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Expression level PowerPoint PPT Presentation


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200. 5000. 1500. 5000. ARR4. ARR5. ARR3. 4000. 1200. 4000. 150. 900. 3000. 3000. Expression level. 100. 600. 2000. 2000. 50. 300. 1000. 1000. 0. 0. 0. 0. ARR7. ARR8. ARR6. Expression level. - + - + - + - + BA. - + - + - + - + BA.

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Expression level

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Expression level

200

5000

1500

5000

ARR4

ARR5

ARR3

4000

1200

4000

150

900

3000

3000

Expression level

100

600

2000

2000

50

300

1000

1000

0

0

0

0

ARR7

ARR8

ARR6

Expression level

- + - + - + - + BA

- + - + - + - + BA

- + - + - + - + BA

ARR9

ARR15

ARR16

30 120 min

30 120 min

30 120 min

400

20000

Expression level

250

300

15000

200

200

10000

150

100

100

5000

50

0

0

0

600

1000

AHK1

AHK4

- + - + - + - + BA

- + - + - + - + BA

- + - + - + - + BA

1200

500

800

400

900

Expression level

30 120 min

30 120 min

30 120 min

600

300

600

400

200

300

200

100

0

0

0

- + - + - + - + BA

- + - + - + - + BA

- + - + - + - + BA

30 120 min

30 120 min

30 120 min

400

- + - + - + - + BA

- + - + - + - + BA

300

30 120 min

30 120 min

200

100

0

Col-0

35S:ARR7

Supplementary Figure 1 Analysis of A-type ARRs, AHK1, and AHK4 gene expression in response to cytokinins in wild type and 35S:ARR7. Seven-day-old light-grown seedlings of wild-type and 35S:ARR7 were either mock-treated or treated with 5 mM BA for 30 min or 2 h, total RNA was isolated, and followed by the GeneChip experiments. Cytokinin-regulated A-type ARR and AHK genes that exhibit, on average, >2-fold change are presented. The expression levels (y axis) represent differential hybridizations between perfectly matched and mismatched oligodeoxyribonucleotides and average values of two independent biological experiments. Shaded and thick columns indicate wild-type and 35S:ARR7 transgenic plants, respectively


Expression level

EXPA1

EXPA3

Expression level

600

3000

400

2000

EXPA4

200

1000

Expression level

EXPA5

0

0

- + - + - + - + BA

- + - + - + - + BA

- + - + - + - + BA

30 120 min

30 120 min

30 120 min

1600

600

800

1200

600

400

1200

Expression level

800

400

200

800

EXPA10

400

EXPA6

200

400

0

0

0

- + - + - + - + BA

- + - + - + - + BA

- + - + - + - + BA

0

30 120 min

30 120 min

30 120 min

4000

3000

1600

Expression level

2000

1200

EXPA15

800

1000

0

400

0

- + - + - + - + BA

- + - + - + - + BA

30 120 min

30 120 min

EXPB3

Supplementary Figure 2 Analysis of expansin gene expression in response to cytokinins in wild-type and 35S:ARR7. Experiments were done and analyzed as described in the legend of Supplementary Figure 1


Expression level

LBD4

400

LBD3

300

Expression level

200

100

0

- + - + - + - + BA

- + - + - + - + BA

30 120 min

30 120 min

400

300

200

100

0

Supplementary Figure 3 Analysis of LBD gene expression in response to cytokinins in wild-type and 35S:ARR7. Experiments were done and analyzed as described in the legend of Supplementary Figure 1


Expression level

160

120

120

80

80

40

40

0

0

- + - + - + - + BA

CKX2

CKX3

30 120 min

Expression level

600

1200

800

400

- + - + - + - + BA

200

400

30 120 min

CKX5

CKX4

0

0

Expression level

500

400

200

- + - + - + - + BA

- + - + - + - + BA

CKXa

30 120 min

30 120 min

Expression level

0

- + - + - + - + BA

30 120 min

Supplementary Figure 4 Analysis of cytokinin oxidase gene expression in response to cytokinins in wild-type and 35S:ARR7. Experiments were done and analyzed as described in the legend of Supplementary Figure 1


Expression level

At2g30540

At3g62930

400

300

Expression level

200

100

0

- + - + - + - + BA

- + - + - + - + BA

30 120 min

30 120 min

At1g03850

At4g15680

Expression level

600

6000

4000

400

2000

200

0

- + - + - + - + BA

- + - + - + - + BA

0

30 120 min

30 120 min

400

300

200

100

0

Supplementary Figure 5 Analysis of glutaredoxin gene expression in response to cytokinins in wild-type and 35S:ARR7. Experiments were done and analyzed as described in the legend of Supplementary Figure 1


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