Biosafety training
This presentation is the property of its rightful owner.
Sponsored Links
1 / 100

BIOSAFETY TRAINING PowerPoint PPT Presentation


  • 139 Views
  • Uploaded on
  • Presentation posted in: General

BIOSAFETY TRAINING. Tina Preseau* & Rita Toussaint-Archambault  * Office of Risk Management  Human Resources - Occupational Health Disability & Leave. COURSE OUTLINE. Introduction Laboratory Associated Infections Blood-borne Pathogens Classification of Biohazards

Download Presentation

BIOSAFETY TRAINING

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Biosafety training

BIOSAFETYTRAINING

Tina Preseau*

& Rita Toussaint-Archambault

*Office of Risk Management

Human Resources - Occupational Health Disability & Leave


Course outline

COURSE OUTLINE

  • Introduction

  • Laboratory Associated Infections

  • Blood-borne Pathogens

  • Classification of Biohazards

  • Infection/Biohazard Control

  • Spill Response

  • Biomedical Waste

  • Regulations

BIOSAFETY


Introduction

INTRODUCTION


What is a biohazard

WHAT IS A BIOHAZARD?

  • A potential hazard to humans, animals or the environment caused by a biological organism, or by material produced by such an organism

  • Examples:

  • Viruses, bacteria, fungi, and parasites and their product.

  • Blood and body fluids, as well as tissues from humans and animals.

  • Transformed cell lines and certain types of nucleic acids .


What is biosafety

WHAT IS BIOSAFETY?

  • Measures employed when handling biohazardous materials to avoid infecting oneself, others or the environment.

  • Achieved through;

    • Administrative Controls

    • Engineering Controls

    • Personal Protective Equipment

    • Practices and Procedures


What is biosecurity

WHAT IS BIOSECURITY?

  • Measures employed to protect biohazardous materials, or critical relevant information, against theft or diversion by those who intend to pursue intentional misuse.

  • Achieved through;

    • Physical barriers

    • Psychological barriers

    • Monitoring Activities

    • Personnel Clearance


Who are the stakeholders

WHO ARE THE STAKEHOLDERS?

EXTERNALLY

  • Public Health Agency of Canada

  • Canadian Food Inspection Agency

  • Environment Canada

  • Transport Canada

  • Ontario Ministry of Labour

  • Emergency Response Personnel

  • Suppliers & Contractors

  • Community

INTERNALLY

  • Vice-President (Research)

  • Committees

  • University Services (ORM, HR, PRS, PS)

  • Deans, Chairs, Principal Investigators, Employees, Students

  • Manager of Biological Containment Suite

ACCOUNTABILITY


Key services

KEY SERVICES

  • Office of Risk Management

    • Training

    • Interface with Regulatory Bodies

    • Biosafety Program

certifications

training

procedures

inspections

contingency planning

accident/incident follow-up


Key services1

KEY SERVICES

  • HR (Occupational Health, Disability and Leave)

    • Medical surveillance

    • Immunizations

    • Medical Follow-up

    • Interface with Workplace Safety and Insurance Board


Why are we concerned

WHY ARE WE CONCERNED?

  • Potential for acquiring a laboratory-associated infection (LAI)

  • Contamination of the environment

  • Contamination of research

  • Public perception


Laboratory associated infections

LABORATORY ASSOCIATED INFECTIONS


Laboratory associated infections1

LABORATORY ASSOCIATED INFECTIONS

  • Percutaneous inoculation

  • Inhalation of aerosols

  • Contact of mucous membranes

  • Ingestion

Infection Source

  • Cultures and stocks

  • Research animals

  • Specimens

  • Items contaminated with above

Susceptible Host

Route of Transmission

  • Immune system

  • Vaccination status

  • Age


Biosafety training

LAIS

  • Only 20% causative or defined event

    • 80% of which are caused by human factors

    • 20% are caused by equipment failure

  • Top 4 accidents resulting in infection

    • Spillages & splashes

    • Needle and syringe

    • Sharp object, broken glass

    • Bite or scratch from animals or ectoparasites

http://www.weizmann.ac.il/safety/bio2.html


Biosafety training

LAIS


Blood borne pathogens

BLOOD-BORNE PATHOGENS


Bloodborne pathogens bbp

BLOODBORNE PATHOGENS (BBP)

