Optimization of sanitizing process using heat against bacteria asaia bogorensis
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INSTITUTE OF CHEMICAL TECHNOLOGY , PRAGUE Department of Food Preservation and Meat Technology. Optimization of Sanitizing Process Using Heat Against Bacteria Asaia bogorensis. Petra Klodnerová, MSc Supervisor: Miroslav Čeřovský, MSc, PhD. Asaia bogorensis.

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Optimization of Sanitizing Process Using Heat Against Bacteria Asaia bogorensis

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INSTITUTE OF CHEMICAL TECHNOLOGY, PRAGUE

Department of Food Preservation and Meat Technology

Optimization of Sanitizing Process Using Heat Against Bacteria Asaia bogorensis

Petra Klodnerová, MSc

Supervisor: Miroslav Čeřovský, MSc, PhD


Asaia bogorensis

Fig. 2: Microscopic picture of cells.

Fig. 1: Colonies on PCA Agar.


Aim of Research Heat Resistance of A. bogorensis

  • technological problem

    • Asaia bogorensis = new unusual spoilage organism of still fruit-flavored bottled water

  • sanitizing process optimalization, using heat

    • find D and zvalue


3.5

3

2.5

2

Log D

1.5

1

z

0.5

7

Temperature

6

5

4

Log N

3

2

D

1

0 2 4 6 8 10 12 14

Time

Thermal Inactivation ParametersD and z value

Fig.3: Survivor curve

Fig.4: Thermal-death-time curve


10 mm

12 mm

1.9 mm

Holder of

capillary tube

41 mm

135 mm

Teflon

piston

1.5 mm

Metal capillary tube

Silicon gasket

Methods and Instruments

Fig. 6: Equipment for

capillary tube method

Fig. 5: TDT tube method


Experimental Procedure

sample preparation – suspension

cultivated 96 hr, 30 °C

TDT and capillary tube filling with sample

tube submersion in heating water bath

tube immersion in ice-water bath

heated samplemicrobial analysis

construction of microbial survivor

curve and thermal-death-time curve

D and zvalue


D and z Values of Asaia bogorensis in Nutrient Broth


Comparison of TDT Tube Method and Capillary Tube Method


D50 Value of Asaia bogorensis in McIlvaine Buffer


CONCLUSIONS

  • A. bogorensisis more heat resistant after cultivation at 30 °C than at 25 °C.

  • A. bogorensis is sensitive to relatively low temperatures (D55= 0,6 min).

  • Heat resistance of A. bogorensis at 50 °C is the least at pH 8.

  • Possibilityof heat treatment usage for optimalization of sanitizing process, whereas thermally sensitive components of equipment are not damaged.


Thank You for Your Attention


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