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Handling Liquids

Handling Liquids. Robyt and White2.1-2.3; pp. 21-27 Also known as wet chemistry and bench chemistry. Water. Carboys. Hold large quantities 2-20 L Bulk solutions: TAE Buffer, SSC Buffer Remember to crack the lid!. Erlenmeyer Flasks. 125 ml -4 liters Melting agar media, agarose

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Handling Liquids

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  1. Handling Liquids Robyt and White2.1-2.3; pp. 21-27 Also known as wet chemistry and bench chemistry

  2. Water

  3. Carboys • Hold large quantities 2-20 L • Bulk solutions: TAE Buffer, SSC Buffer • Remember to crack the lid!

  4. Erlenmeyer Flasks • 125 ml -4 liters • Melting agar media, agarose • Can be heated and autoclaved • Not intended for storage

  5. Beakers • 100 ml – 2 liters • Start solutions, media without agar • Can be heated • Not intended for storage

  6. Screw-cap Bottles • Volumes 100 ml to 1000 ml • Cheap, autoclaveable, reusable • Molecular Biology reagents, Liquid Media • Do not put on hot plate! • Storage

  7. Conical Tubes • Plastic, sterile, disposable centrifuge tubes • 15 ml or 50 ml size • Grow bacteria – 5ml media in 50 ml tube • Filter-sterilized solutions and media • Great for storage (including freezing)

  8. Microcentrifuge Tubes • Plastic, sterile, disposable centrifuge tubes • 2, 1.5, 0.5, and 0.2 (microamp) formats • Most molecular techniques, small reaction volumes • Special racks and storage

  9. Graduated Cylinders vs Volumetric Flasks

  10. Pipetters • Air-displacement piston pipettes • Disposable, sterile, plastic tips • Volumes 0.5 ml – 1000 ml

  11. Downstream

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