Download

Aerobic Non-Spore Forming Gram-Positive Bacilli






Advertisement
/ 32 []
Download Presentation
Comments
elspeth
From:
|  
(126) |   (0) |   (0)
Views: 160 | Added:
Rate Presentation: 0 0
Description:
Aerobic Non-Spore Forming Gram-Positive Bacilli. Corynebacterium. Gram Positive Bacilli. Gram positive rods. Spore forming. Non spore forming. Aerobic. Anaerobic. Corynebacterium. Clostridium spp. Bacillus spp. Species of Corynebacteria. Corynebacterium diphtheriae.
Aerobic Non-Spore Forming Gram-Positive Bacilli

An Image/Link below is provided (as is) to

Download Policy: Content on the Website is provided to you AS IS for your information and personal use only and may not be sold or licensed nor shared on other sites. SlideServe reserves the right to change this policy at anytime. While downloading, If for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.











- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -




Slide 1

Aerobic Non-Spore Forming Gram-Positive Bacilli

Corynebacterium

Slide 2

Gram Positive Bacilli

Gram positive rods

Spore forming

Non spore forming

Aerobic

Anaerobic

Corynebacterium

Clostridium spp

Bacillus spp

Slide 3

Species of Corynebacteria

Corynebacterium diphtheriae

  • Other Significant Corynebacterium species

    • C. xerosis

    • C. pseudodiphtheriticum

    • C. pseudotuberculosis

    • C. jekeium, (skin)

    • C. ulcerans

Normal flora of RT, urethra, vagina, Skin

Slide 4

Corynebacterium spp

  • Gram positive bacilli, with characteristic morphology (club shaped and beaded)

  • Non motile

  • Non spore forming

  • Non capsulated

  • Non--hemolytic on sheep blood agar

  • Facultative anaerobic

  • C. diphtheriae is fastidious while diphtheriods are non-fastidious

  • Catalase positive

  • Oxidase negative

Slide 5

  • ØLipid-rich cell wall contains meso-diaminopimelic acid, arabino-galactan polymers, and short-chain mycolic acids

  • ØLysogenicbacteriophage encodes for potent exotoxin in virulent strains

Slide 8

Corynebacterium: Natural Habitats

  • Many species normal commensals of the human skin (including

  • C. amycolatum,

  • C. jeikeium,

  • C. urealyticum)

  • C. jeikeium and C. amycolatum also present in the inanimate hospital environment

Slide 9

Corynebacterium: Modes of Infection

  • Corynebacteriumjeikeium, C. amycolatum, and C. urealyticumas skin flora can be introduced systemically from infected catheter wounds

  • Corynebacteriumurealyticumas an anterior urethral commensal can invade the urinary tract in debilitated patients

Slide 10

Corynebacterium: Types of Infectious Disease

  • C. jeikeium, C. amycolatum, and C. urealyticum produce wound infection, bacteremia, and endocarditis in hospital patients.

  • C. urealyticum is a urease producer that causes to deposition of ammonium magnesium phosphate crystals and damages bladder mucosa with ulceration and infection.

Slide 11

Corynebacterium: Types of Infectious Disease

  • Toxigenic strains of C. diphtheriaelyso- genized by tox+-prophage most often cause diphtheria.

  • Occasional strains of C. ulcerans and C. pseudotuberculosis also produce toxin, but only C. ulcerans is associated with diphtheria-like illness.

  • Tox–strains of C. diphtheriae cause pharyngitis and endocarditis.

Slide 12

Cased by C. diphtheriae

Mycocarditis, neuroitis,

Acute, Toxin mediated

Recovery or complication & death (if more toxin absorbed)

Childhood disease affect upper respiratory tract

Diphtheria

Respiratory obstruction due to extensive membrane formation

Transmitted by droplet infection

2-6 days I.P.

Sore throat, Pharyngitis

2-3 days, Bluish white adherent pseudo membrane

Slide 13

Clinical Forms of Diphtheria

  • Respiratory

    • Acquired by droplet spray or hand to mouth contact

    • Non-immunized individuals are susceptible

  • Non-respiratory

    • Systemic

    • Skin and cutaneous forms

Slide 14

C. diphtheriae: Causative Agent of Diphtheria

  • Respiratory disease–diphtheria

    • Incubation period–2 to 5 days

    • Symptoms: sore throat, fever, malaise

    • Toxin is produced locally, usually in the pharynx or tonsils

    • Toxin causes tissue necrosis, can be absorbed to produce systemic effects

    • Forms a tough grey to white pseudomembrane which may cause suffocation

Slide 15

  • . diphtheriae pseudomembrane

    • WBC + organism

  • This may obstruct the  airway  and result  in death caused by a lack of air or oxygen

Slide 16

Corynebacterium

  • The more dangerous  effects  occur when the toxin becomes systemic and  attacks the  heart(heart failure),  peripheral nerves (paralysis), and the adrenal glands (hypofunction).

