Molecular tools for aiding and breeding of aquaculture species
Download
1 / 20

Molecular Tools for Aiding and Breeding of Aquaculture Species - PowerPoint PPT Presentation


  • 80 Views
  • Uploaded on

Molecular Tools for Aiding and Breeding of Aquaculture Species. Masters in Aquaculture and Fisheries Genetic and Selection 2014 Tamara Moedas nº 47853; Catarina Ferreira nº 35671. Molecular Tools. Allozyme markers; Mitochondrial DNA markers (mtDNA);

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Molecular Tools for Aiding and Breeding of Aquaculture Species' - elata


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
Molecular tools for aiding and breeding of aquaculture species

Molecular Tools for Aiding and Breeding of Aquaculture Species

Masters in Aquaculture and Fisheries

Genetic and Selection

2014

Tamara Moedas nº 47853; Catarina Ferreira nº 35671


Molecular tools
Molecular Tools Species

  • Allozyme markers;

  • Mitochondrial DNA markers (mtDNA);

  • Restriction fragment length polymorphism (RFLP);

  • Random amplified polymorphic DNA (RAPD);

  • Amplified fragment length polymorphism (AFLP);

  • Microsatellite markers;

  • Single nucleotide polymorphism (SNP);

  • Expressed sequence tags (ESTs).



  • Advantages Species:

  • Lowcost;

  • the rapidtechnique;

  • Ease to use;

  • Usuallyused in fisherygenetics.

  • Disadvantages:

  • Heterozygotedeficienciesdue to nullalleles;

  • Limitednumber of availableallozymelociprecludestheir use in large-scalegenome mapping;

  • Changesin DNA sequencereducethelevelofdetectablevariation

  • Are not selectively neutral.


Mitochondrial dna markers
Mitochondrial Species DNA Markers


  • Advantages Species:

  • Highlevelsofpolymorphism;

  • It evolves much faster than coding regions of DNA;

  • Multiple copies in each cell.

  • Disadvantages:

  • Backmutation;

  • Parallelsubstitution;

  • Rate heterogeneityormutational hot spots.



  • Disadvantages:

  • Low level of polymorphism;

  • Low power in revealing genetic variation;

  • Difficultand time-consumingto developmarkers in specieslackingmolecular information.


Random amplified polymorphic dna markers rapd
Random SpeciesAmplifiedPolymorphic DNA Markers (RAPD)


  • Advantages Species:

  • Highlevelsofpolymorphisms;

  • Allowsthesimultaneousanalysisofmanyloci;

  • Markers are inherited in dominantfashion.

  • Disadvantage:

  • Special equipment.


Amplified fragment length polymorphism aflp
Amplified Fragment SpeciesLength Polymorphism (AFLP)


  • Advantages: Species

  • Analysismanylocisimultaneously;

  • Revealedpolymorphisms;

  • Inheritedas dominantmarkers;

  • Highreproducibility.

  • Disadvantage:

  • Special equipment.



  • Advantages: Species

  • HightAbundantin allspecies;

  • Distributedin thegenomeonallchromosomes;

  • Lociare small;

  • Polymorphic;

  • Codominant markers.

  • Disadvantage:

  • Hightexpensive.


Single nucleotide polymorphism snp
Single SpeciesNucleotidePolymorphism (SNP)


  • Advantages: Species

  • Mostabundantpolymorphism in organism;

  • Revealhiddenpolymorphism;

  • Co-dominant markers.

  • Disadvantages:

  • Requiresspecializedequipment;

  • Large-scaleanalysis, dependson the availability of expensive, cutting-edgeequipment.


Expressed sequence tags ests
Expressed Speciessequencetags(ESTs)


  • Advantages: Species

  • Identifygenes andanalyzetheirexpressionbyexpressionprofiling;

  • Rapidandat genes expressedin specifictissuetypes;

  • Developmentof cDNAmicroarrays;

  • Great value in genome mapping.

  • Disadvantage:

  • Identification of polymorphic ESTs is necessary for genome mapping.


Conclusions
Conclusions Species


Biblography
Biblography Species

  • Liu, Z. J., Cordes, J. F., 2004. “DNA marker technologies and their applications in aquaculture genetics”. Aquaculture 238, pp. 1-37;

  • Magoulas, A.. “Application of molecular markers to aquaculture and broodstock management with special emphasis on microsatellite DNA”. CIHEM-Options Mediterraneennes, pp. 153-168;

  • Chauhan,T.,Rajiv, K.,2010. “Molecular markers and their applications in fisheriesandaquaculture”.UniversityofDelhi, Delhi, India.


ad