  • Sources

    • Blood

    • Semen

    • Vaginal Secretions

    • Other Bodily Fluids

      Cerebrospinal

      Amniotic

      Synovial

    • Tissue Cultures

    • Organ Cultures

    • Infected Experimental Animals


Risk of exposure

RISK OF EXPOSURE

  • Pathogen involved

  • Type of body fluid

  • Route of exposure

  • Duration of exposure

  • Volume of blood involved in exposure

  • Concentration of virus at time of exposure

  • PPE worn


Specific examples of bbps

SPECIFIC EXAMPLES OF BBPS

Hepatitis B

Hepatitis C

HIV


Issues to consider

ISSUES TO CONSIDER

  • Symptoms

  • Mode of transmission

  • Incubation period

  • Survival outside host

  • Communicability

  • Immunization

  • Prophylaxis / Treatment


If an exposure occurs

IF AN EXPOSURE OCCURS

  • Initiate first aid

  • Notify your supervisor / designated person

  • Report to hospital emergency department or University’s Health Services

  • Report incident to OHDL

    Occupational Health, Disability and Leave Office telephone ext. 1472 http://www.rh.uottawa.ca/00_main/index_f.asp


Universal precautions

UNIVERSAL PRECAUTIONS

  • Minimum standard of practice for preventing the transmission of BBP includes:

  • Education

  • Hand washing

  • Wearing protective barriers

  • Use safe work practices

If samples cannot be guaranteed non-infective …… treat as infectious!


Biohazard classification

BIOHAZARD CLASSIFICATION


Biohazard classification1

BIOHAZARD CLASSIFICATION

  • Conventional Agents

  • Recombinant DNA

  • Tissue Culture

  • Animal Work

  • Anatomical Specimens

  • Unconventional Agents

Class D, division 3 of WHMIS

(Poisonous and Infectious Material - Biohazardous Infectious Material)


Biohazard classification2

BIOHAZARD CLASSIFICATION

  • Organisms are categorized into a group base on the particular characteristics of each organism, such as

    • Pathogenicity

    • Infectious dose

    • Mode of transmission

    • Host Range

    • Availability of effective preventive measures

    • Availability of effective treatment


Biohazard classification3

BIOHAZARD CLASSIFICATION

  • Organisms are categorized base on the measuresrequired for handling each organism safely in a laboratory setting, such as

    • Engineering Requirements

    • Operational Requirements

    • Technical Requirements

    • Physical Requirements


Conventional agents

CONVENTIONAL AGENTS

Unlikely to cause disease in healthy workers or animals

Rarely cause serious human or animal disease

May cause serious disease

Likely to cause very serious disease


Recombinant dna

RECOMBINANT DNA

  • Genetic Engineering = in vitro incorporation of genetic material from one cell into another

  • In Canada the level of risk depends on source of DNA, vector and host.

  • The Office of Risk Management will assist the investigator in this determination.


Tissue culture

TISSUE CULTURE

  • Have the potential to contain pathogenic organisms

  • In general;

    Human & non-human primate, and mycoplasma-containing cell lines Level 2

    OthersLevel 1

A detailed risk assessment should be undertaken when using a new cell line.


Animal work

ANIMAL WORK

  • Animals can harbour infectious organisms (naturally or introduced)

  • Level dependent on type of work being conducted.

  • Special Animal Care training is required for all personnel working with animals.

  • All work involving animal use must receive prior approval from the Animal Care Committee


Anatomical specimens

ANATOMICAL SPECIMENS

  • All specimens should be considered infectious due to potential presence of infectious agents

  • Important to consider the type of specimen

    • blood, organs, tissues

    • Spinal sample, brain tissue

    • From infectious patient

  • In general Level 2 but it depends on the nature of the work.


