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Chapters 1-4. Semester I Exam Review 2014. Which is the sugar component of a DNA nucleotide?. A. Deoxyribose B. Adenine C. Glucose D. Phosphate. A. Deoxyribose. Which substances are components of a DNA nucleotide?. A. Ribose, phosphate, and uracil

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Semester I Exam Review 2014

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Chapters 1 4

Chapters 1-4

Semester I Exam Review 2014


Which is the sugar component of a dna nucleotide

Which is the sugar component of a DNA nucleotide?

  • A. Deoxyribose

  • B. Adenine

  • C. Glucose

  • D. Phosphate


Semester i exam review 2014

  • A. Deoxyribose


Which substances are components of a dna nucleotide

Which substances are components of a DNA nucleotide?

  • A. Ribose, phosphate, and uracil

  • B. Phosphate, ribose, and adenine

  • C. Phosphate, deoxyribose, and uracil

  • D. Thymine, deoxyribose, and phosphate


Semester i exam review 2014

  • D. Thymine, deoxyribose, and phosphate


The individuality of an organism is determined by the organism s

The individuality of an organism is determined by the organism’s

  • Nitrogenous bases

  • Transfer RNA molecules

  • DNA nucleotide sequence

  • Amino acids


Semester i exam review 2014

  • DNA nucleotide sequence


Which two bases are present in equal amounts in a double stranded dna molecule

Which two bases are present in equal amounts in a double-stranded DNA molecule?

  • Cytosine and thymine

  • Adenine and thymine

  • Adenine and uracil

  • Cytosine and uracil


Semester i exam review 2014

  • Adenine and thymine


During dna replication which enzyme is responsible for unzipping the dna molecule

During DNA replication, which enzyme is responsible for “unzipping” the DNA molecule?

  • DNA polymerase

  • DNA restriction enzymes

  • DNA ligase

  • DNA helicase


Semester i exam review 2014

  • DNA helicase


Semester i exam review 2014

A human DNA segment was inserted into a bacterial cell and became incorporated into the bacterial DNA. This technique is an example of

  • Cloning

  • Genetic counseling

  • Artificial selection

  • Genetic engineering


Semester i exam review 2014

  • Genetic engineering


Which technique is used to alter bacteria in such a way that they produce human insulin

Which technique is used to alter bacteria in such a way that they produce human insulin?

  • Hydrolysis

  • DNA replication

  • Recombinant DNA formation

  • Genetic screening


Semester i exam review 2014

  • Recombinant DNA formation


The insertion of a human dna fragment into a bacterial cell might make it possible for

The insertion of a human DNA fragment into a bacterial cell might make it possible for

  • The bacterial cell to produce a human protein

  • The cloning of the human that donated that DNA fragment

  • Humans to become immune to an infection by this type of bacteria

  • The cloning of this type of bacteria


Semester i exam review 2014

  • The bacterial cell to produce a human protein


Semester i exam review 2014

Many diabetics are now using insulin that was made by certain bacteria. The ability of these bacteria to produce insulin was most likely the result of

  • Using radiation to trigger mutations

  • Genetic mapping of bacterial DNA to activate the gene for insulin production

  • Deleting many DNA segments from bacterial DNA

  • Inserting a portion of human DNA into the ring-shaped DNA of bacteria


Semester i exam review 2014

  • Inserting a portion of human DNA into the ring-shaped DNA of bacteria


Semester i exam review 2014

A small amount of DNA was taken from a fossil of a mammoth found frozen in glacial ice. Genetic technology can be used to produce a large quantity of identical DNA from this mammoth’s DNA. In this technology, the original DNA sample is used toa. stimulate differentiation in other mammoth cells.B. Act as a template for repeated replicationc. Provide fragments to replace certain human body chemicals.D. Trigger mitosis to obtain new base sequences


Semester i exam review 2014

  • Act as a template for repeated replication


Scientists have cloned sheep but not a human the best explanation for this situation is that

Scientists have cloned sheep, but not a human. The best explanation for this situation is that

  • There are many ethical problems involved in cloning humans

  • The technology to clone humans has not been explored

  • Human reproduction is very different from that of other mammals

  • Cloning humans would take too long.


