Lab 5

1. Lab 5 ALS & Trigeminal Systems

2. 1 Marginal Zone Substantia gelatinosa 2 Mid-lumbar level of spinal cordCresyl violet stain for cell bodies (Anatomical Orientation)

3. 1 Ventral white comissure Substantia Gelatinosa (very white) 2

4. 1 Ventral white comissure Substantia Gelatinosa (relatively white) 2

5. 3 raphe nuclei 2 ML 5 ML 1 ALS 4 ALS What brainstem level is shown here? The ALS & ML labels represent sensation from which side of the body? Rostral Medulla Left

6. 2 Medial lemniscus 1 ALS 4 Medial lemniscus ALS 3 ALS What brainstem level is shown here? Caudal Pons

7. ALS 1 Medial Lemniscus 2 ALS Medial Lemniscus 5 ALS 4 3 PAG PAG 6 What brainstem level is shown here? Rostral Midbrain

8. 1 Internal medullary lamina + Intralaminar nuclei 3 General location: Internal medullary lamina + intralaminar nuclei Posterior limb, internal capsule 2

9. What is the approximate location of the: Substantia Gelatinosa? What spinal level is shown here? Sacral (again, probably don’t need to know) Marginal Zone? Ventral White Commisure? ALS?

10. What is the approximate location of the: Substantia Gelatinosa? What spinal level is shown here? Lumbar Marginal Zone? Ventral White Commisure? ALS?

11. What is the approximate location of the: Substantia Gelatinosa? What spinal level is shown here? Thoracic Marginal Zone? Ventral White Commisure? ALS?

12. What is the approximate location of the: Substantia Gelatinosa? What spinal level is shown here? Cervical Marginal Zone? Ventral White Commisure? ALS?

13. What is the approximate location of the: ALS? What brainstem level is shown here? Caudal Medulla Reticular Formation? Poorly defined area.

14. What is the approximate location of the: ALS? What brainstem level is shown here? Mid/Rostral Medulla Reticular Formation?

15. What is the approximate location of the: ALS? What brainstem level is shown here? Rostral Medulla Reticular Formation? Raphe Nucleus? Decending Pain Block (Serotonin or NorEp)

16. What is the approximate location of the: ALS? What brainstem level is shown here? Caudal Pons Reticular Formation? Raphe Nucleus?

17. What is the approximate location of the: ALS? What brainstem level is shown here? Rostral Pons Reticular Formation? Raphe Nucleus?

18. What is the approximate location of the: ALS? What brainstem level is shown here? Rostral Pons Reticular Formation? Medial Lemniscus?

19. What is the approximate location of the: ALS? What brainstem level is shown here? Rostral Midbrain Reticular Formation? PAG?

20. Contains fibers from both: ALS and Medial Lemniscus What is the approximate location of the VPL? What part of the brain is shown here? Thalamus

21. What is the approximate location of the ALS?

22. What is the approximate location of the ALS?

23. What is the approximate location of the ALS?

24. What is the approximate location of the ALS?

25. Trigeminal System

26. 0 C.N. V nerve roots, trigeminal nerve Medial lemniscus VTTT 1 2 Motor (medial) & main sensory (lateral) nuclei of V, separated by C.N. V nerve roots (black line) 3 What level of the brainstem is shown here? Rostral Pons

27. Spinal trigeminal tract 2 Spinal trigeminal nucleus General position of spinal trigeminal tract (lateral) and spinal trigeminal nucleus (medial) – in lateral tegmentum 1 What level of the brainstem is shown here? Caudal Medulla

28. 1 POSITION OF MEDIAL LEMNISCUS (outlined in red) ALS 2 Spinal trigeminal nucleus 3 ALS 5 Spinal trigeminal tract 7 Spinal trigeminal tract 4 Spinal trigeminal nucleus 6 What level of the brainstem is shown here? Rostral Medulla

29. Medial lemniscus 2 1 ALS 6 Medial lemniscus 5 ALS spinal trigeminal nucleus 7 8 spinal trigeminal tract Spinal trigeminal nucleus 3 Spinal trigeminal tract 4 What level of the brainstem is shown here? Caudal Pons

30. ALS Medial Lemniscus 1 ALS 2 Medial Lemniscus 7 5 ALS location of the VTTT 3 4 PAG PAG 8 location of the VTTT 6 What level of the brainstem is shown here? Rostral Midbrain

31. Medial Lemniscus ML ML VTTT VTTT ALS ALS PAG VTTT What is the approximate location of the ML, ALS, & VTTT in the figure on the left? What is the approximate location of the ML, ALS, & VTTT in the figure on the right?

32. Spinaltrigeminal Nucleus – sends axons that cross & accend to VPM Spinaltrigeminal tract – ipsilateral pain and temp from face and teeth What is the approximate location of the: Spinal Trigeminal Tract? What brainstem level is shown here? Caudal Medulla Spinal Trigeminal Nucleus?

33. Axons cross and ascend in to VTTT at the caudal pons and continue to the VPM What is the approximate location of the: Spinal Trigeminal Tract? What brainstem level is shown here? Mid/Rostral Medulla Spinal Trigeminal Nucleus?

34. What is the approximate location of the: Spinal Trigeminal Tract? What brainstem level is shown here? Rostral Medulla Spinal Trigeminal Nucleus?

35. Main Motor Nucleus Supplies muscles of mastication Trigeminal Roots Main Sensory Nucleus Discriminative touch Receives fibes from trigem ganglion Sends axons across midline to VTTT Mixed VTTT Contralateral spinotrgeminal tract (Ipsilateral) What is the approximate location of the: Trigeminal Main Sensory Nucleus? What brainstem level is shown here? Trigeminal Main Motor Nucleus? Rostral Pons VTTT?

36. What is the approximate location of the VTTT? What brainstem level is shown here? Rostral Midbrain

37. VPM = sensory relay nucleus for general face sensation and for taste What is the approximate location of the: VPM? What part of the brain is shown here? Thalamus Posterior Limb of the Internal Capsule?