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รศ.ดร ดนัย บุณยเกียรติ ภาควิชาพืชสวน คณะเกษตรศาสตร์ PowerPoint PPT Presentation


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สรีรวิทยาของพืช PLANT PHYSIOLOGY. รศ.ดร ดนัย บุณยเกียรติ ภาควิชาพืชสวน คณะเกษตรศาสตร์.

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รศ.ดร ดนัย บุณยเกียรติ ภาควิชาพืชสวน คณะเกษตรศาสตร์

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PLANT PHYSIOLOGY

.


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- (Fructosan)


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C6H12O6 + 6O2 6CO2 + 6H2O + 1. 2. ATP NADH Respiratory Quotient (R.Q.)Respiratory Quotient 1. R.Q. 1


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2. R.Q. 1 C18H3402 + 25.5 02 18CO2 + 17H2O18 R.Q. = 25.5 = 0.73. R.Q. (Amylose) (Amylopectin)


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3 3

3 (b -amylase) (b -amylase) Starch Phosphorylase

1. 1, 4

2. (a-maltose)


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2 (Hydrolytic Enzymes)

3. Starch Phosphorylase (Phosphorolytic Enzymes)

+ H2PO4- - 1

- 1 - ATP 1 1, 6 3 Debranching Enzyme R enzyme (Dextrinases)


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(Maltase)

Maltose + H2O 2-D-glucose

1-5

Compositae 12-35


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(Invertases)

(Glycolysis Fermentation)

-1- (Cytoplasm) 6 2


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ATP 2 NADH 2 ATP 4 Substrate-level NADH NADH dehydrogenase ATP 2 NADH 1 ATP 8 ATP ATP 2 ATP 6 -1--6- ATP 7


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1.

2. ATP

3. NADH NAD+ 3-phosphoglyceraldehyde 1,3-diphosphoglyceric acid


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NADH NAD+ NADH (Ethanol) CO2 (Lactic Acid) CO2 (decarboxylated) NADH Pyruvate decarboxylase Alcohol dehydrogenase


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CO2

Pyruvate kinase


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Phosphofructokinase Phosphohexose isomerase Hexokinase

Rate Limiting Phosphofructokinase ATP, Phosphoenol pyruvate 3 Phospho-

glyceric acid ADP AMP Phosphofructokinase Allosteric Enzyme


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(Krebs Cycle)

Citric Acid Cycle Tricarboxylic Acid Cycle TCA CO2 (Coenzyme A) CoA

(Acetyl CoA) CoA

Pyruvate decarboxylation B1 (Thiamine Vitamin B1) Prosthetic Group CO2 2 Pyruvic Acid Dehydrogenase


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1. NADH FADH2 ATP 3 2 1 NADH 8 FADH2 2


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Electron Transport Oxidative Phosphorylation

NADH FADH2 2 ATP NADH FADH2 Electron Transport System Cytochrome System Reduction Potential Reduction Potential


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Cristae Cytochrome System (Cytochromes) 3 2 (Ubiquinone) (Cytochrome Oxidase)


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Rotenone antimycin A

NADH Ubiquinone Cyt. B Cyt. C1 Cyt C.

Cyt. a + a3

(Cyt. oxidase)

Azide, CN -,CO

O2


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Oxidative Phosphorylation ATP ATP ADP H2PO-4 ATP ATP FADH2 ATP NADH

ATP Gibbs Free Energy (DG) pH 7 1 1 -686,000


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DG ATP 1 -7,600 / pH 7 36 ATP -273,600 -273,600/-686,000 40 60 Coupling Oxidative Phosphorylation Coupling Dinitrophenol ADP H2PO4- ATP H+ 2,4-dinitrophenol Uncoupler Oxidative Phosphorylation ATP ADP


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ATP 2 Substrate Level Phosphorylation ATP ATP Chemiosmotic Hypothesis

Mitchell ATP ATP ADP H2PO4- ATPsynthase H+2 Matrix


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Pentose Phosphate Pathway

Martin Gibbs Bernard Axelrod Harry Beevers 5 Pentose Phosphate Pathway PPP

PPP PPP PPP NADP NAD Glucose-6-phosphate Starch Phosphorylase Glucose-6-phosphate dehydrogenase


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6-phosphogluconic acid NADP 6-phosphogluconate CO2 6-phosphogluconic acid dehydrogenase 5 Ribulose-5-Phosphate NADPH CO2 Ribulose-5-Phosphate Glucose-6-Phosphate PPP - Glucose-6-Phosphate dehydrogenase


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PPP NADPH Ribulose-5-Phosphate Erythrose-4-Phosphate

Pentose phosphate Pathway

1. NADPH Reductant

2. Ribulose-5-Phosphate

3. Erythrose-4-Phosphate Shikimic acid Aromatic ring


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Phosphofructokinase

3. Q10 5-25 2-2.5

4.


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6


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