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http://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php. Chapter 10. DNA, RNA, and Protein Synthesis. Section 1 Vocabulary Pretest. 1. Virulent 2. Transformation 3. Bacteriophage. Viruses that infect bacteria Transfer of DNA fragments from cell to cell

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Chapter 10

http://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.phphttp://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

Chapter 10

DNA, RNA, and Protein Synthesis


Section 1 vocabulary pretest
Section 1 Vocabulary Pretesthttp://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

1. Virulent

2. Transformation

3. Bacteriophage

  • Viruses that infect bacteria

  • Transfer of DNA fragments from cell to cell

  • Virus capable of causing disease


Answer key
Answer keyhttp://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

1. VirulentC

2. TransformationB

3. BacteriophageA


Discovery of dna
Discovery of DNAhttp://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

  • Three experiments led to the discovery of DNA as the hereditary factor that Mendel described in his experiments with pea plants.

    • Frederick Griffith’s Experiment (1928)—showed that hereditary material can pass from one bacterial cell to another (transformation)

    • Oswald Avery’s Experiment (1940s)—showed that DNA is the hereditary material that transfers information between bacterial cells.

    • Alfred Hershey and Martha Chase’s Experiment (1952)– confirmed that DNA, and not protein, is the hereditary material in all cells.


Fig. 16-2http://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

Griffith’s Experiment

Mixture of heat-killed S cells and living R cells

EXPERIMENT

Living R cells (control)

Living S cells (control)

Heat-killed S cells (control)

Protective capsule

Griffith concluded that the

living R bacteria had been

transformed into pathogenic

S bacteria by an unknown,

heritable substance from

the dead S cells that allowed

the R cells to make capsules.

RESULTS

Mouse dies

Mouse healthy

Mouse healthy

Mouse dies

Living S cells


Fig. 16-4-1http://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

EXPERIMENT

Radioactive protein

Phage

Bacterial cell

DNA

Batch 1: radioactive sulfur (35S)

Radioactive DNA

Batch 2: radioactive phosphorus (32P)


Fig. 16-4-2http://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

EXPERIMENT

Empty protein shell

Radioactive protein

Phage

Bacterial cell

DNA

Batch 1: radioactive sulfur (35S)

Phage DNA

Radioactive DNA

Batch 2: radioactive phosphorus (32P)


Fig. 16-4-3http://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

Hershey and Chase’s Experiment

See page 307

EXPERIMENT

Empty protein shell

Radioactivity (phage protein) in liquid

Radioactive protein

Phage

Bacterial cell

DNA

Batch 1: radioactive sulfur (35S)

Phage DNA

Centrifuge

Pellet (bacterial cells and contents)

Radioactive DNA

Batch 2: radioactive phosphorus (32P)

Centrifuge

Radioactivity (phage DNA) in pellet

Pellet


Section 2 vocabulary pretest
Section 2 Vocabulary Pretesthttp://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

  • Nucleotide

  • Deoxyribose

  • Nitrogenous base

  • Purine

  • Pyrimidine

  • Base-pairing rules

  • Complementary base pair

  • Base sequence

  • Sugar found in DNA

  • Consists of a sugar, phosphate and nitrogenous base

  • Single ring nitrogenous base pair

  • Double ring nitrogenous base pair

  • Rule stating: A always pairs w/ T and C always pairs w/ G

  • Order of bases on an DNA strand

  • Contains nitrogen and carbon atoms and is found on the rungs of a DNA ladder

  • A and T C and G


Answer key1
Answer Keyhttp://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

  • NucleotideB

  • DeoxyriboseA

  • NitrogenousbaseG

  • PurineD

  • PyrimidineC

  • Base-pairing rulesE

  • Complementary base pair H

  • Base sequence F


Dna structure
DNA Structurehttp://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

  • By 1950, we knew DNA was the hereditary molecule.

  • How did it work? How did it replicate, store and transmit hereditary information and direct cell function?

