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Using Your Staining Antibodies with BD CompBeads PowerPoint PPT Presentation


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Using Your Staining Antibodies with BD CompBeads. Verify Ab has Kappa light chain. Match Ab to species. Mouse - use BD#552843. Rat - use BD#552844. Rat/Hamster - use BD#552845. Single stain beads as if 1x10 6 cells. Use amount to match or exceed brightness of stained cells.

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Using Your Staining Antibodies with BD CompBeads

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Using your staining antibodies with bd compbeads l.jpg

Using Your Staining Antibodies with BD CompBeads

Verify Ab has Kappa light chain

Match Ab to species

Mouse - use BD#552843

Rat - use BD#552844

Rat/Hamster - use BD#552845

Single stain beads as if 1x106 cells

Use amount to match or exceed brightness of stained cells

Run as compensation sample

Use validated instrument settings

Fit beads on scatter plot

Gate on singlet beads

Acquire sufficient events

Flow Core= 15,000 all

M. Roederer= 50,000 gated

Calculate compensation

060320 / EVC-FCC / Suzanne Mertens / [email protected]


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