M icroPET : Radiotracer I maging of Rodents and Non- H uman Primates

1. MicroPET: Radiotracer Imaging of Rodents and Non-Human Primates Alexander K. Converse, PhD University of Wisconsin–Madison Waisman Center - Brain Imaging Core Town Hall - Thursday 2 February 2012

2. Radioactive Decay & Positron – Electron Annihilation

3. Positron Emission Tomography

4. UW-Madison PET

5. 3 uL 6 uL 2 uL + MR Animal Brain PET Scanners 3 uL

6. PHARMACOKINETIC MODELING Fig. 25: QUANTIFICATION: Logan determination of binding of a dopamine D2 tracer5 Fig. 23. PARAMETER ESTIMATION: Time activity curves from a multiple injection study of a dopamine D2 receptor ligand in rhesus15 Fig. 24 DOPAMINE RELEASE: Time activity curves of a dopamine D2 receptor tracer in response to amphetamine5 Fig. 26 BLOOD VOLUME: Carbon monoxide imaging of hemoglobin in rat12

7. INFLAMMATION Fig. 20 MULTIPLE SCLEROSIS: Microglial cell activation in white matter in response to zymosan in rat11 Fig. 19 ASTHMA: Glucose metabolism in inflammation in a rat lung10

8. PAIN Fig. 22 AVIAN VETERINARY ANALGESIA: Increased glucose metabolism in response to experimental arthritis in parrot6 Fig. 21 OPIOID RECEPTORS: Kappa opioid receptor availability in parrot brain6

9. PARKINSON’S DISEASE Fig. 16 NEURODEGENERATION: Rat model of striatal neurodegneration for pre- (left) and post- (right) stem cell treatment (first image from the microPET P4, 2002) Fig. 15 STEM CELL THERAPY: Dopamine synthesis in a rhesus model pre- and post- unilateral lesion13 Fig. 17 GENE THERAPY: Dopamine synthesis pre- and post- lentiviral delivery of GDNF in a unilateral lesion rhesus model8 Fig. 18 L-DOPA: Recovery of AAAD activity in a rhesus model7

10. MOOD DISORDERS Fig. 7 CHILDHOOD ANXIETY: Dopamine D2 receptors in rhesus1 Fig. 8 CHILDHOOD ANXIETY: Glucose metabolism in rhesus9 Fig. 9 SEXUAL BEHAVIOR: Glucose metabolism in female marmosets

11. ADDICTIVE BEHAVIORS Fig. 12 PRENATAL ALCOHOL EXPOSURE: Image of dopamine transporter in rhesus and time activity curves4 Fig. 10 PRENATAL ALCOHOL EXPOSURE: glucose metabolism alterations due to a reversal task in rhesus3 Fig. 11 PRENATAL ALCOHOL EXPOSURE: Serotonin 1A receptors in rhesus2 Fig. 13 AMPHETAMINE: Blood flow alteration correlated with dopamine release in rhesus5 Fig. 14: EATING BEHAVIORS Dopamine D2 receptor response to deep brain stimulation in rhesus14

12. Radiotracers Used with the Waisman Center microPET

14. Design Logistics Radiochemistry Scanner Analysis PET

15. Alex Converse Director, MicroPET Imaging Waisman Center Brain Imaging Core akconverse@wisc.edu