  • The answer is found in the unique structure of DNA.

http://www.med.unc.edu/pmbb/DNA_Day/mission.html


http://svhs.ucps.k12.nc.us/academics/science.phphttp://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

  • The structure of DNA was discovered in 1953 by James Watson and Francis Crick.


Deoxyribonucleic acid
Deoxyribonucleic Acidhttp://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

  • Described as a double helix (twisted ladder).

  • Formed by two long strands of repeating subunits called nucleotides.

http://www.biologyjunction.com/nucleotide_model_preap.htm

http://www.biojobblog.com/tags/dna/


http://www.biologyjunction.com/nucleotide_model_preap.htmhttp://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

  • Each nucleotide has three parts:

    • Five-carbon sugar called deoxyribose

    • Phosphate group (phosphorous bonded to 4 oxygens)

    • Nitrogenous base (either adenine, thymine, guanine or cytosine)

http://asm.wku.edu/pix/pix.htm


  • The http://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.phpsides of the ladder are formed by covalently bonding the sugar of one nucleotide to the phosphate of another.

Sugar

Phosphate

Covalent bond

http://academic.brooklyn.cuny.edu/biology/bio4fv/page/molecular%20biology/dna-structure.html


  • The nitrogenous bases form the http://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.phprungs of the ladder.

  • There are four types of nitrogen bases:

    • Thymine

    • Cytosine

    • Adenine

    • Guanine

http://student.ccbcmd.edu/~gkaiser/biotutorials/dna/fg4.html


  • Adeninehttp://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php and Guanine have a double ring of carbon and nitrogen atoms and are called purines.

  • Thymine and Cytosine have a single ring of carbon atoms and nitrogen atoms. They are called pyrimidines

http://blog.dearbornschools.org/biologyblog/2010/02/09/february-9-2010/


  • The bases pair together to form the rungs of the DNA ladder.http://www.3dscience.com/3D_Models/Biology/DNA/DNA_with_Phosphate.php

  • Hydrogen bonds hold them together.

  • They always pair according to the following base-pairing rules discovered by Erwin Chargaff in 1949:

    • A – T

    • C – G

    • Note: since this pairing guarantees that a purine always pairs with a pyrimidine, the rungs are always the same length


http://academic.brooklyn.cuny.edu/biology/bio4fv/page/molecular%20biology/dsDNA.jpghttp://academic.brooklyn.cuny.edu/biology/bio4fv/page/molecular%20biology/dsDNA.jpg


  • The base pairs of A/T and C/G are called http://academic.brooklyn.cuny.edu/biology/bio4fv/page/molecular%20biology/dsDNA.jpgcomplementary base pairs.

  • The order of base pairs on a chain of DNA is called its base sequence.

    • Because of its base pairing pattern, one strand of DNA can serve as a template for making a new complementary strand.

    • This is how DNA replicates itself.


  • Ahttp://academic.brooklyn.cuny.edu/biology/bio4fv/page/molecular%20biology/dsDNA.jpg strand of DNA has the following sequence:

    C T G G A C

    What is the sequence of the complementary strand?

    G A C C T G

http://www.fhcrc.org/education/courses/cancer_course/basic/img/dna.gif


Section 3 vocabulary pretest
Section 3 Vocabulary Pretesthttp://academic.brooklyn.cuny.edu/biology/bio4fv/page/molecular%20biology/dsDNA.jpg

  • DNA replication

  • Helicase

  • Replication fork

  • DNA polymerase

  • Semi-conservative replication

  • Mutation

  • A change in a nucleotide sequence of DNA

  • Enzyme that separates two strands of DNA

  • Enzyme that adds nucleotide bases to copying strands of DNA

  • Point where two DNA strands separate

  • Process of copying DNA

  • DNA replication that results in one old and one new strand in each copied molecule


Answer key2
Answer Keyhttp://academic.brooklyn.cuny.edu/biology/bio4fv/page/molecular%20biology/dsDNA.jpg

  • DNA replicationE

  • HelicaseB

  • Replication forkD

  • DNA polymeraseC

  • Semi-conservative replicationF

  • Mutation A


Dna replication
DNA Replicationhttp://academic.brooklyn.cuny.edu/biology/bio4fv/page/molecular%20biology/dsDNA.jpg

  • DNA replication is the process by which DNA is copied in a cell before a cell divides by mitosis, meiosis, or binary fission.