Unconventional pathogens

UNCONVENTIONAL PATHOGENS

  • TSE prion diseases; lethal transmissible neurodegenerative conditions

    • Creutzfeld-Jakob disease, Variant C-J Disease, Mad Cow Disease, Scrapie, Chronic Wasting Disease

  • Resistant to destruction by procedures that normally inactivate viruses.

  • Contact ORM to assess requirements (containment, procedures, waste disposal, etc.)


Where can we find these

WHERE CAN WE FIND THESE?


Infection biohazard control

INFECTION/BIOHAZARD CONTROL


Infection biohazard control1

INFECTION/BIOHAZARD CONTROL

  • Administrative Controls

  • Engineering Controls

  • Personal Protective Equipment

  • Practices and Procedures


Administrative controls

ADMINISTRATIVE CONTROLS

  • Program based, information and methods to minimize risk of exposure:

  • Risk assessment

  • Medical Surveillance

  • Training/Education

  • Resources

  • Inspections

  • Signs & Labeling


Administrative controls1

ADMINISTRATIVE CONTROLS

Risk Assessment

  • Will determine type of containment, procedures, and safety equipment required

  • Responsibility of users, additional assistance is available from the ORM

  • Consider areas such as; experimental design, procedures to be employed and personal experience/knowledge, etc.

  • Know and understand the various characteristics of the agent(s) you are working with. (Material Safety Data Sheets and suppliers or manufacturers)


Administrative controls2

ADMINISTRATIVE CONTROLS

Medical Surveillance

Training & Education

  • WHMIS

  • Lab specific policies and procedures

  • Biosafety training

    Resources

  • ORM web site, Biosafety page

  • Faculty web sites

  • Biosafety Manual

  • Training Videos


Administrative controls3

ADMINISTRATIVE CONTROLS

Inspections

  • Routine self-inspections

  • Biosafety Inspection Checklist available on-line

  • In addition, ORM, EHSOs and OH&S will inspect labs to ensure compliance with regulations/ guidelines and provide feedback.


Administrative controls4

ADMINISTRATIVE CONTROLS

Signs & Labeling

  • Biohazard warning signs must be posted on doors to rooms where biohazardous materials are used.

  • Biohazard labels should be placed on containers, equipment and storage units used with biological agents.


Engineering controls

ENGINEERING CONTROLS

  • Technology based, reduce or eliminate exposure to hazards by changes at the source of the hazard.

  • Containment:

    • Types: Primary and Secondary

    • Containment levels


Primary containment

PRIMARY CONTAINMENT

  • First line of defence.

  • Ensures protection of personnel and immediate environment from exposure to the infectious agent.

  • ‘Protective envelope’ that encapsulates the infectious agent or animal.

    • Petrie dish, vial, stoppered bottle….

    • Biological safety cabinets, glove boxes and animal caging equipment, etc.

      Effectiveness of control is based on the integrity of the containment.


Secondary containment

SECONDARY CONTAINMENT

  • Protects the environment external to the laboratory from exposure

  • Includes facility design and operational practices


Containment level 1

CONTAINMENT LEVEL 1

  • Basic laboratory

  • Requires no special design features

  • Biosafety cabinets are not required and work may be performed on the open bench.


Containment level 2

CONTAINMENT LEVEL 2

  • Clinical, diagnostic, research and teaching facilities with level 2 agents.

  • Requires a class I or class II biological safety cabinet if any potential for aerosol or splash exists.

  • An emergency plan for handling spills must be developed.

  • Access should be controlled.


Biosafety training

CONTAINMENT LEVEL 3

  • Specialized design and construction

    • primary barriers to protect the individual

    • secondary barriers to protect the environment

  • All staff must undergo special training on the agents being used, PPE, equipment, waste management as well as practices and procedures above and beyond the scope of this course.


Containment level 4

CONTAINMENT LEVEL 4

  • Only one level 4 facility in Canada (Canadian Centre for Human and Animal Health in Winnipeg, Man.)

  • Design specifications are extremely stringent, worker is completely isolated from infectious material.


Biological safety cabinets

BIOLOGICAL SAFETY CABINETS

  • Effective means of primary physical containment for biological agents, especially when aerosols are generated.

