1 / 1

Targeted disruption at exon 9 of the PARP-2 gene by homologous recombination in mouse

Targeted disruption at exon 9 of the PARP-2 gene by homologous recombination in mouse. HpaI. EcoRV. BglII. Targeting vector. PGK-hygro. HpaI. BclI. B. B. Bglll. BclI. PARP-2 locus. E9. 4.5. HpaI. EcoRV. BgIII. BclI. Targeted allele. +/+. -/-. +/+. -/-. +/+. -/-.

edmund
Download Presentation

Targeted disruption at exon 9 of the PARP-2 gene by homologous recombination in mouse

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Targeted disruption at exon 9 of the PARP-2 gene by homologous recombination in mouse HpaI EcoRV BglII Targeting vector PGK-hygro HpaI BclI B B Bglll BclI PARP-2 locus E9 4.5 HpaI EcoRV BgIII BclI Targeted allele +/+ -/- +/+ -/- +/+ -/- +/+ -/- PGK-hygro PARP-1 3.7 PARP-2 +/+ +/- -/- -/- -/- +/- +/- +/+ 4.5 kb actin 3.7 PARP-2 RNAse P EtBr ES stem cells Tail DNA Western blot Northern blot Southern blot Testis cell extracts Ménissier - de Murcia et al. 2003, EMBO J. 22, 2255

More Related