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Chapters 1-4. Semester I Exam Review 2014. Which is the sugar component of a DNA nucleotide?. A. Deoxyribose B. Adenine C. Glucose D. Phosphate. A. Deoxyribose. Which substances are components of a DNA nucleotide?. A. Ribose, phosphate, and uracil

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Chapters 1 4

Chapters 1-4

Semester I Exam Review 2014


Which is the sugar component of a dna nucleotide
Which is the sugar component of a DNA nucleotide?

  • A. Deoxyribose

  • B. Adenine

  • C. Glucose

  • D. Phosphate


Semester i exam review 2014

  • A. Deoxyribose


Which substances are components of a dna nucleotide
Which substances are components of a DNA nucleotide?

  • A. Ribose, phosphate, and uracil

  • B. Phosphate, ribose, and adenine

  • C. Phosphate, deoxyribose, and uracil

  • D. Thymine, deoxyribose, and phosphate


Semester i exam review 2014


The individuality of an organism is determined by the organism s
The individuality of an organism is determined by the organism’s

  • Nitrogenous bases

  • Transfer RNA molecules

  • DNA nucleotide sequence

  • Amino acids



Which two bases are present in equal amounts in a double stranded dna molecule
Which two bases are present in equal amounts in a double-stranded DNA molecule?

  • Cytosine and thymine

  • Adenine and thymine

  • Adenine and uracil

  • Cytosine and uracil


Semester i exam review 2014


During dna replication which enzyme is responsible for unzipping the dna molecule
During DNA replication, which enzyme is responsible for “unzipping” the DNA molecule?

  • DNA polymerase

  • DNA restriction enzymes

  • DNA ligase

  • DNA helicase


Semester i exam review 2014

  • DNA “unzipping” the DNA molecule?helicase


Semester i exam review 2014
A human DNA segment was inserted into a bacterial cell and became incorporated into the bacterial DNA. This technique is an example of

  • Cloning

  • Genetic counseling

  • Artificial selection

  • Genetic engineering


Semester i exam review 2014

  • Genetic engineering became incorporated into the bacterial DNA. This technique is an example of


Which technique is used to alter bacteria in such a way that they produce human insulin
Which technique is used to alter bacteria in such a way that they produce human insulin?

  • Hydrolysis

  • DNA replication

  • Recombinant DNA formation

  • Genetic screening


Semester i exam review 2014


The insertion of a human dna fragment into a bacterial cell might make it possible for
The insertion of a human DNA fragment into a bacterial cell might make it possible for

  • The bacterial cell to produce a human protein

  • The cloning of the human that donated that DNA fragment

  • Humans to become immune to an infection by this type of bacteria

  • The cloning of this type of bacteria



Semester i exam review 2014

Many diabetics are now using insulin that was made by certain bacteria. The ability of these bacteria to produce insulin was most likely the result of

  • Using radiation to trigger mutations

  • Genetic mapping of bacterial DNA to activate the gene for insulin production

  • Deleting many DNA segments from bacterial DNA

  • Inserting a portion of human DNA into the ring-shaped DNA of bacteria



Semester i exam review 2014

A small amount of DNA was taken from a fossil of a mammoth found frozen in glacial ice. Genetic technology can be used to produce a large quantity of identical DNA from this mammoth’s DNA. In this technology, the original DNA sample is used toa. stimulate differentiation in other mammoth cells.B. Act as a template for repeated replicationc. Provide fragments to replace certain human body chemicals.D. Trigger mitosis to obtain new base sequences


Semester i exam review 2014

  • Act as a template for repeated replication found frozen in glacial ice. Genetic technology can be used to produce a large quantity of identical DNA from this mammoth’s DNA. In this technology, the original DNA sample is used to


Scientists have cloned sheep but not a human the best explanation for this situation is that
Scientists have cloned sheep, but not a human. The best explanation for this situation is that

  • There are many ethical problems involved in cloning humans

  • The technology to clone humans has not been explored

  • Human reproduction is very different from that of other mammals

  • Cloning humans would take too long.


