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实验三 血清补体总活性测定 —— CH50 试验 PowerPoint PPT Presentation


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实验三 血清补体总活性测定 —— CH50 试验. 补体 :一组存在于人和脊椎动物血清中及组织液中具有酶样活性,不耐热和功能上连续反应的糖蛋白,是抗体发挥溶细胞作用的必要补充条件,在正常情况下,血清中的补体大多以 无活性酶前体 形式存在。. 物理性质:不稳定,易受各种理化因素的影响,加热,紫外线照射,机械震荡,酸碱和酒精等因素均可破坏补体。 灭活: 56℃ 加热 30min 即可灭活. 补体 系统 的生物学功能. ( 1 ) 溶菌和溶细胞作用 :补体系统激活后,在靶细胞表面形成 MAC ,从而导致靶细胞溶解。

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实验三 血清补体总活性测定 —— CH50 试验

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Ch50

CH50


Ch50


Ch50

  • 5630min


Ch50

  • 1MAC

  • 2C3bC4biC3b


Ch50

  • 3C3aC4aC5aC5a


Ch50

  • 4IgFcICICICC3bICC3bCR1CR3


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  • 5C3APC


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  • MBL


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1(classical pathway)C1C4C2C3C5C9


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  • 2:C1C4C2BDPC3b


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  • 3MBL(MBL)CMBLMASPMBLMASPC4C2


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  • 3-54


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  • SRBC5630min60 3min


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  • CH50

  • CH50


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S

S3070505050CH50

50%50%


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  • BB22U


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1.1:20

2. 50%

12%SRBC 0.5ml+ ddH2O 2.0ml

2500rpm 10min,

250%

1.25ml+1.25mlBB


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4.110ODOD

U/ml/50


Ch50

1.

2.2

3.20

4.SRBCSRBC

5.2SBC


Ch50

1.

2.

  • SLERA


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CH50


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