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Supplementary Figure 2. Expression of TLR2, TLR4, TLR9, and MyD88 in mild neurogenic muscular atrophy (case #22). a-d: TLR2 (a), TLR4 (b), TLR9 (c), and MyD88 (d) are not expressed. All are double-stained with mouse anti-CD68 FITC; note the single CD68-positive macrophages. Double immunofluorescence with: rabbit anti-human TLR2 with goat anti-rabbit DyLight 549 red (a), rabbit anti-human TLR4 with goat anti-rabbit DyLight 549 red (b), rabbit anti-human TLR9 with goat anti-rabbit DyLight 549 red (c) and goat anti-human MyD88 with rabbit anti-goat BSP and Extravidin Cy3 (d), respectively; original magnification x400.