  • HEPA filters remove particles (min 0.3 microns) with 99.97% efficiency.

  • There are 3 main classes of cabinets (I, II, III) which provide various levels of protection.


Biological safety cabinets1

BIOLOGICAL SAFETY CABINETS

  • Biological Safety Cabinet

  • HEPA filtered laminar air flow

  • Exhaust

  • Personnel, environment & product protection

  • Laminar flow hoods

  • Vertical or horizontal laminar flow

  • HEPA filtered air (intake only)

  • Product protection only

VS


Working safely in a bsc

WORKING SAFELY IN A BSC

Before using the cabinet:

  • Ensure BSC is certified

  • Turn off UV lamp; turn on fluorescent lamp

  • Disinfect work surfaces with appropriate disinfectant

  • Place essential items inside cabinet

  • Allow the blower to run for 5-10 min before work


Biosafety training

WORKING SAFELY IN A BSC

While using the cabinet:

  • Ensure material and equipment is placed near the back of the hood, especially aerosol-generating equipment. Do not block any vents

  • Use techniques that reduce splatter and aerosols.

  • General work flow should be from clean to contaminated areas

  • Minimize movement so as not to impede air flow

  • Open flame in BSC’s is controversial


Biosafety training

WORKING SAFELY IN A BSC


Biosafety training

WORKING SAFELY IN A BSC

After using the cabinet:

  • Leave blower on at least 5 minutes to purge cabinet

  • Remove and decontaminate equipment and materials

  • Disinfect cabinet surfaces

  • Turn off blower and fluorescent lamp, turn on UV lamp


Working safely in a bsc1

WORKING SAFELY IN A BSC

Maintenance:

  • Before and after each use - Work surfaces wiped down

  • Weekly- UV lamp should be wiped clean

  • Monthly - All vertical surfaces wiped down

  • Annually- UV lamp intensity verified

    - Decontamination with formaldehyde gas (ORM)

    - Certification (ORM)


Personal protective equipment ppe

PERSONAL PROTECTIVE EQUIPMENT (PPE)

  • PPE can become an important line of defence (last line of defense)

  • Responsibility of both the user and the supervisor to ensure that PPE is worn


Biosafety training

PPE

  • Criteria for consideration

    • Routes of exposure that need to be blocked

    • Degree of protection offered

    • Ease of use

  • Only effective if correctly selected, fitted, used and cared for, and the individual is trained

  • Ensure PPE is removed before leaving the lab


Biosafety training

PPE

Footwear

  • Closed toed shoes should always be worn. Booties are worn in some higher containment labs and animal facilities.

    Lab Coats/Gowns

  • Long-sleeved, knee length with snaps

  • Elastic cuffs

  • Back-closing gowns

  • Periodic cleaning required


Biosafety training

PPE

Gloves

  • Latex, nitrile & vinyl for work with biological agents.

  • Exam gloves should not be reused, change frequently. Utility gloves can be disinfected and reused if they show no sign of degradation.

  • Consider tensile characteristics, length of cuff.

  • Double gloving.

  • ORM can provide assistance finding an alternative for people with allergies.


Biosafety training

PPE

Eye & Face Protection

  • Goggles, safety glasses to protect the eyes

  • Full face shield to protect facial skin.

    Respirators

  • Only personnel who have been fit-tested and trained should wear respirators.


Practices and procedures

PRACTICES AND PROCEDURES

  • General Safety Guidelines

  • Good Microbiological Practice

  • Handwashing

  • Suspicious Packages

  • Specific Procedures

    • Centrifuges

    • Needles & Syringes and other sharps

    • Pipettes

    • Blenders, Grinders, Sonicators & Lyophilizers

    • Inoculation Loops

    • Cryostats


General laboratory safety guidelines

GENERAL LABORATORY SAFETY GUIDELINES

  • Mostly common sense, but you must understand the hazards you face in the laboratory and be adequately trained to deal with them.

  • Basic must be known for all labs.

b

i

o

s

a

f

e

t

y


Good microbiological practice gmp

GOOD MICROBIOLOGICAL PRACTICE (GMP)

  • Basic code of practice that should be applied to all types of work involving microorganisms.

