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Evaluation of Advanced GEM Lines for Multiple Insect Resistance and Fumonisin Concentration. Martin Bohn Crop Sciences University of Illinois. Objectives. Evaluate GEM lines for their resistance against WCR as well as first and second generation ECB.

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slide1

Evaluation of Advanced GEM Lines for Multiple Insect Resistance and Fumonisin Concentration

Martin Bohn

Crop SciencesUniversity of Illinois

slide2

Objectives

Evaluate GEM lines for their resistance against WCR as well as first and second generation ECB.

Evaluate GEM lines for their resistance against Fusarium species.

Determine the association between insect resistance and fumonisin concentration in GEM germplasm.

slide3

Objectives

Study the genetic basis of insect resistance in maize against both insect species by diallel crosses and testcrosses, and

Initiate a recurrent selection program aimed to develop new maize lines with improved multiple insect resistant (MIR).

slide4

Maize

WCR

ECB

GEM-Pop. (25%)

GEM-Pop. (100%)

Approach

slide5

Materials and Methods

“Population” 15 Entries

“Inbred” 20 Entries

Location: Urbana

Design: α – lattice, 4 replications

Plot size: Population – 4 row plots Inbreds - 2 row plots

Row size: 1.50m × 0.75m 10-12 plants hand planted

WCR eval.: Trap crop

ECB eval.: 1ECB – 180 eggs / plant 2ECB – 180 eggs / plant

slide6

Materials and Methods: WCR

Node-Injury Scale (0.00 – 3.00)

(Oleson and Tollefson, Iowa State Uni.)

1.50

No. of full nodes eaten

% of a node eaten

0.00

3.00

slide7

Materials and Methods: ECB

Leaf Damage Rating

1 = no visible leaf feeding 9 = long lesions on most leaves

Stalk Damage Ratings

1 = no stalk breakage 9 = stalk breakage beneath the ear

Number of Larvae

Source: D. Barry

Source: T. Magg

slide8

Results: Populations

Root Damage Ratings (0.00 – 3.00)

DKXL212:N11a01

UR10001:N1708b

UR10001:N1702

CH05015:N1204

DKB844:S1612

NGSDCRW1(S2)C4-15-2S2(S1)

FS8A(T):N1804

FS8A(S):S0907

CASH:N1410

AR17056:N2025

AR16026:S1719

AR13035:S11b04

AR17056:S1216

UR13085:N0204

AR16026:N1210

0.60

MIN

0.88

1.00

1.40

MEAN

MAX

1.74

1.80

LSD(5%) = 0.99

http://www.ent.iastate.edu/pest/rootworm/nodeinjury/nodeinjury.html

slide9

Results: Inbreds

CUBA117:S1520-153

AR17056:N2025-728

B64

CUBA117:S1520-182

AR17056:N2025-#5

AR17056:N2025-522

CUBA117:S1520-52

CUBA117:S1520-41

CUBA117:S1520-156

AR17056:N2025-546

AR17056:N2025-508

AR17056:N2025-#2

AR17056:N2025-532

B37

Mo17

AR17056:N2025-#4

NGSDCRW1(S2)C4-15

AR17056:N2025-#1

AR17056:N2025-#3

B73

Root Damage Ratings(0.00 – 3.00)

0.50

MIN

0.56

MEAN

1.08

1.50

1.73

MAX

2.00

LSD(5%) = 0.74

http://www.ent.iastate.edu/pest/rootworm/nodeinjury/nodeinjury.html

slide10

Results: ECB

Evaluation of inbreds derived from GEM populations (25% exotic) for various ECB resistance traits.

Mean SE Min Max

LDR 3.97 0.68 3.18 5.25

SDR1 2.34 0.82 1.82 2.96

SDR2 3.96 0.64 3.04 5.44

1ECB 2.51 0.84 1.85 3.52

2ECB 3.46 0.78 2.45 4.54

SE = Standard error

slide11

Results: ECB

Evaluation of GEM populations (25% exotic) for various ECB resistance traits.

Mean SE Min Max

LDR 4.73 0.23 2.73 7.62

SDR1 4.18 0.60 2.38 6.54

SDR2 2.90 0.71 2.14 4.50

1ECB 4.10 0.46 2.79 7.26

2ECB 3.41 0.65 2.33 5.06

SE = Standard error

slide12

Summary

Results confirm: Populations AR17056:N2025 andCUBA117:S1520 are possible sources of WCR resistance.

Four inbreds derived from populations AR17056:N2025 (2) and CUBA117:S1520 (2) showed significantly (P < 0.05) lower root damage ratings than the resistant check.

Significant (P < 0.01) differences between entries were found for leaf damage ratings. Some inbreds and populations showed intermediate levels of resistance against the first generation ECB.

Differences between entries for stalk damage ratings and number of larvae were not significant.

slide13

Activities in 2004

Repeat experiment!

Screen more GEM germplasm!

Conduct diallel study!

Initiate recurrent selection programs!

slide14

Acknowledgements

Mike Gray

Sue Ratcliffe

Kevin Steffey

Brad Engel

Ricardo Fonseca

And to the “rest” of crew:

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