  • Cutaneous  diphtheria­  More  common  in tropical and subtropical areas.

    • Necrotic lesions with occasional formation of a local pseudomembrane occur.

  • Antibiotic susceptibility and treatment

    • Antiserum ­ once the toxin has bound, however, the antiserum  against it is ineffective.

    • Penicillin­ to eliminate the organism.

  • Slide 17

    Virulence Factors in Corynebacterium Species

    Phospholipase D: increse vascular permeability and promote spread of organism

    Slide 18

    Corynebacterium: Resistance to -Lactam Drugs

    Corynebacteriumamycolatum,

    C. jeikeium, and C. urealyticum are

    characteristically resistant to

    penicillin and other -lactam

    drugs, and uniformly vancomycin

    susceptible

    Slide 19

    • Toxin consists of two fragments

      • A: Active fragment

        • Inhibits protein synthesis

        • Leads to cell/tissue death

      • B: Binding

        • Binds to specific cell membrane receptors

        • Mediates entry of fragment A into cytoplasm of host cell

    • One molecule of toxin can inhibit  90%  of the  protein synthesis in a cell.

    • Systemic effects  include heart failure, paralysis and adrenal hypofunction

  • C. ulcerans and C. pseudotuberculosis sometimes make a diphtheria-like  toxin.

  • Slide 20

    C. diphtheria toxin

    • Toxin enters through receptor mediated endocytosis

    • Acidification of endocytic vesicle allows A to dissociate from B

    • A enters cyctoplasm and inhibit protein synthesis by rection with EF

    Slide 21

    Specific treatment must be never delayed for laboratory results

    To confirm the clinical manifestation

    Slide 22

    Laboratory diagnosis of case

    • Specimen: A throat swap

    • Culture: The swap is inoculated on Loeffler's serum medium and/or on blood tellurite agar aerobically at 37C for 24.

      • On Loeffler's serum medium:

      • Corynebacteria grow much more readily than other respiratory pathogens

      • Deep blue or red metachromatic granules (accumulated inorganic polyphosphates) by methylene blue stain

    Loefflers serum

    Slide 23

    • The colonies of C. diphtheriae are small, granular, grey, smooth, and creamy with irregular edges

    Slide 24

    Cultural characteristics

    • On blood tellurite agar

      • It is selective medium for isolation of C. diphtheriae (Potassium tellurite)

    Slide 26

    • 3 biotypes of C. diphtheriaeare characterized on BTA

    • i.e. Gravis, mitis and intermedius biotypes

    • The most severe disease is associated with the gravis biotype

    • Colony of gravis biotype is large, non-hemolytic & grey.

    • Colonies of mitis biotype are small, hemolytic and black

    • Colonies of intemedius biotype are intermediate in size, non-hemolytic with black center & grey margin.

    Slide 27

    • Morphology

      • Gram +ve, nonspore forming nonmotile bacilli

      • Club-shaped (Coryne= club) arranged at acute angles or parallel to each other (Chinese letters appearance)

      • Beaded (metachromatic granules)

      • Gram stain:

        • C. diphteriae are gram positive bacilli arranged in Chinese letters form often club shaped

    Slide 28

    Biochemical Reaction

    • All Corynebacterium species are catalase positive (Also, Staphylococcus and Bacillus species are catalase positive)

    Slide 29

    2- Carbohydrate Fermentation Test:

    Principle:

    • Each species of corynebacteria has its specific carbohydrate fermentation pattern

    • C.diphtheriae can be differentiated from other Corynebacterium species by fermentation of glucose and maltose but not sucrose with production of acid only

    Slide 30

    Detection of toxin: Elek’s Test

    • Principle:

      • It is toxin/antitoxin reaction

      • Toxin production by C.diphtheriae can be demonstrated by a precipitation between exotoxin and diphtheria antitoxin

    • Procedure:

    • A strip of filter paper impregnated with diphtheria antitoxin is placed on the surface of serum agar

    • The organism is streaked at right angels to the filter paper

    • Incubate the plate at 37C for 24 hrs

    Slide 31

    Filter paper saturated with diphtheria antitoxin

    Lines of precipitations

    • Resuls:

    • After 48 hrs incubation, the antitoxin diffusing from filter paper strip and the toxigenic strains produce exotoxin, which diffuses and resulted in lines four precipitation lines radiating from intersection of the strip and the growth of organism

    Inoculated M.O.

    Positive Elek’s Test

    Slide 32

    Treatment

    • Infected patients treated with anti-toxin and antibiotics

      • Anti-toxin produced in horses

      • Antibiotics have no effect on circulating toxin, but prevent spread of the toxin

        • Penicillin drug of choice with erythromycin


    Copyright © 2014 SlideServe. All rights reserved | Powered By DigitalOfficePro