What is the genetically engineered curdling agent in the cheese lab
What is the genetically engineered curdling agent in the cheese lab?

  • Rennen

  • Chymosin

  • Buttermilk

  • Whole Milk


Semester i exam review 2014


Why did scientists produce recombinant rennin
Why did scientists produce recombinant rennin? cheese lab?

  • 1. Produce large quantities.

  • 2. Cheaper.


Biuret solution is an indicator for what substance
Biuret cheese lab? solution is an indicator for what substance?

  • Starch

  • Glucose

  • Fats

  • Proteins


Semester i exam review 2014


What color does iodine turn in the presence of starch
What color does iodine turn in the presence of starch? cheese lab?

  • Rusty yellow.

  • Clear

  • Purple/blue

  • Yellow/orange


Semester i exam review 2014

Tomato plants in a garden are not growing well. The gardener hypothesizes that the soil is too acidic. To test this hypothesis accurately, the gardener could

  • Change the pH of the soil

  • Plant seeds of a different kind of plant

  • Move the tomato plants to an area with less sunlight

  • Reduce the amount of water available to the plant


Semester i exam review 2014

  • Change the pH of the soil gardener hypothesizes that the soil is too acidic. To test this hypothesis accurately, the gardener could


Semester i exam review 2014

To locate a specimen on a prepared slide, a student should begin with the low-power objective rather that the high-power objective because the

  • Field of vision is smaller under low power than under high power

  • Field of vision is larger under low power than under high power

  • Specimen does not need to be stained for observation under low power but must be stained for observation under high power

  • Portion of the specimen that can be observed under low power is less than the portion that can be observed under high power.



To measure 50 ml which tool would be the most accurate
To measure 50 powermL, which tool would be the most accurate?

  • Micropipet

  • Pipet

  • graduated cylinder

  • Balance

  • Microcentrifuge tube



5 kg is equal to g
5 powerkg is equal to _______g

  • 500

  • 5000

  • 0.005

  • 0.0005


Semester i exam review 2014


Which group of measurements contains only metric units
Which group of measurements contains only metric units? power

  • 5 in, 3 kg, 40 oz

  • 5 mm, 3 g, 40 lb

  • 5 in, 3 lb, 40 mL

  • 5 mm, 3 g, 40 mL



Which objective of a compound microscope would show the largest field of view
Which objective of a compound microscope would show the largest field of view?

  • 20x

  • 5x

  • 10x

  • 44x


Semester i exam review 2014

  • 5x largest field of view?


Proteins are synthesized from less complex organic compounds known as
Proteins are synthesized from less complex organic compounds known as:

  • Carbons

  • Starches

  • Amino acids

  • enzymes


Semester i exam review 2014


In what organelle is dna found
In what organelle is DNA found? known as:

  • Cytoplasm

  • Nucleus

  • Endoplasmic reticulum

  • Lysosome


Semester i exam review 2014


Which enzyme pastes rdna fragments together
Which enzyme “pastes” known as:rDNA fragments together?

  • Transformation enzyme

  • Restriction enzyme

  • DNA ligase

  • DNA polymerase

  • Nuclease enzyme


Semester i exam review 2014

  • DNA known as:ligase


A product that has entered the product pipeline
A product that has entered the product pipeline known as:

  • Is ready for clinical testing to begin.

  • Is being produced on a large scale by the manufacturing department

  • Has been determined safe and effective

  • Needs to be reviewed regularly with a comprehensive product development plan.



Biotechnology research labs are commonly found at all of the following facilities except
Biotechnology research labs are commonly found at all of the following facilities except

  • Universities

  • Medical clinics

  • Companies

  • Government agencies


Semester i exam review 2014


The primary goal in a biotechnology company is to
The primary goal in a biotechnology company is to following facilities except

  • Engage in “pure science”

  • Cure all the diseases of the world

  • Publish results in scientific journals

  • Provide a product of service that is useful to society and results in earnings



What is meant by cell specialization
What is meant by cell specialization? results in earnings

  • Different cell lines (CHO & HeLa cells) are more suited to producing certain kinds of recombinant proteins.

