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รศ.ดร ดนัย บุณยเกียรติ ภาควิชาพืชสวน คณะเกษตรศาสตร์ PowerPoint PPT Presentation


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สรีรวิทยาของพืช PLANT PHYSIOLOGY. รศ.ดร ดนัย บุณยเกียรติ ภาควิชาพืชสวน คณะเกษตรศาสตร์.

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รศ.ดร ดนัย บุณยเกียรติ ภาควิชาพืชสวน คณะเกษตรศาสตร์

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PLANT PHYSIOLOGY

.


- (Fructosan)


C6H12O6 + 6O2 6CO2 + 6H2O + 1. 2. ATP NADH Respiratory Quotient (R.Q.)Respiratory Quotient 1. R.Q. 1


2. R.Q. 1 C18H3402 + 25.5 02 18CO2 + 17H2O18 R.Q. = 25.5 = 0.73. R.Q. (Amylose) (Amylopectin)


3 3

3 (b -amylase) (b -amylase) Starch Phosphorylase

1. 1, 4

2. (a-maltose)


2 (Hydrolytic Enzymes)

3. Starch Phosphorylase (Phosphorolytic Enzymes)

+ H2PO4- - 1

- 1 - ATP 1 1, 6 3 Debranching Enzyme R enzyme (Dextrinases)


(Maltase)

Maltose + H2O 2-D-glucose

1-5

Compositae 12-35


(Invertases)

(Glycolysis Fermentation)

-1- (Cytoplasm) 6 2


ATP 2 NADH 2 ATP 4 Substrate-level NADH NADH dehydrogenase ATP 2 NADH 1 ATP 8 ATP ATP 2 ATP 6 -1--6- ATP 7


1.

2. ATP

3. NADH NAD+ 3-phosphoglyceraldehyde 1,3-diphosphoglyceric acid


NADH NAD+ NADH (Ethanol) CO2 (Lactic Acid) CO2 (decarboxylated) NADH Pyruvate decarboxylase Alcohol dehydrogenase


CO2

Pyruvate kinase


Phosphofructokinase Phosphohexose isomerase Hexokinase

Rate Limiting Phosphofructokinase ATP, Phosphoenol pyruvate 3 Phospho-

glyceric acid ADP AMP Phosphofructokinase Allosteric Enzyme


(Krebs Cycle)

Citric Acid Cycle Tricarboxylic Acid Cycle TCA CO2 (Coenzyme A) CoA

(Acetyl CoA) CoA

Pyruvate decarboxylation B1 (Thiamine Vitamin B1) Prosthetic Group CO2 2 Pyruvic Acid Dehydrogenase


1. NADH FADH2 ATP 3 2 1 NADH 8 FADH2 2


Electron Transport Oxidative Phosphorylation

NADH FADH2 2 ATP NADH FADH2 Electron Transport System Cytochrome System Reduction Potential Reduction Potential


Cristae Cytochrome System (Cytochromes) 3 2 (Ubiquinone) (Cytochrome Oxidase)


Rotenone antimycin A

NADH Ubiquinone Cyt. B Cyt. C1 Cyt C.

Cyt. a + a3

(Cyt. oxidase)

Azide, CN -,CO

O2


Oxidative Phosphorylation ATP ATP ADP H2PO-4 ATP ATP FADH2 ATP NADH

ATP Gibbs Free Energy (DG) pH 7 1 1 -686,000


DG ATP 1 -7,600 / pH 7 36 ATP -273,600 -273,600/-686,000 40 60 Coupling Oxidative Phosphorylation Coupling Dinitrophenol ADP H2PO4- ATP H+ 2,4-dinitrophenol Uncoupler Oxidative Phosphorylation ATP ADP


ATP 2 Substrate Level Phosphorylation ATP ATP Chemiosmotic Hypothesis

Mitchell ATP ATP ADP H2PO4- ATPsynthase H+2 Matrix


Pentose Phosphate Pathway

Martin Gibbs Bernard Axelrod Harry Beevers 5 Pentose Phosphate Pathway PPP

PPP PPP PPP NADP NAD Glucose-6-phosphate Starch Phosphorylase Glucose-6-phosphate dehydrogenase


6-phosphogluconic acid NADP 6-phosphogluconate CO2 6-phosphogluconic acid dehydrogenase 5 Ribulose-5-Phosphate NADPH CO2 Ribulose-5-Phosphate Glucose-6-Phosphate PPP - Glucose-6-Phosphate dehydrogenase


PPP NADPH Ribulose-5-Phosphate Erythrose-4-Phosphate

Pentose phosphate Pathway

1. NADPH Reductant

2. Ribulose-5-Phosphate

3. Erythrose-4-Phosphate Shikimic acid Aromatic ring


Phosphofructokinase

3. Q10 5-25 2-2.5

4.


6


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