  • Objectives:

    • prevent contamination of laboratory workers and the environment

    • prevent contamination of the experiment/samples

  • Application of aseptic technique, minimization of aerosols, contamination control, personal protection, emergency response


Handwashing

HANDWASHING

  • One of the single effective means of preventing infections if done properly and frequently

  • When to wash?

    • Before starting any manipulations

    • Before leaving the lab

    • When hands are obviously soiled

    • Before and after completing any task in a BSC

    • Every time gloves are removed

    • Before contact with one’s face or mouth

    • At the end of the day


Suspicious packages

SUSPICIOUS PACKAGES

Unopened

  • Do not open and do not shake

  • Place in secondary container or cover

  • Inform others of the situation

  • Clear the room and section off the area

  • All individuals who may have come into contact with the material must wash their hands

  • Call Protection Services and wait for their arrival

  • List all the individuals present in the room or area when the package arrived. Give this list to Protection Services for follow-up


Suspicious packages1

SUSPICIOUS PACKAGES

Opened

  • Contents Intact

  • Do not manipulate contents further

  • Cover the package

  • Inform others of the situation

  • Clear the room and section off the area

  • All individuals who may have come into contact with the material must wash their hands

  • Call Protection Services and wait for their arrival

  • List all the individuals present in the room or area when the package arrived. Give this list to Protection Services for follow-up


Suspicious packages2

SUSPICIOUS PACKAGES

  • Contents not intact (spilled)

  • Do not try to clean up the spill

  • Gently cover the spill

  • Inform others of the situation

  • All individuals who may have come into contact with the material must wash their hands

  • Call Protection Services

  • Remove heavily contaminated clothing (place in bag) and shower using soap and water

  • List all the individuals present in the room or area when the package arrived. Give this list to Protection Services for follow-up


Safe use of centrifuges

SAFE USE OF CENTRIFUGES

  • Before use

    • Stress lines? Overfilled? Balanced?

    • Caps or stoppers properly in place?

    • Run conditions achieved?

  • Use sealable buckets (safety cups) or sealed rotors

  • After run

    • Centrifuge completely stopped?

    • Spills or leaks?

    • Allow aerosols to settle (30 min) or open in a BSC.


Needles and syringes

Avoid use whenever possible

Use a BSC for all operations with infectious material

Fill syringes carefully

Shield needles when withdrawing from stoppers

Do not bend, shear or recap needles.

Dispose of all used needles/syringes in yellow sharps containers

NEEDLES AND SYRINGES


Pipettes

PIPETTES

  • Mouth pipetting is prohibited.

  • Never force fluids out.

  • To avoid splashes, allow discharge to run down dispense the receiving container wall.

  • Never mix material by suction and expulsion.

  • Reusable pipettes should be placed horizontally in a disinfectant filled pan.


Blenders grinders sonicators and lyophilizers

BLENDERS, GRINDERS, SONICATORS, AND LYOPHILIZERS

  • Operate in a BSC whenever possible. Allow aerosols to settle for 5 minutes before opening.

  • Safety Blender

    • Do not use glass blender jars

    • Decontaminate immediately after use

  • Lyophilizers

    • Use glassware designed for vacuum work, ensure there is no damage before using

    • All surfaces should be disinfected after use

    • Use vapour traps whenever possible


Inoculation loops

INOCULATION LOOPS

  • Sterilization in an open flame may create aerosols which may contain viable microorganisms.

  • Use a shielded electric incinerator

  • Shorter handles minimize vibrations

  • Disposable plastic loops are good alternatives


Cryostats

CRYOSTATS

  • Wear gloves during preparation of frozen sections and heavy gloves when accessing the cryostat.

  • Decontaminate frequently (100 or 70% Ethanol)


Spill response

SPILL RESPONSE


Spills

SPILLS

  • Spill response will vary depending on:

    • What was spilled?

    • How much was spilled?

    • Where was the spill?

    • What is the potential for release to the environment?

  • Spills should be cleaned up immediately (unless an aerosol was generated), to ensure proper decontamination.

