Fig. S2
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Fig. S2. P1. P2. P3. 23.0. slope: -3.076 Intercept: 21.336 R2: 0.999. slope: -3.235 Intercept: 18.389 R2: 0.992. slope: -3.403 Intercept: 21.734 R2: 0.999. 24.5. 23.5. 18.0. 18.5. 17.5. Ct. 13.5. 12.5. 12.0. 2. -1. 0. 1. 3. -1. 0. 1. 2. 3. 2. -1. 0. 1. 3. L1.

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Fig. S2

P1

P2

P3

23.0

slope: -3.076

Intercept: 21.336

R2: 0.999

slope: -3.235

Intercept: 18.389

R2: 0.992

slope: -3.403

Intercept: 21.734

R2: 0.999

24.5

23.5

18.0

18.5

17.5

Ct

13.5

12.5

12.0

2

-1

0

1

3

-1

0

1

2

3

2

-1

0

1

3

L1

R1

30.4

slope: -3.243

Intercept: 27.150

R2: 0.998

slope: -3.382

Intercept: 25.040

R2: 0.999

30.0

24.5

24.0

18.0

18.5

-1

1

2

0

3

2

-1

0

1

3

Total DNA (log ng)

Fig. S2 Correlationbetween the amount of template DNA and threshold cycle number (Ct) for each primer/probe set. Vertical and horizontal axes show strength of fluorescence and the amount of DNA (log ng), respectively. Slope, intercept, and coefficient of correlation(R2)are provided in the upper right of each panel. Genomic DNA of MAFF 103036 (race 1) was used for P1, L1, and R1. JCM 12575 (race 2) and H-1-4 (race 3) were used for P2 and P3, respectively.


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