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Optimization of Aquaponics System in Hawaii: Preliminary data. Marissa Lee University of Hawaii, Manoa: College of Tropical Agriculture and Human Resources Department: Molecular Bioscience and Bio-Engineering November 21, 2009 Bilger Hall 150. Presentation Agenda. Project Rationale

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optimization of aquaponics system in hawaii preliminary data
Optimization of Aquaponics System in Hawaii: Preliminary data

Marissa Lee

University of Hawaii, Manoa: College of Tropical Agriculture and Human Resources

Department: Molecular Bioscience and Bio-Engineering

November 21, 2009

Bilger Hall 150

presentation agenda
Presentation Agenda
  • Project Rationale
  • Experiment 1: Effects of Lighting
    • Shade
    • No Shade
  • Experiment 2: Effects of Different Feeds
    • Protein Content
    • Fat Content
project rationale
Project Rationale
  • Partial fulfillment of MBBE graduation requirement
    • Senior thesis
  • Partial fulfillment of CTAHR vision and mission
    • Generate and transfer science based information for stake holders to make informed decisions.
slide4
Experiment 1: Test the effects of light
    • How are nutrients in fish effluent affected?
    • How are the fish’s growth affected?
    • What is the crop output per treatment?
the experiment was held at uh freshwater aquaculture facility at windward community college
The experiment was held at UH Freshwater Aquaculture Facility at Windward Community College
experiment 1
Experiment 1
  • Fish:Oreochromis sp.
  • Plant:Lactuva sativa- Red sail Lettuce
experimental design tank setup
Each tank stocked with 323 Tilapia.

Average body weight = 1.64g

Stocking density = 0.28 kg/m3

Salmon Starter Feed

52% crude protein

16% crude fat

Biofilm

Experimental Design: Tank Setup
lettuce trays
Lettuce Trays

Flow rate =

1.35 seconds to fill 1 Liter

temporal changes in dissolved oxygen in covered and uncovered tanks
Temporal Changes in Dissolved Oxygen in Covered and Uncovered Tanks

p < 0.01

=Difference is statistically significant

temporal changes in ph in covered and uncovered tanks
Temporal Changes in pH in Covered and Uncovered Tanks

p < 0.01

=Difference is statistically significant

slide14
Turbidity tube

 measuring the amount of particles.

p < 0.01

=Difference is statistically significant

slide16

P < 0.0001

=Difference is statistically significant

slide20
Experiment 2: Effects of different feeds
    • How are nutrients in fish effluent affected?
    • Is fish growth affected?
    • Is crop out put affected?
experiment 2
Experiment 2

Plant:Lactuva sativa- Red sail Lettuce

Fish:Oreochromis sp.-Tilapia

Plant:Brassica juncea- Mustard Cabbage

Plant: Lactuva sativa-Manoa Lettuce

experiment 2 two treatments
Treatment 1- Rangen 350

Treatment 2- Silver Cup

Experiment 2:Two treatments

Silver Cup

Rangen 350

different treatments
Rangen:

Crude Protein………35.0%

Crude Fat…………….5.0%

Crude Fiber…………..5.0%

Ash…………………..10.0%

Phosphorus…………..1.0%

Silver Cup:

Crude Protein…….45.0%

Crude Fat………...16.0%

Crude Fiber.……….3.0%

Ash………………..12.0%

Phosphorus………..1.2%

Different Treatments

$ 0.77/ lb

$ 0.63/ lb

experiment design tank setup
Each tank was stocked with 200 Tilapia

Tank average = 26.4 g

Stocking density = 2.78 kg/ m3

Bioflim

Experiment Design: Tank Setup
slide27

Trace Minerals in Water

  • Rangen
    • N = 14.9 ppm
    • P = 1.0
    • K = 10.6
    • Ca = 6.0
    • Mg = 5.1
    • Na = 10.9
    • Fe = 0.1**
    • Mn = 0.1**
    • Zn = 0.04
    • Cu = 0.01
    • B = 0.03
  • Silver Cup
    • N = 17.8 ppm
    • P = 1.9
    • K = 7.7
    • Ca = 9.8
    • Mg = 5.0
    • Na = 6.3
    • Fe = 0.1**
    • Mn = 0.1**
    • Zn = 0.1
    • Cu = 0.0
    • B = 0.0

Agricultural Diagnostic Service, ** = supplmented

slide28

Analysis of Covariance

p > 0.05

=not statistically different

T-Test for last sampling day

p > 0.05

=not statistically different

slide29

p < 0.05

Silver Cup

Rangen

slide30

p< 0.01

Silver Cup

Rangen

slide31

p>0.05

Silver Cup

Rangen

acknowledgements
Acknowledgements
  • Clyde Tamaru
  • Kathy McGovern-Hopkins
  • Harry Ako
  • Mom and Dad
  • My friends
ad