Toxicity of automotive runoff on daphnia
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Toxicity of Automotive Runoff on Daphnia. Morgan Dutrow 10 th Grade Bellwood-Antis. Why I Chose this Experiment. I chose this experiment because…. Daphnia could be an indicator of what will happen when chemicals runoff of parking lots and into aquatic environments.

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Toxicity of Automotive Runoff on Daphnia.

Morgan Dutrow

10th Grade

Bellwood-Antis


Why I Chose this Experiment

I chose this experiment because…

  • Daphnia could be an indicator of what will happen when chemicals runoff of parking lots and into aquatic environments.

  • Earlier in the year I had learned a little about Daphnia.


Background Information

  • Brake fluid

  • Contains: Diethylene glycol (C4O3H)

Windshield Washer Fluid

  • Contains: Methanol (CHO3H)

  • Antifreeze

  • Contains: Ethylene glycol (C2H6O2)

Lethal Dose 50 in 96 hours


Hypothesis

Windshield washer fluid be the least lethal.


Why I Chose Daphnia…

  • In our experiment we used the variety of daphnia known as Daphnia Magnum.

  • Daphnia being much smaller, would be more susceptible. Because they are a food source, this could then work its way up the ecosystem.

  • Daphnia are an indicator species.


General Procedure

  • 40ml fluid with 4 daphnia

  • 3 trials of each dilution


Making a dilution: Ex. 1/10 dilution

  • Water: 34ml

  • Automotive pollutant: 4ml

  • Food:2ml


Recipes For My Dilutions

DilutionWaterFoodChemical

1/2 18 2 20

1/5 30 2 8

1/10 34 2 4

1/20 36 2 2

1/40 37 2 1

1/80 37 ½ 2 ½


Experimental Design

  • Variable

    • the pollutant

  • Experimental Group

    • Antifreeze, Brake Fluid, and Windshield Washer Fluid

  • Control Group

    • Spring Water


Experimental Design Contd.

  • Control Variables:

    Amount of…

    • Light

    • Food

    • Chemical

    • Water

      Others…

    • Temperature

    • Size of daphnia

    • Clean materials


LD 50

  • Daphnia died too close together


Daphnia Lifetime (in minutes) inBrake Fluid


Averages Daphnia Lifetimes of 3 Trials

Note: All numbers are in minutes.


Conclusion

According to my data, I reject my hypothesis that Windshield Washer Fluid would be the least lethal to daphnia.


Improvements

  • I would…

    • use more specific dilutions.

    • use different animals.

    • use more chemicals.


Expanding my Project

  • I could…

    • test motor oil by using a larger aquatic animal.

    • test to see what affect making the water more acidic or basic would have.


Bibliography

  • http://ijt.sagepub.com/cgi/content/abstract/23/1/55 1/24

  • http://www.aehms.org/jaeh_3_2_sherry.html 1/24

  • http://www.jtbaker.com/msds/englishhtml/e5125.htm 2/8

  • https://fscimage.fishersci.com/msds/89493.htm 2/8

  • http://www.atsdr.cdc.gov/tfacts96.html 2/10


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