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Revision – Concept map

Revision – Concept map. Human Genome Project. Procedures Genetic mapping Physical mapping DNA sequencing Techniques Use of microsatellites (STRs) Restriction enzyme mapping Sanger method PCR Gel electrophoresis. Human Genome project. Terminology Restriction mapping Primer

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Revision – Concept map

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  1. Revision – Concept map

  2. Human Genome Project • Procedures • Genetic mapping • Physical mapping • DNA sequencing • Techniques • Use of microsatellites (STRs) • Restriction enzyme mapping • Sanger method • PCR • Gel electrophoresis

  3. Human Genome project • Terminology • Restriction mapping • Primer • Microsatellite / Short Tandem Repeat • Anneal • Genetic marker • Hybridise

  4. Detecting Genetic Disorders • Procedures • Pedigree analysis • Linkage analysis (both genetic and physical) • Screening • Techniques • PCR • Chromosome walking • Sanger method • Gel electrophoresis

  5. Detecting genetic disorders • Terminology • Probe • Genomic library • Chromosome walking • Hybridise

  6. DNA profiling • Procedures • DNA isolation • Restriction enzyme digestion • Separation of VNTR • Fixing pattern on filter • Techniques • Restriction enzyme digestion • Gel electrophoresis • Southern blotting

  7. DNA profiling • Terminology • Minisatellite / Variable Number Tandem Repeat • Probe • Hybridise

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