Cell characterization measurement of viability
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Cell Characterization & Measurement of Viability . Dr Alok Tripathi [email protected] +919915546129 Department of Biotechnology . 6x10 6. Note the lag phase before the cells start to grow, the log

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Cell Characterization & Measurement of Viability

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Cell characterization measurement of viability

Cell Characterization & Measurement of Viability

Dr Alok Tripathi

[email protected]

+919915546129

Department of Biotechnology


Cell characterization measurement of viability

6x106

Note the lag phase before the cells start to grow, the log

phase of growth, and the beginning of the plateau phase when contact-inhibited cells will cease growing. The population doubling time should be calculated during log phase growth and is 20 hr for this cell line (RL-65)

1x106

1x105

1x104


Using the hemacytometer

Using the Hemacytometer

  • Materials

  • 1. Trypsinized cell cultures

  • 2. Improved Neubauerhemacytometer with coverslip

  • 3. Tally counter

  • 4. 0.4% trypan blue in PBS

  • 5. Pasteur pipettes

  • 6. Microscope


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