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Chapters 1-4. Semester I Exam Review 2014. Which is the sugar component of a DNA nucleotide?. A. Deoxyribose B. Adenine C. Glucose D. Phosphate. A. Deoxyribose. Which substances are components of a DNA nucleotide?. A. Ribose, phosphate, and uracil

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Semester I Exam Review 2014

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Chapters 1-4

Semester I Exam Review 2014


Which is the sugar component of a DNA nucleotide?

  • A. Deoxyribose

  • B. Adenine

  • C. Glucose

  • D. Phosphate


  • A. Deoxyribose


Which substances are components of a DNA nucleotide?

  • A. Ribose, phosphate, and uracil

  • B. Phosphate, ribose, and adenine

  • C. Phosphate, deoxyribose, and uracil

  • D. Thymine, deoxyribose, and phosphate


  • D. Thymine, deoxyribose, and phosphate


The individuality of an organism is determined by the organism’s

  • Nitrogenous bases

  • Transfer RNA molecules

  • DNA nucleotide sequence

  • Amino acids


  • DNA nucleotide sequence


Which two bases are present in equal amounts in a double-stranded DNA molecule?

  • Cytosine and thymine

  • Adenine and thymine

  • Adenine and uracil

  • Cytosine and uracil


  • Adenine and thymine


During DNA replication, which enzyme is responsible for “unzipping” the DNA molecule?

  • DNA polymerase

  • DNA restriction enzymes

  • DNA ligase

  • DNA helicase


  • DNA helicase


A human DNA segment was inserted into a bacterial cell and became incorporated into the bacterial DNA. This technique is an example of

  • Cloning

  • Genetic counseling

  • Artificial selection

  • Genetic engineering


  • Genetic engineering


Which technique is used to alter bacteria in such a way that they produce human insulin?

  • Hydrolysis

  • DNA replication

  • Recombinant DNA formation

  • Genetic screening


  • Recombinant DNA formation


The insertion of a human DNA fragment into a bacterial cell might make it possible for

  • The bacterial cell to produce a human protein

  • The cloning of the human that donated that DNA fragment

  • Humans to become immune to an infection by this type of bacteria

  • The cloning of this type of bacteria


  • The bacterial cell to produce a human protein


Many diabetics are now using insulin that was made by certain bacteria. The ability of these bacteria to produce insulin was most likely the result of

  • Using radiation to trigger mutations

  • Genetic mapping of bacterial DNA to activate the gene for insulin production

  • Deleting many DNA segments from bacterial DNA

  • Inserting a portion of human DNA into the ring-shaped DNA of bacteria


  • Inserting a portion of human DNA into the ring-shaped DNA of bacteria


A small amount of DNA was taken from a fossil of a mammoth found frozen in glacial ice. Genetic technology can be used to produce a large quantity of identical DNA from this mammoth’s DNA. In this technology, the original DNA sample is used toa. stimulate differentiation in other mammoth cells.B. Act as a template for repeated replicationc. Provide fragments to replace certain human body chemicals.D. Trigger mitosis to obtain new base sequences


  • Act as a template for repeated replication


Scientists have cloned sheep, but not a human. The best explanation for this situation is that

  • There are many ethical problems involved in cloning humans

  • The technology to clone humans has not been explored

  • Human reproduction is very different from that of other mammals

  • Cloning humans would take too long.


What is the genetically engineered curdling agent in the cheese lab?

  • Rennen

  • Chymosin

  • Buttermilk

  • Whole Milk


  • Chymosin


Why did scientists produce recombinant rennin?

  • 1. Produce large quantities.

  • 2. Cheaper.


Biuret solution is an indicator for what substance?

  • Starch

  • Glucose

  • Fats

  • Proteins


  • Proteins


What color does iodine turn in the presence of starch?

  • Rusty yellow.

  • Clear

  • Purple/blue

  • Yellow/orange


Tomato plants in a garden are not growing well. The gardener hypothesizes that the soil is too acidic. To test this hypothesis accurately, the gardener could

  • Change the pH of the soil

  • Plant seeds of a different kind of plant

  • Move the tomato plants to an area with less sunlight

  • Reduce the amount of water available to the plant


  • Change the pH of the soil


To locate a specimen on a prepared slide, a student should begin with the low-power objective rather that the high-power objective because the

  • Field of vision is smaller under low power than under high power

  • Field of vision is larger under low power than under high power

  • Specimen does not need to be stained for observation under low power but must be stained for observation under high power

  • Portion of the specimen that can be observed under low power is less than the portion that can be observed under high power.


  • Field of vision is larger under low power than under high power


To measure 50 mL, which tool would be the most accurate?