  • Prokaryotic cells specialize in certain tasks and can therefore grow more rapidly than eukaryotic cells.

  • There is great cell variety in multicellular organisms in which different kinds of cells have different jobs to do.

  • All of the above


Semester i exam review 2014


Which of the following is an expression of concentration units
Which of the following is an expression of concentration units?

  • µg/µL

  • mg/mL

  • µM

  • All of these


Semester i exam review 2014


As a solution becomes dilute
As a solution becomes dilute units?

  • The proportion of solute to solvent increases

  • The proportion of solvent to solute increases

  • The normality of the solution shifts

  • Re-ionization begins to occur



Nucleotides are bonded to each other on a nucleic acid by
Nucleotides are bonded to each other on a nucleic acid by units?

  • Hydrogen bonds

  • Antiparallel bonds

  • Peptide bonds

  • Phosphodiester bonds


Semester i exam review 2014


Semester i exam review 2014

A circular plasmid was cut with a restriction enzyme & three bands were visualized after electrophoresis. How many cuts were made in the plasmid?

  • 1

  • 2

  • 3

  • 4


Semester i exam review 2014

  • 3 bands were visualized after electrophoresis. How many cuts were made in the plasmid?


Which agarose concentration would work best for separating very large molecules
Which bands were visualized after electrophoresis. How many cuts were made in the plasmid?agarose concentration would work best for separating very large molecules?

  • 3%

  • 2%

  • 0.8%

  • All choices would work


Semester i exam review 2014

  • .08% bands were visualized after electrophoresis. How many cuts were made in the plasmid?


During a dna extraction the purpose of smashing the fruit was to
During a DNA extraction, the purpose of smashing the fruit was to

  • Break the phosphodiester bonds between nucleotides

  • Break open the nuclear membrane

  • Break apart the cellulose in the plant cells.

  • Break apart the cell membrane.



The types of cells commonly used in biotechnology are
The types of cells commonly used in biotechnology are was to

  • Bacteria

  • Yeast

  • Chinese hamster ovary cells

  • All of the above


Semester i exam review 2014


Review micropipettor settings
Review was tomicropipettor settings

  • P-1000

  • P-200

  • P-20



Semester i exam review 2014
After an enzyme cuts DNA into smaller pieces, this enzyme is responsible for gluing the new DNA pieces together

  • DNA ligase

  • DNA helicase

  • DNA emporase

  • Restriction enzymes


Semester i exam review 2014

  • DNA responsible for gluing the new DNA pieces togetherligase


What is the total magnification of an object if you are using 10x oculars and 4x objective
What is the total magnification of an object if you are using 10X oculars and 4X objective?

  • 14X

  • 400X

  • 40X

  • 4X


Semester i exam review 2014

  • 40X using 10X oculars and 4X objective?


In gel electrophoresis what makes the dna migrate through the gel during electrophoresis
In gel electrophoresis, what makes the DNA migrate through the gel during electrophoresis?

  • Water

  • Gravity

  • Salt

  • Electricity


Semester i exam review 2014


In pcr the purpose for the heating cycle is to
In PCR, the purpose for the heating cycle is to the gel during electrophoresis?

  • Kill the bacteria.

  • Make the DNA glow.

  • Separate the DNA strands.

  • Move the DNA in the gel.


Semester i exam review 2014


Antiparallel with regard to dna means that
Antiparallel the gel during electrophoresis?, with regard to DNA means that:

  • The strands cross each other

  • The strands run parallel to each other but run in opposite directions

  • The strands run the same directions and are parallel

  • Strands run at right angles to each other



Ecori is an example of which type of enzyme
EcoRI directions is an example of which type of enzyme?

  • Restriction endonuclease

  • RNA polymerase

  • DNA polymerase

  • DNA ligase


Semester i exam review 2014


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