Download
1 / 1

*** - PowerPoint PPT Presentation


  • 109 Views
  • Uploaded on

A. B. ***. **. ***. ***. ***. ***.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' ***' - elani


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript

A

B

***

**

***

***

***

***

For osteoclast-T cell co-culture, bone marrow cells (BMCs) were isolated from 4- to 6-week-old mice by flushing the bone marrow from the excised femora with osteoclast cell culture medium containing 10% FCS. After overnight culture in this medium, non-adherent bone marrow cells were added to purified CD4+CD28null T cells in 24-well plates in osteoclast culture medium containing M-CSF and RANKL and culture was continued till day 6. At the end of experiment conditioned media was collected to perform TNF-α ELISA (A). The transcript level of tartarate resistant acid phosphatase (TRAP) in co-culture was determined by q-PCR (B). ***P < 0.001


ad