Staining HAEMATOXYLIN & EOSIN. Supervised by : Dr.Sherifa Prepared by: Reem Aldossari Lama Alabdi. H&E staining:
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Supervised by : Dr.Sherifa
Prepared by: Reem Aldossari
H&E staining is the standard staining method used in histology. It gives an overview of the structure of the tissue, enabling differentiation of the structures being examined as normal, inflammed,degenerative and pathological changes .
10-EtOH(ethanol alcohol) 70% (2-3 minutes)
11-EtOH(ethanol alcohol) 80% (2-3 minutes)
13-Eosin Y solution, 2 to 5 minutes.
16- Absolute 100% ethanol, 1-2 minutes.
18-Xylene I, 1-2 minutes.
21-Mounting with DPX