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100000. 10000. 1000. Number of non-redundant consensus. 100. 10. 1. 100-500. 501-1000. 1001-2000. 2000-5000. >5000. The length of non-redundant consensus sequences (bp). Fig. S1 Distribution of the non-redundant consensus sequences. A:Amino acid transport and metabolism

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  1. 100000 10000 1000 Number of non-redundant consensus 100 10 1 100-500 501-1000 1001-2000 2000-5000 >5000 The length of non-redundant consensus sequences (bp) Fig. S1 Distribution of the non-redundant consensus sequences.

  2. A:Amino acid transport and metabolism B:Carbohydrate transport and metabolism C:Cell cycle control, cell division, chromosome partitioning D:Cell wall/membrane/envelope biogenesis E:Cell motility F:Chromatin structure and dynamics G:Coenzyme transport and metabolism H:Cytoskeleton I:Defense mechanisms J:Energy production and conversion K:Extracellular structures L:Function unknown M:General function prediction only N:Intracellular trafficking, secretion, and vesicular transport O:Inorganic ion transport and metabolism P:Lipid transport and metabolism Q:Nucleotide transport and metabolism R:Nuclear structure S:Posttranslational modification, protein turnover, chaperones T:Replication, recombination and repair U:RNA processing and modification V:Secondary metabolites biosynthesis, transport and catabolism W:Signal transduction mechanisms X:Transcription Y:Translation, ribosomal structure and biogenesis 4000 3000 2000 Frequency 1000 0 A B C D E F G H I J K L M N O P Q R S T U V W X Y Function Class Fig. S2 Histogram presentation of the clusters of eukaryotic orthologous groups (KOGs). Of the 93,488 identified unigenes, 29,828 sequences have a KOG classification within the 25 categories.

  3. 350 300 250 200 Number of genes 150 100 50 0 B3 Dof EIL SBP SRS ERF CPP AP2 HSF TCP LFY C3H GRF ARF VOZ LBD NAC bZIP DBB RAV MYB WOX BES1 GeBP C2H2 STAT MIKC TALE bHLH GATA GRAS NF-X1 E2FDP M-type NF-YB NF-YC ZF-HD G2-like NF-YA WRKY ARR-B HD-ZIP Trihelix CO-like Nin-like S1Fa-like HB-other CAMTA BBR/BPC HB-PHD HRT-like MYB_related Fig. S3 The number of unigenes corresponding to transcription factors in each transcription factor family. A total of 2,023 putative Arabidopsis thaliana transcription factors were searched against the Antarctic moss transcriptome, and the target unigenes (1,309) were classified into their corresponding transcription factor families. The black bars are the number of reads per family.

  4. 300 More reliable points Less reliable points 250 200 150 -Lg(p-value) 100 50 0 -5 0 5 Log2Ratio(Treat/Control) Fig. S4 A volcano plot of the differentially expressed genes. For each gene, the ratio of the expression levels in the treated sample versus that of the control sample was plotted against the -log error rate. The horizontal green line indicates the significance threshold (0.001), and the two vertical green lines indicate the fold-change threshold (log2(Treat/Control)≥1 or log2(Treat/Control)≤ -1).

  5. Cold treatment 0hr 1hr 3hr 6hr 12hr 24hr MFC60113 MFC00614 MFC48132 MFC03221 MFC48409 MFC08686 MFC04509 MFC90936 MFC25111 tubulin Fig. S5 RT-PCR analysis of CRLK1, MEKK1, MKK2, MPK4 and CBF orthologs in Antarctic moss P. nutans after cold stress. MFC60113 is CRLK1 ortholog; MFC00614, MFC48132, MFC03221, MFC48409 and MFC08686 are MEKK1 orthologs; MFC04509 is MKK2 ortholog; MFC90936 is MPK4 ortholog; MFC25111 is CBF ortholog. β-tubulin was used to normalize the template.

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