  • Ensure appropriate PPE is worn and clean-up equipment is readily available.


Spills general clean up

SPILLS-GENERAL CLEAN-UP

  • Cover spill area with absorbent material

  • Soak the spill area with an appropriate disinfectant (i.e. 10% bleach)

  • Pour disinfectant from the outside of the absorbent material towards the inside

  • Ensure any broken glass is picked up (with forceps!) and placed in a sharps container

  • Leave on for 20 to 30 minutes

  • Wipe up with absorbent material

  • Waste should be disposed in appropriate biohazard bags and where possible autoclaved


Spills special cases

SPILLS-SPECIAL CASES

  • Within a Centrifuge

  • Within a BSC

  • Open Areas (lab, during transport)

  • The spill response plan template is available at (http://www.uottawa.ca/services/ehss/SPILLRESPONSEPLAN.pdf)


Spills1

SPILLS

  • All users of biological materials should be familiar with the spill clean-up procedures.

  • All spills are to be reported ASAP to the lab supervisor and ORM.

  • Additional assistance is available from:

    • ORM x 5892

    • Your departmental safety officer

    • ERT x 5411 (through Protection Services)


Biomedical waste

BIOMEDICAL WASTE


Decontamination disinfection and sterilization

DECONTAMINATION, DISINFECTION, AND STERILIZATION

  • Decontamination: Free of contamination, the destruction of microorganisms to a lower level such that it removes danger of infection to individuals.

  • Sterilization: The complete destruction of all viable microorganisms.

  • Disinfection: Use of agents (physical or chemical) to destroy harmful organisms on inanimate objects (not necessarily all organisms)


Decontamination physical

DECONTAMINATION: PHYSICAL

  • Heat:

    • Autoclaving (most practical and recommended)

    • Incineration (for disposal of sharps and tissues)

  • Irradiation:

    • UV light (wavelength of 253 nm is germicidal)

    • Gamma (disrupts DNA and RNA)

  • Filtration

    • HEPA (biological safety cabinets, ventilation)


Autoclaves

AUTOCLAVES

Items that CAN be autoclaved:

  • Cultures and stocks of infectious material

  • Culture dishes and related devices

  • Discarded live and attenuated vaccines

  • Contaminated solid items (petrie dishes, eppendorf tips, pipettes, gloves, paper towels)


Autoclaves1

AUTOCLAVES

Items that CAN NOT be autoclaved:

  • chemicals (flammables, oxidizers, phenols, acids, alkalides)

  • chemotherapeutic or radioactive waste

  • Bleach (or other chlorinated products)

  • certain kinds of plastics

  • sharps (not at the University of Ottawa)


Autoclaves2

AUTOCLAVES

Preparation of waste:

  • Use only approved autoclave bags

  • Do not overfill autoclave bags

  • Separate material for re-use from that which will be disposed and dry from liquid material

  • If outside of bag is contaminated, double bag

  • All flasks containing biological material should be capped with aluminum foil

  • Ensure items are labeled with contact information


Safe use of autoclaves

SAFE USE OF AUTOCLAVES

  • Many autoclaves are now run by dedicated staff, however, if you are operating an autoclave;

    • Learn how to use it!

    • Ensure PPE is worn

    • Recognize acceptable material and packaging

    • Proper loading and unloading

  • All users/operators must take the autoclave training


Decontamination chemical

DECONTAMINATION: CHEMICAL

  • Generally for disinfection rather than sterilization

  • Choice depends on;

    • Type of material to be disinfected

    • Organic load

    • Chemical characteristics

  • Most common are chlorine compounds and alcohols (broad range)


What to use for my agent

WHAT TO USE FOR MY AGENT?

