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Exp.7. The measurement of the blood sugar and effects of adrenalin on blood sugar concentration 血糖测定及肾上腺素对血糖浓度的影响. AIM:. 1 、 Study the principle and the methods of the measurement of blood sugar 了解血糖测定的原理和方法;. 2 、 Master the theory of influence of adrenalin on blood sugar concentration

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The measurement of the blood sugar and effects of adrenalin on blood sugar concentration

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Exp.7

The measurement of the blood sugar and effects of adrenalin on blood sugar concentration

血糖测定及肾上腺素对血糖浓度的影响


AIM:

1、 Study the principle and the methods ofthe measurement of blood sugar

了解血糖测定的原理和方法;

2、 Master the theory ofinfluence of adrenalin on blood sugar concentration

掌握肾上腺素对血糖浓度的影响机制


Principle

1. Blood sugar

ATP

absorption

glycogen synthesis

blood sugar

fat or AA

glycogen breakdown

other sugar

gluconeogenesis

glucosuria


Hormone Receptor

+

+

+

+

+

AC

AC

+

cAMP

ATP

2、Regulation of blood sugar

Pi

Phosphorylase b

Phosphorylase a 激活

b Kinase -p

Pi

PKA

PKA

Glycogen synthase

2C

C2R2

Pi

-p 失活


Cu

COONa

CHOH

CHOH

COOK

COONa

CHO

CHO

COOK

3、reaction

(1)、

OH-

Cu2++

+ 2H2O


CHO

(CHOH)4

CH2OH

COOH

(CHOH)4

CH2OH

+ 2H2O +

+ Cu2O

red

3Cu2O + H3PO4•12MoO3H3PO4•6MoO3•3Mo2O3 + 6CuO

Cu

blue

COONa

CHO

CHO

COOK

(2)、

420nm


Operation

?

1、preparation of the blood filtrater without protein

①blooding slowly;

②H2SO4、Na3WO4,dropping and mixing

③Keep the filter paper from wet !!


2、 The measurement of blood sugar

Reagent (ml)

Standard glucose solution

filtrater

H2O

Cu2+ (OH-) solution

磷钼酸试剂

Add H2O to

S

2.00

----

----

2.00

2.00

25.0

U1 U2

---- ----

2.00 2.00

---- ----

2.00 2.00

2.00 2.00

25.0 25.0

O

----

----

2.00

2.00

2.00

25.0

Mix up , 100℃ for 8min , cold water for 3min ,

no shaking

Mix up , remain for 2min (release CO2)


Attentions

1、mix up adequately

2、the length of wave

λ=420nm


ODU

mg% =100×

ODS

Results


discussion

questions 1-4 (P28)


Hormone Receptor

+

+

+

+

+

AC

AC

+

cAMP

ATP

2、Regulation of blood sugar

Pi

Phosphorylase b

Phosphorylase a

Kinase b-p

Pi

PKA

PKA

Glycogen synthase

2C

C2R2

Pi

--

-p


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