  • Micropipet

  • Pipet

  • graduated cylinder

  • Balance

  • Microcentrifuge tube


  • Graduated cylinder


5 kg is equal to _______g

  • 500

  • 5000

  • 0.005

  • 0.0005


  • 5000


Which group of measurements contains only metric units?

  • 5 in, 3 kg, 40 oz

  • 5 mm, 3 g, 40 lb

  • 5 in, 3 lb, 40 mL

  • 5 mm, 3 g, 40 mL


  • 5 mm, 3 g, 40 mL


Which objective of a compound microscope would show the largest field of view?

  • 20x

  • 5x

  • 10x

  • 44x


  • 5x


Proteins are synthesized from less complex organic compounds known as:

  • Carbons

  • Starches

  • Amino acids

  • enzymes


  • Amino acids


In what organelle is DNA found?

  • Cytoplasm

  • Nucleus

  • Endoplasmic reticulum

  • Lysosome


  • nucleus


Which enzyme “pastes” rDNA fragments together?

  • Transformation enzyme

  • Restriction enzyme

  • DNA ligase

  • DNA polymerase

  • Nuclease enzyme


  • DNA ligase


A product that has entered the product pipeline

  • Is ready for clinical testing to begin.

  • Is being produced on a large scale by the manufacturing department

  • Has been determined safe and effective

  • Needs to be reviewed regularly with a comprehensive product development plan.


  • Needs to be reviewed regularly with a comprehensive product development plan


Biotechnology research labs are commonly found at all of the following facilities except

  • Universities

  • Medical clinics

  • Companies

  • Government agencies


  • Medical clinics


The primary goal in a biotechnology company is to

  • Engage in “pure science”

  • Cure all the diseases of the world

  • Publish results in scientific journals

  • Provide a product of service that is useful to society and results in earnings


  • Provide a product of service that is useful to society and results in earnings


What is meant by cell specialization?

  • Different cell lines (CHO & HeLa cells) are more suited to producing certain kinds of recombinant proteins.

  • Prokaryotic cells specialize in certain tasks and can therefore grow more rapidly than eukaryotic cells.

  • There is great cell variety in multicellular organisms in which different kinds of cells have different jobs to do.

  • All of the above


  • There is great cell variety in multicellular organisms in which different kinds of cells have different jobs to do


Which of the following is an expression of concentration units?

  • µg/µL

  • mg/mL

  • µM

  • All of these


  • all


As a solution becomes dilute

  • The proportion of solute to solvent increases

  • The proportion of solvent to solute increases

  • The normality of the solution shifts

  • Re-ionization begins to occur


  • The proportion of solvent to solute increase


Nucleotides are bonded to each other on a nucleic acid by

  • Hydrogen bonds

  • Antiparallel bonds

  • Peptide bonds

  • Phosphodiester bonds


  • Phosphodiester bonds


A circular plasmid was cut with a restriction enzyme & three bands were visualized after electrophoresis. How many cuts were made in the plasmid?

  • 1

  • 2

  • 3

  • 4


  • 3


Which agarose concentration would work best for separating very large molecules?

  • 3%

  • 2%

  • 0.8%

  • All choices would work


  • .08%


During a DNA extraction, the purpose of smashing the fruit was to

  • Break the phosphodiester bonds between nucleotides

  • Break open the nuclear membrane

  • Break apart the cellulose in the plant cells.

  • Break apart the cell membrane.


  • Break apart the cellulose surrounding plant cells.


The types of cells commonly used in biotechnology are

  • Bacteria

  • Yeast

  • Chinese hamster ovary cells

  • All of the above


  • all


Review micropipettor settings

  • P-1000

  • P-200

  • P-20


  • Metric conversions


After an enzyme cuts DNA into smaller pieces, this enzyme is responsible for gluing the new DNA pieces together

  • DNA ligase

  • DNA helicase

  • DNA emporase

  • Restriction enzymes


  • DNA ligase


What is the total magnification of an object if you are using 10X oculars and 4X objective?

  • 14X

  • 400X

  • 40X

  • 4X


  • 40X


In gel electrophoresis, what makes the DNA migrate through the gel during electrophoresis?

  • Water

  • Gravity

  • Salt

  • Electricity


  • Electricity


In PCR, the purpose for the heating cycle is to

  • Kill the bacteria.

  • Make the DNA glow.

  • Separate the DNA strands.

  • Move the DNA in the gel.


  • Separate the DNA strands


Antiparallel, with regard to DNA means that:

  • The strands cross each other

  • The strands run parallel to each other but run in opposite directions

  • The strands run the same directions and are parallel

  • Strands run at right angles to each other


  • The strands run parallel to each other but run in opposite directions


EcoRI is an example of which type of enzyme?

  • Restriction endonuclease

  • RNA polymerase

  • DNA polymerase

  • DNA ligase


  • Restriction endonuclease


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