Genotyping transgenic mice
Download
1 / 12

Genotyping Transgenic Mice - PowerPoint PPT Presentation


  • 149 Views
  • Uploaded on

Genotyping Transgenic Mice. By: Natalie Saldana & Dr. Taha Merghoub. Introduction. Human Disease. Melanoma - serious form of skin cancer. Mouse Model – used to mimic the human disease. Transgenic Mouse. Hypothesis.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Genotyping Transgenic Mice' - fagan


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
Genotyping transgenic mice

Genotyping Transgenic Mice

By: Natalie Saldana

&

Dr. Taha Merghoub


Introduction
Introduction

Human Disease

Melanoma - serious form of skin cancer

MouseModel – used to mimic the human disease

Transgenic Mouse


Hypothesis
Hypothesis

  • The introduction of Human melanoma mutation in mice will induce this disease in mice


Why mice
Why Mice?

  • They are genetically similar to human beings

  • Affordable and easy to maintain

  • Able to reproduce within 3 weeks

  • Short life span

  • Small



Genotyping
Genotyping

  • The process of testing individuals in order to reveal any specific gene that has been inherited from that individual

  • Includes other methods like PCR, DNA sequencing, and Southern blot

  • The goal is to understand the functions and roles of genes and to test new therapies in melanoma


Materials
Materials

DNA Extraction

Mouse Tails

Qiagen DNeasy Protocol

PCR

Taq Polymerase

Buffer

MgCl2

Primers

dNTPs

DNA

H2O

Gel Electrophoresis

Agarose

TAE Buffer

Ethidium Bromide

Trays & Combs

Gel Box

Power Supply

UV Light

1 Kb Ladder

Loading Dye

Thermal Cycler (PCR Machine)



Observations results
Observations & Results

 Ink4A WT

We were successful in genotyping the mice which we will use for future experiments.

 Ink4A KO


Conclusion and future directions
Conclusion and Future Directions

  • We have identified Heterozygous and KO Ink4a mice.

  • The Ink4a mice will be crossed with NRas transgenic mice in order to obtain a mouse with melanoma.


Reference
Reference

  • http://users.ugent.be/~avierstr/principles/pcr.html

  • http://www.accessexcellence.org/RC/VL/GG/transgenic.html

  • http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/T/TransgenicAnimals.html

  • http://sunsite.berkeley.edu/PCR/whatisPCR.html

  • http://www.agrf.org.au/Default.aspx?tabid=85

  • http://ghr.nlm.nih.gov/ghr/glossary/genotyping

  • http://www.identigene.com/SWIMX/Products/mouse_genotyping.asp

  • http://en.wikipedia.org/wiki/Mouse

  • http://en.wikipedia.org/wiki/Genotyping

  • http://www.skincancer.org/melanoma/index.php


Acknowledgements
Acknowledgements

THANK YOU!

  • Dr. Taha Merghoub

  • Diana

  • Hong

  • Houghton Laboratory

  • Dr. Sat Bhattacharya

  • Harlem Children Society


ad