  • Steps:

    • Helicases (enzymes) separate the DNA strands by breaking hydrogen bonds between base pairs. This creates an open area of DNA called a replication fork.

http://www.nvo.com/jin/nss-folder/scrapbookcell/DNA%20Replication.jpg


  • DNA polymerases http://academic.brooklyn.cuny.edu/biology/bio4fv/page/molecular%20biology/dsDNA.jpg(more enzymes) add complementary nucleotides to each of the original sides. Notice that synthesis on each strand moves in opposite directions.



http://jc-biology.blogspot.com/2011/02/replication-of-dna-summary.html


Speed of replication
Speed of Replication completely

  • DNA adds nucleotides at a rate of 50 per second.

  • However, at this rate it would take 53 days to replicate a large human chromosome.

  • Therefore, replication must begin at several, usually thousands, of different points, or origins, at the same time.


Mutations completely

  • Any change in the nucleotide sequence of a DNA molecule is called a mutation.

    • DNA polymerase can check and correct mistakes made during replication.

    • However, mistakes do happen.

    • Mistakes can be spontaneous or caused by environmental factors (radiation, chemicals, etc.)

    • Mutations can be helpful, harmful or harmless.

    • Mistakes made in genes that control cell division can lead to tumors.


Section 4 vocabulary pretest
Section 4 Vocabulary Pretest completely

  • Ribonucleic acid

  • Transcription

  • Translation

  • Protein synthesis

  • Ribose

  • Messenger RNA

  • Transfer RNA

  • Nucleic acid important in protein synthesis

  • Sugar found in RNA

  • RNA that carries instructions from the nucleus to ribosomes

  • Process of making an RNA molecule from a DNA template

  • RNA that assembles an amino acid chain

  • Process of assembling a protein from a coded RNA message

  • DNA RNA protein


Pretest continued completely

  • An organism’s entire gene sequence

  • Sequence of nucleotides at the end of a gene

  • Sequence of nucleotides that start transcription

  • 3-nucleotide sequence on mRNA that encodes an amino acid

  • 3-nucleotide sequence on tRNA that complements a codon

  • Specifies the amino acid sequence of a protein

  • Enzyme that catalyzes the formation of RNA

  • RNA polymerase

  • Promoter

  • Termination signal

  • Genetic code

  • Codon

  • Anticodon

  • Genome


Answer key3
Answer Key completely

  • Ribonucleic acid A

  • Transcription D

  • TranslationF

  • Protein synthesis G

  • Ribose B

  • Messenger RNA C

  • Transfer RNA E

  • RNA polymerase N

  • Promoter J

  • Termination signal I

  • Genetic code M

  • CodonK

  • AnticodonL

  • Genome H


Protein synthesis big picture
Protein Synthesis (Big Picture) completely

  • Cells make proteins.

  • The instructions to make a protein are on the DNA in the nucleus.

  • Ribosomes in the cytoplasm make the proteins

  • Cells MUST be able to get the instructions from the DNA inside the nucleus out to the ribosomes.

