The effect of centrophenoxine on parkinson s disease
This presentation is the property of its rightful owner.
Sponsored Links
1 / 18

The Effect of Centrophenoxine on Parkinson’s Disease PowerPoint PPT Presentation


  • 108 Views
  • Uploaded on
  • Presentation posted in: General

The Effect of Centrophenoxine on Parkinson’s Disease . Victoria Wei. Need. Taken from Rajput AH, Offord KP, Beard CM, Kurland LT. Epidemiology of parkinsonism: incidence, classification, and mortality. Ann Neurol. 1984;16:278-282. .

Download Presentation

The Effect of Centrophenoxine on Parkinson’s Disease

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


The effect of centrophenoxine on parkinson s disease

The Effect of Centrophenoxine on Parkinson’s Disease

Victoria Wei


The effect of centrophenoxine on parkinson s disease

Need

Taken from Rajput AH, Offord KP, Beard CM, Kurland LT. Epidemiology of parkinsonism: incidence, classification, and mortality. Ann Neurol. 1984;16:278-282.

Figure 1 The amount of Parkinson’s disease cases per 100,000 people in the United States as age increases


Knowledge base

Knowledge Base

  • Parkinson’s disease is a brain disorder involving the nerves.

Figure 2 The effects of Parkinson’s disease

http://www.spinstudios.co.uk/sa/pa3.jpg


Knowledge base1

Knowledge Base

http://www.wormatlas.org/handbook/fig.s/IntroFIG6.jpg

Figure 3 The life cycle of C. elegans


Knowledge base2

Knowledge Base

http://www.innovitaresearch.org/news/res/06042501_01.jpg

Figure 4 Lipofuscin in neurons of the human brain.


Knowledge base3

Knowledge Base

http://resources.metapress.com/pdf-preview.axd?code=w35465l87q324635&size=largest

Figure 5 structure of centrophenoxide and the products of the hydrolysis- which are dimethylethanolamine (DMAE) and p-chlorophenoxyacetic acid (PCPA)


Literature review

Literature Review

  • Caldwin, et. al. (2008)

Figure 6

Figure 7

Both images taken from Caldwin, Guy A.; K.A. Caldwell. “Traversing a wormhole to Combat Parkinson’s disease.” Disease Models and Mechanisms. Volume 1. pp.000-000. 2008.


Literature review1

Literature Review

  • Gerstbrein, et. al. (2008)

  • Lipofuscin is a marker of lifespan in C. elegans

Figure 9 Auto fluorescent lipofuscin pigments present in Day 7 and Day 12 C. elegans.

Gerstbrein, Beate; G. Stamatas; N. Kollias; M. Driscoll. “In vivspectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction in Caenorhabditis elegans.


Literature review2

Literature Review

  • Nass, et. al., (2002)

  • GFP is a usable method of observing the dopamine neurons in C. elegans.

Dopamine neurons are unaffected

Dopamine neurons are progressively dying

Figure 10 Degeneration of dopamine neurons

http://docs.google.com/gview?a=v&q=cache%3AtdtxA_lhng0J%3Aweb.mac.com%2Frdblakely%2FBlakelyLab%2FBlakely_Lab_News_files%2FWorm%2520research%2520yields%2520powerful%2520model%2520for%2520Parkinson%27s%2520disease%25203.1.02.pdf+c+elegans+parkinson%27s+filetype%3Apdf&hl=en&gl=us


Literature review3

Literature Review

  • Application of centrophenoxine to the C. elegans decreased the rate of lipofuscin accumulation by an average of 41.3%. (Shulkin, et. al., 1978)

Figure 10 Table displaying effect of centrophenoxine on lipofuscin in C. elegans

Shulkin, D.J.; B.M. Zuckerman. “Spectrofluorometric analysis of the effect of centrophenoxine on lipofuscin accumulation in the nematode C. elegans.” Age. Volume 5. Pp. 50-53. 1982.


Purpose

Purpose

  • The purpose of the experiment is to observe the effects of centrophenoxine on Parkinson’s disease in C. elegans.

Hypothesis

  • Null- the symptoms of Parkinson’s disease will remain the same with or without the application of centrophenoxine.

  • Alternate- the symptoms of Parkinson’s disease will lessen with the application of centrophenoxine.


Methodology

The Effects of Centrophenoxineon Parkinson’s disease in Caenorhabditiselegans

Methodology

Caenorhabditis elegansham-1(ot339) obtained from the Caenorhabditis Genetics Center (CGC)

N=100

Given 6.8 mMcentrophenoxine:

N=50

No centrophenoxine is applied:

N=50

C. elegans will be cultured in the Nematode Growth Media at 20 °C , and fed Escherichia coli(op50). 6.8 mMcentrophenoxine will be applied to the NGM where the C. elegans are cultured and observed throughout lifespan.