Viruses

Enveloped (HIV, Herpes)

  • 2% domestic bleach

  • 75% Ethanol

  • Quaternary ammonia

  • 6% formulated Hydrogen peroxide*

    Non enveloped(Hepatitis, Adenovirus)

  • 10% domestic bleach

  • 6% formulated Hydrogen peroxide*

  • Gluteraldehyde

  • Formaldehyde

Vegetative bacteria(E.coli, Staph)

  • 2% domestic bleach

  • 75% Ethanol

  • Quaternary ammonia

  • 6% formulated Hydrogen peroxide

    Mycobacteria and fungi

  • 10% domestic bleach

  • 75% Ethanol

  • Phenolic compounds

  • 6% formulated Hydrogen peroxide

    Spore forming bacteria(Bacillus)

  • 10% domestic bleach

  • Gluteraldehyde

  • Formaldehyde

  • 6% formulated Hydrogen peroxide


Waste management

WASTE MANAGEMENT

Discarded biological material from teaching, clinical and research laboratories and operations is biomedical waste. Biomedical waste includes but is not limited to;

  • Animal waste

  • Biological laboratory waste

  • Human anatomical waste

  • Human blood and body fluid waste

  • Sharps


Waste management1

WASTE MANAGEMENT

  • All biological waste should be decontaminated prior to disposal (including level 1 agents).

  • Treated waste is no longer considered ‘biomedical’ (i.e. microbiological waste, blood and bodily fluid waste) and can be disposed in the regular waste stream.

  • Any waste that cannot be treated (i.e. sharps, carcasses, tissues and body parts) remains biomedical waste and must be incinerated off site.


Waste disposal

WASTE DISPOSAL

Biomedical Waste (untreated)


Waste disposal1

WASTE DISPOSAL

Biomedical Waste (treated)

*

In compliance with Sewer use by-laws

With H2O 1:10


Special waste

SPECIAL WASTE

  • EtBr

  • Toxins

  • Recombinant DNA

  • Contact ORM


Regulations

REGULATIONS


Key regulated activities

KEY REGULATED ACTIVITIES

  • Purchasing & Receiving of Biological Agents

    • PHAC, CFIA, Environment Canada

    • Inventory Records

  • Transportation/Transfer

    • Transport Canada- TDG

  • All Agencies (provincial and federal) emphasize and expect Biosecurity


  • Purchasing

    PURCHASING

    • Importation permits required by Public Health Agency Canada or CFIA for certain agents

    • US restrictions

    • Ensure you meet all criteria and have all pertinent documentation


    Inventory

    INVENTORY

    • What material is presently being used and/or stored

      • Location

      • Expiry date

      • Use log book for remaining amount

      • MSDS’s

    • Mandatory


    Shipping and receiving

    SHIPPING AND RECEIVING

    • Transportation of Dangerous Goods Act: Class 6.2 of (Infectious Substances)

    • PHAC/CFIA restrictions

    • Ensure;

      • Proper classification

      • Proper packaging

      • Proper labeling

      • Proper documentation

      • Import/Export Permits


    Transportation of dangerous goods

    TRANSPORTATION OF DANGEROUS GOODS

    • Pre-approved

    • Authorized Individuals

    • Lead time (International Regulations….)

    • Appropriate Scheduling (Holidays, Weekends)

    • Transportation within the building

    • Between lab to lab

    • Colleague to Colleague

    • Between Institutions


    Transportation

    TRANSPORTATION

    • Important Considerations:

      • does material need to be transported at all

      • packaging requirements

      • means and route of transportation

      • regulatory requirements

    • Between lab transfers - 4 sided cart, sealed primary container, secondary container, low traffic route.

    • Off Campus transfers – consult ORM


    The bottom line

    THE BOTTOM LINE

    • If you are not careful and diligent with biological agents you risk:

      • Infecting yourself, others or the environment

      • Contaminating your research

      • Having Public Health Agency of Canada, Canadian Food Inspection Agency, Ministry of the Environment or Transport Canada after you


    Conclusions

    CONCLUSIONS

    • Biohazards - microorganisms, blood and body fluids, tissues and tissue culture

    • Biosafety - ensuring that individuals and the environment are not infected

    • Biosecurity - used in the context of protecting dangerous pathogens and toxins against intentional removal

    • Everyone within the University community is responsible

    • With proper knowledge, planning and care, a biological exposure is avoidable.


    Biosafety website

    BIOSAFETY WEBSITE

    http://www.uottawa.ca/services/ehss/biosafety.htm


  • Login