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BIOL 4014 Microbiology Fall 2005

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BIOL 4014 Microbiology Fall 2005 Mon & Wed, Lecture 8 am LSW 444; Lab 9-10:50 am or 3-4:50 pm, LSW 546 Instructor: Dr. David F. Gilmore Office: LSE 418 Phone 972-3632

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BIOL 4014 Microbiology Fall 2005

Mon & Wed, Lecture 8 am LSW 444; Lab 9-10:50 am or 3-4:50 pm, LSW 546Instructor: Dr. David F. Gilmore

Office: LSE 418 Phone 972-3632

Email: [email protected] Web: http://www.clt.astate.edu/dgilmore Tentative Office hours: Tuesday & Thursday 10:30- noon,

Friday 8:30- 10:30; other times by appt.Text: Microbiology by R. Bauman.

Lab text: A Photographic Atlas for the Microbiology Lab, 3rd ed, Leboffe and Pierce.

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Integrated Lab/Lecture course

  • Match up of lecture topics and Lab exercises
  • Additional time for discussion of lecture material, lecture exams.
  • Avoid “is this lab or lecture?” thinking.
  • Powerpoint lectures
  • Each slide numbered for your reference
  • Listen to ME, take smart notes
  • Powerpoints will be posted following class
  • Stuff
  • Schedules may change, more likely in lab
  • Alteration in points for Lab assignments
  • Don’t be a stranger.
  • Please no cell phone use or ringing in class.
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Grading

  • Four regular exams, 100 points each
  • Final (fifth) Exam, 100 points. Comprehensive final optional.
  • Lab assignments totalling 450 points
    • Two or Three Lab Reports
    • One Lab Exam and one Lab Final
    • One or two Unknown identifications
  • Attendance in lecture: 50 points.
  • Total 1000 points

Student email, my web page

Student ID numbers

Cheating

Roll call/ about attendance

microbiology definitions
Microbiology: Definitions
  • Chapter 1 in text
  • Microbiology: study of living things too small to be seen w/o a microscope.
    • What’s life?
      • Highly organized, self replicating, self-adjusting, capable of evolving, can obtain energy
    • Are all microbes that small?
      • Epulopiscium and Thiomargarita: visibly large bacteria
classification of microbes
Classification of Microbes
  • Three domains
    • Eubacteria: prokaryotic cell structure
    • Archaebacteria: prokaryotes, but different
    • Eukaryotes: 4 kingdoms
      • Plants, animals, fungi, and protists.
  • What are microbiologists interested in?
    • Eubacteria and archaebacteria for sure.
    • Eukaryotes like fungi and protists
    • Animals (parasitic worms) but not really plants.
    • What’s missing?
    • VIRUSES!
divisions of microbiology
Divisions of Microbiology
  • By critter type:
    • Bacteriology, virology, mycology
    • Parasitology (includes protozoa, fungi, worms)
  • Other divisions:
    • Pathogenic microbiology, Immunology, molecular biology, microbial ecology
    • Applied microbiology: water treatment, natural products, food microbiology, environmental microbiology
history ancient knowledge
History: Ancient knowledge
  • Recognition of Immunity:
    • Variolation and protection from infection
      • Intentional contact with minor form of smallpox
    • Edward Jenner and cowpox
      • Milkmaids catch cowpox, seem to be immune to smallpox.
  • Contagion: disease can be spread by contact.
      • Exclusion of lepers; burning of plague victims
      • Catapulting of disease victims into castles during seige
satire on jenner and vaccination
Satire on Jenner and vaccination

encarta.msn.com/.../ Vaccination_with_Cowpox.html

history continued
History continued
  • Microbiology as a biological science
    • Robert Hooke, 1665, discovery of cells
    • Antony van Leeuwenhoek, father of microbiology
      • Dutch amateur lens grinder
      • First person to see microbes, late 1600s
    • Mid 1800s, microbes taken more seriously and studied using the scientific method

micro.magnet.fsu.edu/.../ introduction.html

history the golden age
History: the Golden Age
  • From about 1850 to start of 20th century
    • Pasteur lays to rest the idea of spontaneous generation
    • Pasteur shows fermentation associated with life
      • Prevents unwanted fermentation by Pasteurization
      • Saves the French wine industry
    • Germ theory of disease: sicknesses caused by microbes
    • Robert Koch: lab techniques like agar, staining
      • Koch’s postulates: how to link a microbe w/ a disease.
    • Semmelweis and handwashing
    • Lister and aseptic surgery
    • Ehrlich and antimicrobials
20 th century microbiology
20th Century Microbiology
  • Molecular biology
      • Use of microbes as model systems for study
      • Study of DNA, proteins synthesis
      • Tools and processes for recombinant DNA
  • Applied microbiology
      • Food industry
      • Water and sewage treatment
      • Bioremediation
  • Medicine
    • Emerging diseases; antibiotic resistance
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Why Study Microbes?

  • Major impact on health
    • Responsible for disease in humans, animals, plants
  • Major impact on environment
    • Major decomposers
    • Nutrient cycling, elemental cycling
  • Microbes are talented
    • Live under extreme conditions
    • Protect against disease
    • Eat oil, toxic waste (bioremediation)
    • Make plastic
    • Spoil food, make food
    • Use light, produce light
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