Depression and per2
This presentation is the property of its rightful owner.
Sponsored Links
1 / 18

Depression and PER2 PowerPoint PPT Presentation


  • 74 Views
  • Uploaded on
  • Presentation posted in: General

Depression and PER2. Andie Held. http:// www.genengnews.com /gen-news-highlights/genetic-link-for-depression-furthered-by-analysis-of-54-studies/81244463/. What gets you down in the dumps?. http:// www.desibucket.com / db /02/25158/25158.jpg. What is depression? .

Download Presentation

Depression and PER2

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Depression and per2

Depression and PER2

Andie Held

http://www.genengnews.com/gen-news-highlights/genetic-link-for-depression-furthered-by-analysis-of-54-studies/81244463/


What gets you down in the dumps

What gets you down in the dumps?

http://www.desibucket.com/db/02/25158/25158.jpg


What is depression

What is depression?

Low mood and sadness that interferes with daily life

Affects 6.7% of the adult population in the U.S.

Attributable to genetic, biological, environmental, and psychological factors

http://www.steveseay.com/depression-symptoms-major-depressive-disorder/


Per2 who

PER2, Who?

Protein from Pfam

Light reception

Regulation of circadian rhythm

Always found C-terminal to PAS

http://www.huffingtonpost.com/dr-susan-taylor/can-race-possibly-influen_b_1914649.html; http://2.bp.blogspot.com/-Ph9jPyaTtSs/UGiFJIAiECI/AAAAAAAAAlc/7FGx6NV4LSc/s1600/Day_And_Night_for_draw.jpeg; http://assets.openstudy.com/updates/attachments/513c2f1ae4b01c4790d24e4d-easyuser-1362926137205-dopamine_chemical_structure.png


How well conserved is per2

How well conserved is PER2?

% Identity

1255

Human

91%

1310

Chimp

93%

Rhesus Monkey

1266

Dog

73%

1265

73%

Cattle

1291

77%

Mouse

1257

Rat

77%

1257

99%

Gorilla

1344

51%

Zebrafish

1404


How well conserved is per21

How well conserved is PER2?

Phylogram from Clustal Omega


Are there natural remedies for depression

Are there natural remedies for depression?

Valerian Root

http://www.herbalextractsplus.com/images/herbs/valerian-isp.jpg


Primary goal

Primary goal

The primary goal of this study is to determine how the light/dark cycle affects dopamine receptor expression as well as how natural products such as valerian affect gene expression


Which model organisms should we use to study depression

Which model organisms should we use to study depression?

77%

Standard circadian model

72%

51%

Similarity percents determined by BLAST

http://1.bp.blogspot.com/_FY8xQMCUsY8/S_MFEQzaSNI/AAAAAAAAACo/aC1euzhdlfU/s1600/group%2520diverse%2520happy%2520people%2520black%2520white%2520brown%2520smile%2520old%2520young%2520teen.jpg; http://www.renalgenes.org/zebrafish.html; http://www.ryanphotographic.com/characidae.htm


Aim 1 how well conserved is per2 in blind cavefish

Aim 1: How well conserved is PER2 in blind cavefish?

PAS_11

Alignments courtesy of Clustal Omega


Depression and per2

Aim 2: How are PER2 and D2 dopamine receptors expressed in light/dark environments in species with different eye structures?


Depression and per2

X

RNA-seq

RNA-seq

PER2

PER2

PER2

PER2

Dop

Dop

Dop

Dop


Depression and per2

Aim 3: How does valerian root affect PER2 protein expression?


Depression and per2

WT

PER2 MUTANT

Label free quantification;

Mass spectrometry

Label free quantification;

Mass spectrometry

Semi relief of stillness & hiding

Normal Behavior

Relaxed

behavior

Stillness

and hiding

DopR

DopR

DopR

DopR


Homologs and flowchart picture references

Homologs and flowchart picture references

  • Proteins on slide 6 are curtsey of Homologene

  • Sun http://www.clipartbest.com/cliparts/9cz/EMK/9czEMKnKi.gif

  • Moon http://bestclipartblog.com/clipart-pics/moon-clip-art-11.gif

  • Fish http://www.clker.com/cliparts/r/C/E/Y/M/x/simple-fish-md.png

  • Fish Food http://i.walmartimages.com/i/p/00/04/67/98/77/0004679877035_500X500.jpg

  • Valerian root http://www.herbalextractsplus.com/images/herbs/valerian-isp.jpg

  • Brain http://static.freepik.com/free-photo/cartoon-brain-outline-clip-art_411969.jpg


Future directions

Future Directions

Genetics:

  • Further investigate the role of dopamine in depression and other natural products that regulate it

  • Duplicate specific aim experiments with the addition of depression trigger events

    Social Work:

  • Educate the public to help fight the mental illness stigma

  • Advocate for equal access to mental health care


Resources

Resources

http://www.dbsalliance.org/site/PageServer?pagename=home; http://www.nami.org/Content/NavigationMenu/Campaign_for_the_Mind_of_America/Mental_Illness_Awareness_Week/MIAW-sticker-circle-2.5.jpg; http://www.suicidepreventionlifeline.org/


Questions

Questions?

http://chibird.com/post/74679635068/a-little-positive-bunny-post-to-brighten-up-your


  • Login