Qualitative data will be collected on how strong both the GFP and lipofuscin fluorescence is-using a scale that ranges the strength of fluorescence.

Statistical analysis with SPSS


Protocol

Protocol

  • C. elegans are grown in petri dishes at 20°C with NGM and fed U.V. killed E. coli.

  • Both Ampicillin and 5-Fluoro-2′-deoxyuridin (FUDR) are added to the plates

Picture by author

Figure 11 Culturing the C. elegans in a petri dish


Protocol1

Protocol

E.coli + NGM with centrophenoxine + Amp + FUDR using the DAPI and GFP filter to observe amount of lipofuscin and GFP fluorescence present in both C. elegans ham-1(ot339)groups


Protocol2

Protocol

Sutphin, George; M. Kaeberlein. “Measuring Caenorhabditis elegans Life Span on Solid Media” JOVE. 2009.

Figure 12 Age synchronization of C. elegans


Budget

Budget


Do ability

Do-ability

Available for Purchase:

  • ham-1(ot339) C. elegans strain from CGC

  • DAPI and Sodium Azide from Sigma

  • NGM and OP50 E.coli from Carolina Biological

  • Centrophenoxinefrom Science Lab.com

    Equipment already Acquired:

  • DAPI filter, GFP filter, fluorescent microscope, UV lights


Bibliography

Bibliography

  •  "About Parkinson Disease." National Parkinson Foundation. <”http://www.parkinson.org/Page.aspx?pid=225”>. 1996-2007.

  • Braungart, Evelyn; Gerlach, Manfred; Riederer; Peter, Baumeister, Ralf; and Hoener, Marius C. “Caenorhabditis elegans MPP+ Model of Parkinson’s Disease for High-throughout Drug Screening.” Neurodegenerative Disease. 2004. Volume 1: pgs 175-183.

  • Caldwin, Guy A.; K.A. Caldwell. “Traversing a wormhole to Combat Parkinson’s disease.” Disease Models and Mechanisms. Volume 1. pp.000-000. 2008.

  • Colleta, Susan. Introduction to C. elegans. Waksman Student Scholars. <http://avery.rutgers.edu/WSSP/StudentScholars/project/introduction/worms.html>. 2009

  • Gerstbrein, Beate; G. Stamatas; N. Kollias; M. Driscoll. “In vivspectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction in Caenorhabditis elegans.

  • Hall, D. H.; Z. F. Altun. “C. elegans Atlas.” Genetics Research,90 , pp 375-376. 2008.

  • Hunt, Sara S. The Aging Process. Washington D.C. April 2004.

  • Kenyon, Cynthia. “Environmental Factors and Gene Activities That Influence Life Span” C. elegans II. Cold Spring Harbor Press. 1997.

  • Kisiel, Marion J.; B. Zuckerman. “Effects of Centrophenoxine on the Nematode CaenorhabditisBriggsae” Age. Volume 1. Pp.17-20. January 1978.

  • Mc Naught, KS; P. Jenner. “Proteasomal function is impaired in substantianigra in Parkinson's disease “ Neuroscience Letters. Volume 297. pp. 191-194. 2001.

  • Nass, Richard; R. Blakely; D. Miller. “Worm yields powerful research model for Parkinson’s disease.” The Reporter. 2002.

  • O'Riordan ; A.M. Burnell. Intermediary metabolism in the dauer larva. II. The glyoxylate cycle and fatty acid oxidation. Comp. Biochem. Physiol. Volume 95. pp. 125-130. 1990.

  • Rajput AH, Offord KP, Beard CM, Kurland LT. Epidemiology of parkinsonism: incidence, classification, and mortality. Ann Neurol. 1984;16:278-282.

  • Schneider, Howard F.; C. Nandy. “Effects of Centrophenoxine on Lipofuscin Formation in Neuroblastoma Cells in Culture” Journal of Gerontology. Volume 32. Pp. 132-139. 1997.

  • Shulkin, D.J.; B.M. Zuckerman. “Spectrofluorometric analysis of the effect of centrophenoxine on lipofuscin accumulation in the nematode C. elegans.” Age. Volume 5. Pp. 50-53. 1982.

  • Sutphin, George; M. Kaeberlein. “Measuring Caenorhabditis elegans Life Span on Solid Media” JOVE. 2009.

  • “What is Parkinson’s?” American Parkinson Disease Association West Coast Office. <“http://www.apdawest.org/WhatIsParkinsons.html#2”>. 2009.


  • Login