Bacteria. Bacteria review one-celled prokaryotes reproduce by mitosis binary fission rapid growth generation every ~20 minutes 10 8 (100 million) colony overnight! dominant form of life on Earth incredibly diverse. Transformation. promiscuous ! ?. Bacteria are opportunists
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mix heat-killed pathogenic & non-pathogenic
How have theselittle guys gotten to be so diverse??
gene we want
cut plasmid DNA
insert “gene we want” into plasmid...“glue” together
Madam I’m Adam
Able was I ere I saw Elba
a man, a plan, a canal, Panama
Was it a bar or a bat I saw?
go hang a salami I’m a lasagna hog
Madam I’m AdamRestriction enzymes
“new” protein from organism
ex: human insulin from bacteria
How can bacteria read human DNA?Why mix genes together?
human insulin gene in bacteria
DNA RNA protein trait