What is the genetically engineered curdling agent in the cheese lab

What is the genetically engineered curdling agent in the cheese lab?

  • Rennen

  • Chymosin

  • Buttermilk

  • Whole Milk


Semester i exam review 2014

  • Chymosin


Why did scientists produce recombinant rennin

Why did scientists produce recombinant rennin?

  • 1. Produce large quantities.

  • 2. Cheaper.


Biuret solution is an indicator for what substance

Biuret solution is an indicator for what substance?

  • Starch

  • Glucose

  • Fats

  • Proteins


Semester i exam review 2014

  • Proteins


What color does iodine turn in the presence of starch

What color does iodine turn in the presence of starch?

  • Rusty yellow.

  • Clear

  • Purple/blue

  • Yellow/orange


Semester i exam review 2014

Tomato plants in a garden are not growing well. The gardener hypothesizes that the soil is too acidic. To test this hypothesis accurately, the gardener could

  • Change the pH of the soil

  • Plant seeds of a different kind of plant

  • Move the tomato plants to an area with less sunlight

  • Reduce the amount of water available to the plant


Semester i exam review 2014

  • Change the pH of the soil


Semester i exam review 2014

To locate a specimen on a prepared slide, a student should begin with the low-power objective rather that the high-power objective because the

  • Field of vision is smaller under low power than under high power

  • Field of vision is larger under low power than under high power

  • Specimen does not need to be stained for observation under low power but must be stained for observation under high power

  • Portion of the specimen that can be observed under low power is less than the portion that can be observed under high power.


Semester i exam review 2014

  • Field of vision is larger under low power than under high power


To measure 50 ml which tool would be the most accurate

To measure 50 mL, which tool would be the most accurate?

  • Micropipet

  • Pipet

  • graduated cylinder

  • Balance

  • Microcentrifuge tube


Semester i exam review 2014

  • Graduated cylinder


5 kg is equal to g

5 kg is equal to _______g

  • 500

  • 5000

  • 0.005

  • 0.0005


Semester i exam review 2014

  • 5000


Which group of measurements contains only metric units

Which group of measurements contains only metric units?

  • 5 in, 3 kg, 40 oz

  • 5 mm, 3 g, 40 lb

  • 5 in, 3 lb, 40 mL

  • 5 mm, 3 g, 40 mL


Semester i exam review 2014

  • 5 mm, 3 g, 40 mL


Which objective of a compound microscope would show the largest field of view

Which objective of a compound microscope would show the largest field of view?

  • 20x

  • 5x

  • 10x

  • 44x


Semester i exam review 2014

  • 5x


Proteins are synthesized from less complex organic compounds known as

Proteins are synthesized from less complex organic compounds known as:

  • Carbons

  • Starches

  • Amino acids

  • enzymes


Semester i exam review 2014

  • Amino acids


In what organelle is dna found

In what organelle is DNA found?

  • Cytoplasm

  • Nucleus

  • Endoplasmic reticulum

  • Lysosome


Semester i exam review 2014

  • nucleus


Which enzyme pastes rdna fragments together

Which enzyme “pastes” rDNA fragments together?

  • Transformation enzyme

  • Restriction enzyme

  • DNA ligase

  • DNA polymerase

  • Nuclease enzyme


Semester i exam review 2014

  • DNA ligase


A product that has entered the product pipeline

A product that has entered the product pipeline

  • Is ready for clinical testing to begin.

  • Is being produced on a large scale by the manufacturing department

  • Has been determined safe and effective

  • Needs to be reviewed regularly with a comprehensive product development plan.


Semester i exam review 2014

  • Needs to be reviewed regularly with a comprehensive product development plan


Biotechnology research labs are commonly found at all of the following facilities except

Biotechnology research labs are commonly found at all of the following facilities except

  • Universities

  • Medical clinics

  • Companies

  • Government agencies


Semester i exam review 2014

  • Medical clinics


The primary goal in a biotechnology company is to

The primary goal in a biotechnology company is to

  • Engage in “pure science”

  • Cure all the diseases of the world

  • Publish results in scientific journals

  • Provide a product of service that is useful to society and results in earnings


Semester i exam review 2014

  • Provide a product of service that is useful to society and results in earnings


What is meant by cell specialization

What is meant by cell specialization?

