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Thraustychrids

Transcriptomic characterization of Quahog Parasite Unknown (QPX) and the effects of temperature on differentially expressed genes. Annie Page-Karjian, Maya Groner, Jamie Sziklay, Ana Elisa Garcia, Ashton Griffin, Gregor-Fausto Siegmund, Sonia Singhal, Steven Roberts. Thraustychrids.

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Thraustychrids

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  1. Transcriptomic characterization of Quahog Parasite Unknown (QPX) and the effects of temperature on differentially expressed genes Annie Page-Karjian, Maya Groner, Jamie Sziklay, Ana Elisa Garcia, Ashton Griffin, Gregor-Fausto Siegmund, Sonia Singhal, Steven Roberts

  2. Thraustychrids - Unicellular protists - Poorly described taxonomic group - Mostly saprophytes, but includes some highly virulent pathogens

  3. QPX Pathology - Massive inflammatory response - Swelling and nodules mantle and labial palps - Slower growth

  4. Taken from Perrigault2010 [[[FULL REF]]]

  5. Host Environment Disease Pathogen

  6. Brothers et al 2000

  7. Objectives of our study 1. Sequence and annotate the QPX genome and transcriptome 2. Evaluate the effects of temperature on the QPX transcriptome 3. Identify enriched biological function in enriched genes

  8. 10 ˚C 21 ˚C 45,082,610 reads 30,035,397 reads Illumina Sequencing De novo assembly 11,280 contigs RNA seq 2x Enrichment analysis 613 genes are differentially expressed!

  9. Results: Transcriptome characterization

  10. Results: Transcriptome characterization All genes within the QPX transcriptome which showed a GO Slim stress response: Talk about the chart, friend!

  11. Objectives of our study 1. Sequence and annotate the QPX genome and transcriptome 2. Evaluate the effects of temperature on the QPX transcriptome 3. Identify enriched biological function in enriched genes

  12. Results: Differential gene expression

  13. Enrichment analysis

  14. Objectives of our study 1. Sequence and annotate the QPX genome and transcriptome 2. Evaluate the effects of temperature on the QPX transcriptome 3. Identify enriched biological function in enriched genes

  15. Genetic connectionsbetween processes Developmental processes Protein metabolism Death Cell adhesion DNA metabolism Transport Stress response Cell cycle and proliferation RNA metabolism Cell organization and biogenesis Signal transduction

  16. Results: Genome characterization

  17. Discussion

  18. Acknowledgements

  19. Questions?

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