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Depression and PER2. Andie Held. http:// www.genengnews.com /gen-news-highlights/genetic-link-for-depression-furthered-by-analysis-of-54-studies/81244463/. What gets you down in the dumps?. http:// www.desibucket.com / db /02/25158/25158.jpg. What is depression? .

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depression and per2

Depression and PER2

Andie Held

http://www.genengnews.com/gen-news-highlights/genetic-link-for-depression-furthered-by-analysis-of-54-studies/81244463/

what gets you down in the dumps
What gets you down in the dumps?

http://www.desibucket.com/db/02/25158/25158.jpg

what is depression
What is depression?

Low mood and sadness that interferes with daily life

Affects 6.7% of the adult population in the U.S.

Attributable to genetic, biological, environmental, and psychological factors

http://www.steveseay.com/depression-symptoms-major-depressive-disorder/

per2 who
PER2, Who?

Protein from Pfam

Light reception

Regulation of circadian rhythm

Always found C-terminal to PAS

http://www.huffingtonpost.com/dr-susan-taylor/can-race-possibly-influen_b_1914649.html; http://2.bp.blogspot.com/-Ph9jPyaTtSs/UGiFJIAiECI/AAAAAAAAAlc/7FGx6NV4LSc/s1600/Day_And_Night_for_draw.jpeg; http://assets.openstudy.com/updates/attachments/513c2f1ae4b01c4790d24e4d-easyuser-1362926137205-dopamine_chemical_structure.png

how well conserved is per2
How well conserved is PER2?

% Identity

1255

Human

91%

1310

Chimp

93%

Rhesus Monkey

1266

Dog

73%

1265

73%

Cattle

1291

77%

Mouse

1257

Rat

77%

1257

99%

Gorilla

1344

51%

Zebrafish

1404

how well conserved is per21
How well conserved is PER2?

Phylogram from Clustal Omega

are there natural remedies for depression
Are there natural remedies for depression?

Valerian Root

http://www.herbalextractsplus.com/images/herbs/valerian-isp.jpg

primary goal
Primary goal

The primary goal of this study is to determine how the light/dark cycle affects dopamine receptor expression as well as how natural products such as valerian affect gene expression

which model organisms should we use to study depression
Which model organisms should we use to study depression?

77%

Standard circadian model

72%

51%

Similarity percents determined by BLAST

http://1.bp.blogspot.com/_FY8xQMCUsY8/S_MFEQzaSNI/AAAAAAAAACo/aC1euzhdlfU/s1600/group%2520diverse%2520happy%2520people%2520black%2520white%2520brown%2520smile%2520old%2520young%2520teen.jpg; http://www.renalgenes.org/zebrafish.html; http://www.ryanphotographic.com/characidae.htm

aim 1 how well conserved is per2 in blind cavefish
Aim 1: How well conserved is PER2 in blind cavefish?

PAS_11

Alignments courtesy of Clustal Omega

slide11
Aim 2: How are PER2 and D2 dopamine receptors expressed in light/dark environments in species with different eye structures?
slide12

X

RNA-seq

RNA-seq

PER2

PER2

PER2

PER2

Dop

Dop

Dop

Dop

slide14

WT

PER2 MUTANT

Label free quantification;

Mass spectrometry

Label free quantification;

Mass spectrometry

Semi relief of stillness & hiding

Normal Behavior

Relaxed

behavior

Stillness

and hiding

DopR

DopR

DopR

DopR

homologs and flowchart picture references
Homologs and flowchart picture references
  • Proteins on slide 6 are curtsey of Homologene
  • Sun http://www.clipartbest.com/cliparts/9cz/EMK/9czEMKnKi.gif
  • Moon http://bestclipartblog.com/clipart-pics/moon-clip-art-11.gif
  • Fish http://www.clker.com/cliparts/r/C/E/Y/M/x/simple-fish-md.png
  • Fish Food http://i.walmartimages.com/i/p/00/04/67/98/77/0004679877035_500X500.jpg
  • Valerian root http://www.herbalextractsplus.com/images/herbs/valerian-isp.jpg
  • Brain http://static.freepik.com/free-photo/cartoon-brain-outline-clip-art_411969.jpg
future directions
Future Directions

Genetics:

  • Further investigate the role of dopamine in depression and other natural products that regulate it
  • Duplicate specific aim experiments with the addition of depression trigger events

Social Work:

  • Educate the public to help fight the mental illness stigma
  • Advocate for equal access to mental health care
resources
Resources

http://www.dbsalliance.org/site/PageServer?pagename=home; http://www.nami.org/Content/NavigationMenu/Campaign_for_the_Mind_of_America/Mental_Illness_Awareness_Week/MIAW-sticker-circle-2.5.jpg; http://www.suicidepreventionlifeline.org/

questions
Questions?

http://chibird.com/post/74679635068/a-little-positive-bunny-post-to-brighten-up-your

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