1 / 12

Published: 02 September 2005 Journal of Carcinogenesis 2005, 4 :15 doi:10.1186/1477-3163-4-15

Apoptosis induced by the Tibetan herbal remedy PADMA 28 in the T cell-derived lymphocytic leukaemia cell line CEM-C7H2 Marcel Jenny1, Wolfgang Schwaiger1, David Bernhard2, Oliver A Wrulich1, Daria Cosaceanu3, Dietmar Fuchs4 and Florian Ueberall*1. Published: 02 September 2005

findlay-gavin
Download Presentation

Published: 02 September 2005 Journal of Carcinogenesis 2005, 4 :15 doi:10.1186/1477-3163-4-15

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Apoptosis induced by the Tibetan herbal remedy PADMA 28 in theT cell-derived lymphocytic leukaemia cell line CEM-C7H2Marcel Jenny1, Wolfgang Schwaiger1, David Bernhard2, Oliver A Wrulich1,Daria Cosaceanu3, Dietmar Fuchs4 and Florian Ueberall*1 Published: 02 September 2005 Journal of Carcinogenesis 2005, 4:15 doi:10.1186/1477-3163-4-15 Received: 12 August 2004 Accepted: 02 September 2005 This article is available from: http://www.carcinogenesis.com/content/4/1/15 c 2005 Jenny et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 顏文笙

  2. 摘要 PADMA 28 (P28)為一種西藏的草藥，原用在治療 -動脈硬化(atherosclerosis) -慢性肝炎(chronic hepatitis) -呼吸道感染(infection of the respiratory) 具有抗氧化的特性。本篇文章使用-血癌細胞 T cell-derived lymphocytic leukaemia cell line CEM-C7H2 來看P28所造成的效果 P28主要在細胞造成的現象有 1-抑制細胞增生 2-造成poly (ADP-ribose) polymerase ---PARP fragmentation 3-apoptosis 4-P28干擾Bcl-2的功能

  3. Composition of PADMA 28

  4. Stock solution Of PADMA 28 *EtOH 70% *MeOH *DMSO (dimethylsulfoxide) *PBS (phosphate-buffered saline)

  5. ＊現象＊ 抑制細胞增生

  6. Apoptosis

  7. ＊現象＊PARP的fragmentation PARP為一種保護DNA的蛋白，保護DNA不被Lysis

  8. Caspase是引發apoptosis的蛋白，當它是全整的時候是失活的。但是一但被切開就活化，會一步步活化下去。Caspase是引發apoptosis的蛋白，當它是全整的時候是失活的。但是一但被切開就活化，會一步步活化下去。

  9. ＊現象＊ P28干擾Bcl-2的功能

  10. 判定P28在Bcl-2的上or下游作調控

  11. Discussion *作者提出假設，是否P28活化了caspas。進而導致PARP cleavage *Bcl-2有部份的表現是由INF-gama控制，作者推測P28是在上游作調控。 *PADMA 28 抑制 cell 的增生 *Apoptosis 的情形要看cell culture的情形 *化療的功效來自於，化合的概念來治療cancer。 　這和中藥上，複方上的觀念很類似，而且可以降低化療所造成的毒性傷害 *中草藥在萃取和分餾的過程中，會破壞成份 *無明確的結果在人體上的一般細胞 *中草藥並非都是有效且專一的，也無法在血液中固定梯度，只能控制成份在合理的範圍內

More Related