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Adventures in HRP. Ellyn Daugherty San Mateo Biotech Career EMC/Paradigm VWR/ San Mateo Biotechnology Career Pathway

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Adventures in hrp

Adventures in HRP

Ellyn Daugherty

San Mateo Biotech Career



Adventures in hrp

San Mateo Biotechnology


Giving motivated teenagers and adults the skills to pursue several career and academic options.

Partnerships with industry and academia

Partnerships with Industry and Academia

Adventures in hrp

Biotechnology is an opportunity to teach science in a way that results in science literacy & research skills & career awareness for virtually every student.

  • Students learn the skills and gain the confidence and self-directedness that are necessary for independent research(science fair projects) and higher-level science courses.

  • Students see meaning and application in their math, biology and chemistry.

  • Students learn the science and business of biotech so that they are better prepared to make decisions about their futures and society.

The hrp activities are found in chapter 6

The HRP Activities are found in Chapter 6

  • Text - Chapter 6 and 14

  • Lab Manual - Labs 6f-6h, 14a

  • Student CD Tutorials for basic skills

  • Course Planner/Instructor’s Guide

Biotech 3 hrp labs

Biotech 3 HRP labs:

  • Peroxidase Assay

  • HRP extraction from HR Root

  • HRP assay using PAGE

  • HRP assay w/ TMB indicator

  • HRP used in ELISAs

How do you make the phosphate buffer

How Do You Make the Phosphate Buffer?

You need 100 mL of a 25 mM NaH2PO4 * H20, pH 7 buffer.

conc. desired X volume desired X molecular weight = amount of solute

in mol/L in L in g/mol in g

Convert 25 mM = 0.025 mM and convert 100 mL = 0.1 L

0.025 mol/L X 0.1 L X 138 g/mol = 0.345 g NaH2PO4 * H20

0.345 g of NaH2PO4 * H20 is measured measured on a balance and put into an appropriate vessel. Then, solvent is added up to the 100 mL graduation.

100 mL

Adjust pH to 7 and add dH20 to 100 mL

add solvent (dH20) to 75 mL

0.345 g NaH2PO4 * H20

Hrp is 43 kd

HRP is 43 kD

50 kD

35 kD

Adventures in hrp

My Professional Goals


  • Biotechnology programs in everyhigh school, CTE center, and community college, tailored to the student population and industry and community needs

  • Aligned and articulated programs in HS and CC so that teenagers and adults have the same platform from which to enter specialized programs

  • To make it as easy as possible for educators to do the other 2

Adventures in hrp

Adventures in hrp

Get help starting or improving a biotech program

Get help starting or improving a biotech program !

Ellyn Daugherty

SM Biotech Career Pathway

JEFF GANDY 800.848.9033

Holly Ahern



Sandy Duffey

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