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实验十三 血乳酸的测定与应用 PowerPoint PPT Presentation


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实验十三 血乳酸的测定与应用. 1 实验目的. ⑴ 通过实验使学生掌握体内血乳酸的测定方法及该指标在运动实践中的应用和意义,为体育教学和运动训练提供指导。 ⑵进一步巩固离心机、可调微量移液器、振荡器等仪器的基本操作。掌握实验对象取材、标本处理的方法与半自动生化分析仪的操作方法。 ⑶通过综合设计型实验的实验设计与研究过程,提高学生的学习兴趣,培养学生查阅文献资料、独立思考、创新思维、动手操作、分析问题和解决问题的能力,使学生基本掌握从事一般科学研究的方法。. 2 实验原理. 2 实验原理.

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实验十三 血乳酸的测定与应用

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7071583


7071583

1


7071583

2

2

  • 0.45-1.3mmol/L


7071583

  • 400m10 mmol/L14-15 mmol/L


7071583

3

3


7071583

IgG

0.05 mol/LpH9.63Na2CO30.15gNaHCO20.293g100ml

PBS-TweenNaCl8gKCl0.2gKH2PO4 0.2gNa2HPO412H2O 2.9gTween-200.5ml1000ml

0.5%BSAPBS

0.1 mol/L2.1g/100 ml6.1 ml0.2 mol/L Na2HPO412H2O7.163 g/100 ml8 mg30%H2O240 l

2mol/L H2SO4


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4

4

  • 4-5


7071583

enzyme-linked immunosorbent assay, ELISARadioimmuniassay,RLA

NAD+LDHNAD+NADHPMSNBT530nm TP


7071583

3-17


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5

5

10

585-95

1-10 g/200 l37 1 h416-18 h


7071583

200l3

200l371h

200l3712h2 h

IgG200l371h2h

200l5minA190 nm


7071583

0.45-1.3 mmol/L10-15 mmol/LP<0.05

400 m10 mmol/L14-15 mmol/L


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6

6


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