Cell characterization measurement of viability
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Cell Characterization & Measurement of Viability PowerPoint PPT Presentation


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Cell Characterization & Measurement of Viability . Dr Alok Tripathi [email protected] +919915546129 Department of Biotechnology . 6x10 6. Note the lag phase before the cells start to grow, the log

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Cell Characterization & Measurement of Viability

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Cell Characterization & Measurement of Viability

Dr Alok Tripathi

[email protected]

+919915546129

Department of Biotechnology


6x106

Note the lag phase before the cells start to grow, the log

phase of growth, and the beginning of the plateau phase when contact-inhibited cells will cease growing. The population doubling time should be calculated during log phase growth and is 20 hr for this cell line (RL-65)

1x106

1x105

1x104


Using the Hemacytometer

  • Materials

  • 1. Trypsinized cell cultures

  • 2. Improved Neubauerhemacytometer with coverslip

  • 3. Tally counter

  • 4. 0.4% trypan blue in PBS

  • 5. Pasteur pipettes

  • 6. Microscope


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