  • RNA is the messenger !!!

http://www.sciencephoto.com/images/download_lo_res.html?id=670020523


Rna structure and function
RNA Structure and Function completely

  • RNA is different from DNA in 4 ways

    • RNA sugar is ribose

    • Uracil replaces thymine as a base

    • RNA is single stranded

    • RNA is shorter than DNA


Dna vs rna
DNA vs. RNA completely

  • DNA RNA

    • Double Single

    • Deoxyribose Ribose

    • Thymine Uracil

    • Longer Shorter


Types of rna
Types of RNA completely

  • Three major types of RNA

    • Messenger RNA (mRNA) —carries instructions for making a protein from a gene in the nucleus to a ribosome in the cytoplasm

    • Ribosomal RNA (rRNA) —part of a ribosome

    • Transfer RNA (tRNA) —transfers amino acids to the ribosome to make a protein.


rRNA completely is part of the ribosome.

tRNA is folded with many nucleotide

bases. However, we emphasize the

three at the bottom.

mRNA is made from

DNA in the nucleus. It

carries the message

for making a protein

out of the nucleus to

a ribosome in the

cytoplasm


Protein synthesis
Protein Synthesis completely

  • Forming proteins based on information in DNA and carried out by RNA is called protein synthesis.

    DNA RNA protein

  • It involves two processes:

    • Transcription

    • Translation


Transcription
Transcription completely

  • Transcription —the genetic code is copied or “transcribed” onto a mRNA in the cell nucleus.

  • Three steps:

    • RNA polymerase (enzyme) binds to a specific site on a DNA molecule called a promoter. This causes DNA to unwind.

    • RNA polymerase uses the base-pairing rules to add the RNA nucleotides that match the DNA code (A/U; C/G)

    • RNA polymerase stops at a termination signal that marks the end of a gene.


Transcription1
Transcription completely

http://meyerbio1b.wikispaces.com/Transcription+and+Translation


Reading the code
Reading the Code completely

  • The code on the mRNA must next be “read” during the process of translation.

  • This genetic code tells us how a sequence of bases on a DNA molecule (or its RNA messenger) corresponds to a particular amino acid.

  • The code is read three bases at a time. Each 3 base sequence that codes for an amino acid is called a codon.


Codons in mrna
Codons completely in mRNA

  • Notice AUG is the start codon

  • UAA, UAG, and UGA are the stop codons.


Amino acids
Amino Acids completely

  • The genetic code rules are the same for nearly all living things.

  • The same codons always code for the same amino acids.

  • There are 20 different amino acids.

  • A chain of amino acids makes up a polypeptide.

  • Polypeptides join and twist to make up proteins.

  • It is tRNA and the ribosomes that assemble the proteins during translation.


  • Three bases at one end of a completely tRNA are complementary to a codon on the mRNA. They are called an anticodon.

  • The specific amino acid that the codon codes for is attached to the top of the tRNA

A U G GG A C C U

http://www.personal.psu.edu/staff/d/r/drs18/bisciImages/index.html


Translation
Translation completely

  • Translation —the making of a protein

  • Steps:

    • Initiation —ribosomal subunits, mRNA and the tRNA carrying methionine (amino acid of the start signal AUG) bind together.

    • Elongation —the tRNA carrying the amino acid specified by the next codon binds. Peptide bonds form between the amino acids beginning the chain. This continues until a termination signal is reached.

    • Termination —stop codon is reached

    • Disassembly – the ribosome complex falls apart and the peptide is released.


http://www.emc.maricopa.edu/faculty/farabee/biobk/biobookprotsyn.htmlhttp://www.emc.maricopa.edu/faculty/farabee/biobk/biobookprotsyn.html


Recap: Protein Synthesis: Big Picturehttp://www.emc.maricopa.edu/faculty/farabee/biobk/biobookprotsyn.html


The human genome
The Human Genomehttp://www.emc.maricopa.edu/faculty/farabee/biobk/biobookprotsyn.html

  • Genome —the complete genetic material contained in an individual.

  • The entire Human Genome consists of 3.2 billion base pairs. We now know the order of these base pairs and have discovered that humans have approximately 30,000 genes.

  • We now need to learn where and when human cells use each of the proteins coded for in the genome.

  • This can help diagnose, treat, and prevent many genetic disorders.


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