cutA - mutant#2 Complementation 실험 준비 중 .

1. orf2- mutant 1. Mutation이 일어난 copy확인 위해 Ch’mal DNA 추출. 2. 다른 copy에서 mutagenesis를 위해 electoporation 수행. orf6- mutant Complementation 실험 준비 중. CO-DH 유전자의 mRNA가 합성되지 않음. (Northern blot으로 확인) Regulator일 가능성 확인… orf6- mutant에서 promoter assay orf6- mutant + promoter region of CO-DH genes Wild type + promoter region of CO-DH genes Plasmid 확인 후, 성장 시기별로 promoter asssay 수행 예정 cutA- mutant#2 Complementation 실험 준비 중.

2. Comparison of amino acid sequence of DHAS from Mycobacterium sp. strain JC1 Mycobacterium sp. strain JC1  Arthrobacter sp. FB24 Mycobacterium smegmatis Mycobacterium avium Mycobacterium leprae Mycobacterium tuberculosis H37Rv Mycobacterium bovis Mycobacterium tuberculosis CDC1551 Comparison of nucleotide sequence of DHAS from Mycobacterium sp. strain JC1 Mycobacterium sp. strain JC1  Arthrobacter sp. FB24 Mycobacterium smegmatis Mycobacterium avium Mycobacterium leprae Mycobacterium tuberculosis H37Rv Mycobacterium bovis Mycobacterium tuberculosis CDC1551

3. Comparison of amino acid sequence of DHAS from Mycobacterium sp. strain JC1 Identity Positivity 56% 55% 56% 55% 53% 57% 79% 66% 65% 66% 66% 63% 68% 86% Mycobacterium tuberculosis H37Rv Mycobacterium tuberculosis CDC1551 Mycobacterium bovis Mycobacterium leprae Mycobacterium smegmatis Mycobacterium avium Arthrobacter sp. FB24 Comparison of nucleotide sequence of DHAS from Mycobacterium sp. strain JC1 Identity 64% 64% 64% 61% 60% 65% 78% Mycobacterium tuberculosis H37Rv Mycobacterium tuberculosis CDC1551 Mycobacterium bovis Mycobacterium leprae Mycobacterium smegmatis Mycobacterium avium Arthrobacter sp. FB24

4. DHAS

5. 624 bp 약 570 bp (JC1)