Adventures in HRP. Ellyn Daugherty San Mateo Biotech Career Pathwaywww.SMBiotech.com AEEDaugher@aol.comwww.BiotechEd.com EMC/Paradigm Publishingwww.emcp.com/biotech VWR/Sargent-Welchwww.sargentwelch.com. San Mateo Biotechnology Career Pathway www.SMBiotech.com.
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The HRP Activities are found in Chapter 6
You need 100 mL of a 25 mM NaH2PO4 * H20, pH 7 buffer.
conc. desired X volume desired X molecular weight = amount of solute
in mol/L in L in g/mol in g
Convert 25 mM = 0.025 mM and convert 100 mL = 0.1 L
0.025 mol/L X 0.1 L X 138 g/mol = 0.345 g NaH2PO4 * H20
0.345 g of NaH2PO4 * H20 is measured measured on a balance and put into an appropriate vessel. Then, solvent is added up to the 100 mL graduation.
Adjust pH to 7 and add dH20 to 100 mL
add solvent (dH20) to 75 mL
0.345 g NaH2PO4 * H20
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