1 / 1

c/d-rRNA locus (chromsome 5/6)

4x10 6 . 1,2x10 6 . Supp Figure 1. Generation of K173 cl1 -GFP-Luc schiz-a ( gfp-luc integration by single cross-over). A. gfp-luc. K. construct pL0028. SM. 3' ssu. pUC. ama1. 3’dhfr. 5' ssu. c/d-rRNA locus (chromsome 5/6). c/d -ets. ssu. lsu. 5.8S. gfp-luc.

drea
Download Presentation

c/d-rRNA locus (chromsome 5/6)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 4x106 1,2x106 Supp Figure 1 Generation of K173cl1-GFP-Lucschiz-a (gfp-luc integration by single cross-over) A gfp-luc K construct pL0028 SM 3'ssu pUC ama1 3’dhfr 5'ssu c/d-rRNA locus (chromsome 5/6) c/d -ets ssu lsu 5.8S gfp-luc c/d-rRNA locus after integration SM c/d -ets ssu’ ama1 3’dhfr ssu B Generation of K173cl1-GFP-Lucschiz-b (gfp-luc integration by double cross-over) gfp-luc S S construct pL1403 3'dhfr ama1 5‘ssu 3’ssu c/d-rRNA locus (chromsome 5/6) c/d -ets ssu lsu 5.8S Δssu Δssu gfp-luc c/d-rRNA locus after integration c/d -ets ama1 3’dhfr Southern analysis of separated chromosomes (Probe: ANKA_3’-UTR dhfr/ts) C K173cl1 -GFP-Lucschiz-b K173cl1 -GFP-Lucschiz-a ANKAwt ANKAwt K173cl1 - chr 7 - chr 7 chr 5/6 - chr 5/6 - chr 7 - chr 7 - D K173cl1 GFP-Lucschiz-b K173cl1 GFP-Lucschiz-a

More Related