996); rChem.Phys.Lipids 163:630(2009).

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