Genetic engineering
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Genetic Engineering. Selective breeding – controlled breeding for desired characteristics; takes advantage of naturally occurring genetic variation – Fig. 13 – 1 What are some organisms that are good examples of selective breeding?

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Genetic Engineering

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Genetic engineering

Genetic Engineering


Genetic engineering1

Selective breeding – controlled breeding for desired characteristics; takes advantage of naturally occurring genetic variation – Fig. 13 – 1

What are some organisms that are good examples of selective breeding?

1. hybridization – breeding technique that involves crossing dissimilar individuals to bring together the best traits of both organisms

2. inbreeding – mating between organisms that are genetically similar: promotes preservation of desired characteristics; decreases genetic variation

Genetic Engineering


Hybridization

Hybridization

X


Inbreeding

Inbreeding


Genetic engineering2

Increasing genetic variation can be accomplished by inducing mutations

1. Bacteria – radiation or chemicals can cause “beneficial mutations” – Fig. 13-3

2. Plants – chemicals that prevent chromosomal separation lead to polyploidy (What advantages do these plants have?)

3. Induced mutations can also be used to study the function of proteins

Genetic Engineering


Manipulating dna

How do scientists change/manipulate DNA?

1. DNA extraction – remove DNA from the cell

2. DNA cutting – restriction enzymes “cut” DNA at very specific nucleotide sequences

Fig. 13-5

Manipulating DNA

Restriction Enzyme http://www.youtube.com/watch?v=yc-s-WojU5Y&feature=related


Manipulating dna1

How do scientists change/manipulate DNA?

3. DNA separating – gel electrophoresis uses an electrical current to separate fragments of DNA based on size – Fig. 13-6

Manipulating DNA

Gel Electrophoresis

http://www.sumanasinc.com/webcontent/animations/content/gelelectrophoresis.html


Using the dna sequence

Reading the sequence allows the study of specific genes – Fig. 13 -7 (Sanger Sequence)

1. Start with a single strand of DNA with unknown sequence

2. Mix this strand with DNA polymerase and a lot of nucleotides; some of the nucleotides are “tagged” with dye

3. each time a “tagged” nucleotide is added replication stops

4. Using gel electrophoresis complementary DNA strand is “read”

Using the DNA sequence


Using the dna sequence1

Sanger Sequencing Videos

http://www.youtube.com/watch?v=aPN8LP4YxPo&feature=related

http://www.youtube.com/watch?v=6ldtdWjDwes&feature=related

Using the DNA sequence


Pcr polymerase chain reaction

PCR – Polymerase Chain Reaction

A technique by which any segment of DNA can be quickly amplified (copied many times) – Fig. 13-8

1. DNA is separated and added to a mixture of nucleotides and enzymes

2. New complementary strands are made

3. Cycle is repeated many times


Genetic engineering

PCR


Genetic engineering

PCR

http://www.youtube.com/watch?v=JRAA4C2OPwg&feature=related PCR Animation

PCR Song http://www.youtube.com/watch?v=x5yPkxCLads


Cell transformation taking up foreign dna

Cell Transformation – taking up foreign DNA

Some bacteria can take up naked DNA from the surroundings.

Plasmid – a small, circular, self-replicating DNA molecule separate from the bacterial chromosome

Assimilated foreign DNA is taken up by the plasmid

Offspring of the recipient bacterium will carry a new combination of genes

Genetic marker – a gene that produces a known protein that can be used to “mark” bacteria that have taken up foreign DNA


Cell transformation

Cell Transformation


Cell transformation1

Cell Transformation

Plant Transformation – Fig. 13-10

Plasmids that normally infect plant cells can be used as carriers of foreign DNA

Whatever gene is taken up is then expressed by the plant cell

What are some advantages and disadvantages of this technology?


Cell transformation2

The bacterium can be used to introduce foreign DNA into plant cells. If the transformation is successful, the DNA will be integrated into one of the cell’s chromosomes.

Cell Transformation


Cell transformation3

Cell Transformation

Animal Transformation – Fig. 13-11

Eggs cells are large enough to take up foreign DNA

DNA is inserted manually and enzymes normally present in the cell to repair DNA help to insert the foreign DNA

Technique can be sued to study specific functions of a gene


Applications of genetic engineering

Applications of Genetic Engineering

Transgenic organisms contain genes from another species; possible because of the universal nature of the genetic code – Fig. 13-12

Microorganisms – easy to grow, divide rapidly, can be used to produce human proteins

Animals can be used to improve food supply, or to study effect of human diseases

Plants – genes can be implanted that provide plants with natural insecticides, or resistance to various chemicals


Transgeneic organisms

This transgenic tobacco plant, which glows in the dark, was grown from a tobacco cell transformed with the firefly luciferase gene. The plant illustrates how DNA from one organism contains information that can specify traits in another organism.

Transgeneic Organisms


Applications of genetic engineering1

Applications of Genetic Engineering

Cloning – A clone is a lineage of genetically identical individuals or cells

Basic cloning technique – Fig. 13-13 KNOW!!!


Transgenic organisms

In early 1997, Dolly made headlines as the first clone of an adult mammal.

Transgenic Organisms


Transgenic organisms1

Why did Dolly not look like her foster mother?

Transgenic Organisms


Transgenic organisms2

The adult sheep is Dolly. The lamb is Dolly’s first offspring., called Bonnie. The fact that dolly was cloned did ot affect her ability to produce a live offspring. Why might it be important for cloned animals to be able to reproduce?

Transgenic Organisms


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