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Борисова П. Научны e руководители: Волкова П.А. Ильинский В.В. PowerPoint PPT Presentation


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Поиск диагностических морфологических признаков и генетических маркеров у ели европейской ( Picea abies ) и ели сибирской ( P . obovata ) и определение видовой принадлежности елей Северной Карелии. Борисова П. Научны e руководители: Волкова П.А. Ильинский В.В. Введение . P. obovata.

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Борисова П. Научны e руководители: Волкова П.А. Ильинский В.В.

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E

(Picea abies) (P. obovata)

.

e :

..

..


E

P. obovata

P. abies


E

P. abies

P. obovata


E

P. abies P. obovata .


E

141 (149 ), 10

(4)

(1)

(2)

(3)


E


E

P. obovata

P. abies

:

4-6 (8)

10-16


E

:

DSL/HS*100

:

(LS-DS)/HS*100

:

P. abies

P. obovata

5

5


E


E

  • , ;

  • :


E


E

( )


E

.

P. obovata

P. abies


E

.

P. obovata

P. abies


E

.

P. obovata

P. abies


E


E


E

-

P. abies

P. obovata

, =>

(Single nucleotide polymorphism, SNP)


E

( )

()


E

( )

4

  • (220 467),

  • ()

  • (TRN)


E

58,

10

60, 10

62,10

61, 10

64, 5

60, 5

65, 5

67, 5


Snp 427 gb genbank

SNP 427 .

GB GenBank


Snp 220 gb genbank

SNP (220)GB GenBank


E

  • , P. abies P.obovata. SNP .

  • P. abies, P.obovata , , .

  • , , P. fennica, P. obovata.


E

- (1543).

; . , . , . , . , ., . .

.. ,

.

. . . .


E


E

  • 20

  • tpsDig

  • , , tpsRelw

  • tpsSuper

  • tpsUtil


E


E

  • .

  • R


E

(CP)


E

(CN)


Dc lc

(DC/LC)


E


E

  • . 10-20 500 (0,2 Tris-HCl pH 8,0, 0,05 EDTA, 2 NaCl, 2% ), . - (Chomczynski, Sacchi, 1987):

  • 400 pH=7,2, .

  • 400 (24:1), .

  • 10 13000 / (16000 g).

  • 1,5 800 100% , .

  • 5 13000 /.

  • .

  • 400 70% , .

  • 5 13000 /

  • ( 10 ).

  • 50 TE (Tris EDTA) 65 .

  • 20 .


E

25

  • 17,5

  • 2,5 10- -

  • 0,5 dNTP's (25 )

  • 0,15

  • 1

  • 0,5 HS Taq- ( )


E


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