  • Different cell lines (CHO & HeLa cells) are more suited to producing certain kinds of recombinant proteins.

  • Prokaryotic cells specialize in certain tasks and can therefore grow more rapidly than eukaryotic cells.

  • There is great cell variety in multicellular organisms in which different kinds of cells have different jobs to do.

  • All of the above


Semester i exam review 2014

  • There is great cell variety in multicellular organisms in which different kinds of cells have different jobs to do


Which of the following is an expression of concentration units

Which of the following is an expression of concentration units?

  • µg/µL

  • mg/mL

  • µM

  • All of these


Semester i exam review 2014

  • all


As a solution becomes dilute

As a solution becomes dilute

  • The proportion of solute to solvent increases

  • The proportion of solvent to solute increases

  • The normality of the solution shifts

  • Re-ionization begins to occur


Semester i exam review 2014

  • The proportion of solvent to solute increase


Nucleotides are bonded to each other on a nucleic acid by

Nucleotides are bonded to each other on a nucleic acid by

  • Hydrogen bonds

  • Antiparallel bonds

  • Peptide bonds

  • Phosphodiester bonds


Semester i exam review 2014

  • Phosphodiester bonds


Semester i exam review 2014

A circular plasmid was cut with a restriction enzyme & three bands were visualized after electrophoresis. How many cuts were made in the plasmid?

  • 1

  • 2

  • 3

  • 4


Semester i exam review 2014

  • 3


Which agarose concentration would work best for separating very large molecules

Which agarose concentration would work best for separating very large molecules?

  • 3%

  • 2%

  • 0.8%

  • All choices would work


Semester i exam review 2014

  • .08%


During a dna extraction the purpose of smashing the fruit was to

During a DNA extraction, the purpose of smashing the fruit was to

  • Break the phosphodiester bonds between nucleotides

  • Break open the nuclear membrane

  • Break apart the cellulose in the plant cells.

  • Break apart the cell membrane.


Semester i exam review 2014

  • Break apart the cellulose surrounding plant cells.


The types of cells commonly used in biotechnology are

The types of cells commonly used in biotechnology are

  • Bacteria

  • Yeast

  • Chinese hamster ovary cells

  • All of the above


Semester i exam review 2014

  • all


Review micropipettor settings

Review micropipettor settings

  • P-1000

  • P-200

  • P-20


Semester i exam review 2014

  • Metric conversions


Semester i exam review 2014

After an enzyme cuts DNA into smaller pieces, this enzyme is responsible for gluing the new DNA pieces together

  • DNA ligase

  • DNA helicase

  • DNA emporase

  • Restriction enzymes


Semester i exam review 2014

  • DNA ligase


What is the total magnification of an object if you are using 10x oculars and 4x objective

What is the total magnification of an object if you are using 10X oculars and 4X objective?

  • 14X

  • 400X

  • 40X

  • 4X


Semester i exam review 2014

  • 40X


In gel electrophoresis what makes the dna migrate through the gel during electrophoresis

In gel electrophoresis, what makes the DNA migrate through the gel during electrophoresis?

  • Water

  • Gravity

  • Salt

  • Electricity


Semester i exam review 2014

  • Electricity


In pcr the purpose for the heating cycle is to

In PCR, the purpose for the heating cycle is to

  • Kill the bacteria.

  • Make the DNA glow.

  • Separate the DNA strands.

  • Move the DNA in the gel.


Semester i exam review 2014

  • Separate the DNA strands


Antiparallel with regard to dna means that

Antiparallel, with regard to DNA means that:

  • The strands cross each other

  • The strands run parallel to each other but run in opposite directions

  • The strands run the same directions and are parallel

  • Strands run at right angles to each other


Semester i exam review 2014

  • The strands run parallel to each other but run in opposite directions


Ecori is an example of which type of enzyme

EcoRI is an example of which type of enzyme?

  • Restriction endonuclease

  • RNA polymerase

  • DNA polymerase

  • DNA ligase


Semester i exam review 2014

  • Restriction endonuclease


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