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GE2678 GATGCAGCAGAT tCTaGA TGATCC GE2679 ATCA tCTaGA ATCTGCTGCATCTGC

Xba I. Nhe I. 1a. GE2678. GE2680. GE2679. GE2681. 1c. GE2676. GE2674. GE2677. GE2675. 575Q/576L (1a) 571Q/572L (1c). 366I/367L (1a) 364I/365L (1c). GE2678 GATGCAGCAGAT tCTaGA TGATCC GE2679 ATCA tCTaGA ATCTGCTGCATCTGC GE2680 CGCTGCA GCTaGC ACATTACAAG

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GE2678 GATGCAGCAGAT tCTaGA TGATCC GE2679 ATCA tCTaGA ATCTGCTGCATCTGC

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  1. XbaI NheI 1a GE2678 GE2680 GE2679 GE2681 1c GE2676 GE2674 GE2677 GE2675 575Q/576L (1a) 571Q/572L (1c) 366I/367L (1a) 364I/365L (1c) GE2678GATGCAGCAGATtCTaGATGATCC GE2679ATCAtCTaGAATCTGCTGCATCTGC GE2680CGCTGCAGCTaGCACATTACAAG GE2681GTAATGTGCTaGCTGCAGCGC GE2674GCAGTTCCAGTACATtCTaGATGACC GE2675TCAtCTaGAATGTACTGGAACTGCTG GE2676TCCTGCAGCTaGCCCACTTCC GE2677AAGTGGGCTaGCTGCAGGAC Supplementary Fig. S1 The experimental strategies used for preparation of the cDNAs encoding CPT1 chimeras made between 1a and 1c isozymes, and the nucleotide sequences of the primers used are shown. To enable chimeric constructions, artificial restriction sites of XbaI and NheI were introduced into the cDNA encoding CPT1 isozymes without changing their original amino acid sequences.

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