Laser Induced Fluorescence Transient (LIFT):
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Laser Induced Fluorescence Transient (LIFT): remote measurement of light use efficiency in ecosystems. Roland Pieruschka, Uwe Rascher, Denis Klimov, Zbigniew S. Kolber, Joseph A. Berry. February, 08. 2007. Objectives. Inaccessible outer canopies - need for remote

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Laser Induced Fluorescence Transient (LIFT): remote measurement of light use efficiency

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Laser induced fluorescence transient lift remote measurement of light use efficiency

Laser Induced Fluorescence Transient (LIFT):

remote measurement of light use efficiency

in ecosystems

Roland Pieruschka, Uwe Rascher, Denis Klimov, Zbigniew S. Kolber, Joseph A. Berry

February, 08. 2007


Laser induced fluorescence transient lift remote measurement of light use efficiency

Objectives

  • Inaccessible outer canopies - need for remote

  • measurement of light use efficiency

  • Evaluation of the LIFT approach by PAM

  • fluorometry and gas exchange

  • Measurement of cold/light stress of outer canopies

  • in the field

  • Implementation of the data into canopy models

  • to reduce their uncertainty


Laser induced fluorescence transient lift remote measurement of light use efficiency

LIFT – Fluorometer

  • remote measurement: from a distance up to 50 m

  • excitation signal: 665 nm laser diode

  • fluorescence emission at 690 nm: collected

  • by Cassegrian telescope and detected

  • by avelange photodiode


Laser induced fluorescence transient lift remote measurement of light use efficiency

Fast Repetition Rate Fluormetry

(Kolber et al. 1998; Kolber et al. 2005. Ananyev et al. 2005)


Laser induced fluorescence transient lift remote measurement of light use efficiency

(Govindjee, 1995)

What are we measuring?

single vs. multiple turnover

  • FRR: sequence of single turnover fleshlets:

  • corresponds to O-J phase

  • (photochemical phase)

  • kinetics of QA- accumulation/re-oxidation

  • with minimal effect on PQ pool

  • PAM: multiple photochemical turnover:

  • corresponds to O-J-I-P phase (photochemical and thermal phase)

  • well defined final state with fully reduced PQ pool


Laser induced fluorescence transient lift remote measurement of light use efficiency

Laboratory experiments: bench top FRR vs. PAM

  • Combined measurements of:

  • gas exchange (LI-6400)

  • chlorophyll fluorescence

  • using PAM and a bench top FRR

  • measurements under non-

  • photorespiratory conditions


Laser induced fluorescence transient lift remote measurement of light use efficiency

Laboratory experiments: bench top FRR vs. PAM

FRR

PAM


Laser induced fluorescence transient lift remote measurement of light use efficiency

Laboratory experiments: bench top FRR vs. PAM


Laser induced fluorescence transient lift remote measurement of light use efficiency

Electron Transport Rates

gas exchange vs. fluorescence

PAM

FRR

(Genty et al. 1989)


Laser induced fluorescence transient lift remote measurement of light use efficiency

Electron Transport Rates

from FRR fluorescence and gas exchange


Laser induced fluorescence transient lift remote measurement of light use efficiency

PSII quantum efficiency vs. quantum yield

of CO2 assimilation


Laser induced fluorescence transient lift remote measurement of light use efficiency

Corrected ETR

Estimate of underestimation

of ETR by FRR

4*(A+Rd)/PAR vs. Fv/Fm


Laser induced fluorescence transient lift remote measurement of light use efficiency

Summary I

  • The LIFT/FRR is working with low excitation intensities

  • resulting in lower maximum fluorescence then

  • the PAM approach in particular under high light intensities

  • However, the LIFT approach provides an advantage of remote

  • measurement of Fv Fm-1’ as an index of stress correlated

  • to ETR within undisturbed microenvironment of leaves


Laser induced fluorescence transient lift remote measurement of light use efficiency

Field experiments

  • Impact of cold stress on photosynthesis

  • Capsicum annuum

  • Ficus spec.

  • Lycopersicon esculentum

  • Persea americana

  • grass community dominated by Lolium spec.


Laser induced fluorescence transient lift remote measurement of light use efficiency

Predawn measurements on Lycopersicon esculentum


Laser induced fluorescence transient lift remote measurement of light use efficiency

Diurnal course: Lycopersicon esculentum


Laser induced fluorescence transient lift remote measurement of light use efficiency

Predawn measurements on Persea americana

2

1

4

3


Laser induced fluorescence transient lift remote measurement of light use efficiency

Diurnal cours: Persea americana

target 1

target 3


Laser induced fluorescence transient lift remote measurement of light use efficiency

Steady state fluorescence


Laser induced fluorescence transient lift remote measurement of light use efficiency

Summary II

  • The LIFT system can sensitively detect stress

  • correlated to ETR

  • In order to quantify photosynthetic CO2 uptake

  • the LIFT system has to be extended by measurements of:

  • - light

  • - stomatal conductance

  • - temperature


Laser induced fluorescence transient lift remote measurement of light use efficiency

Acknowledgement

Joe Berry

Zbigniew Kolber

Uwe Rascher

Denis Klimov

Larry Giles

Bob Haxo

Marie Curie Outgoing International Fellowships

(Nr: 041060 – LIFT)


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