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Biology 1060 Chapter 20

Biology 1060 Chapter 20. DNA Technology and Genomics. Manipulating Genomes. Define genetic engineering, recombinant DNA, biotechnology Define gene clone and plasmid Describe their usefulness Discuss the utility of restriction enzymes in vivo and in vitro. Eukaryotic Genes Cloning.

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Biology 1060 Chapter 20

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  1. Biology 1060Chapter 20 DNA Technology and Genomics

  2. Manipulating Genomes • Define genetic engineering, recombinant DNA, biotechnology • Define gene clone and plasmid • Describe their usefulness • Discuss the utility of restriction enzymes in vivo and in vitro

  3. Eukaryotic Genes Cloning • Describe the use of resistance to antibiotics and enzymes to hydrolyze specific substrates in gene cloning • Describe the use of a nucleic acid probe and denaturation in nucleic acid hybridization • Define genome library • Describe its possibilities

  4. Eukaryotic Gene Cloning continued • Compare and contrast a genome library and cDNA library • Describe the prolems of attempting to express eukaryotic genes in a prokaryote • Describe how these problems have been overcome • Describe the benefits and drawbacks of polymerase chain reaction

  5. Restriction Fragments • Describe the benefits of restriction fragment analyses • Describe the processes of gel electrophoresis and Southern blotting • Describe the information obtained from these techniques • Describe restriction fragment length polymorphisms • Describe their uses in finding genetic markers

  6. Genome Mapping • Describe the three techniques used to uncover an entire genome • Discuss the significance of the “most ambitious mapping project to date” and other genome sequences

  7. Genomics • Describe the value of genomics • Discuss the reasons the human genome has only 25% to 50% of the previously estimated size • Discuss two techniques used to determine a functional gene • Scientific Inquiry p. 410

  8. Genomics • Describe how genomics help discover how genes interact to produce and maintain a functioning organism • Describe the usefulness of genome comparisons to evolutionary studies • Discuss proteomes and its value to humans

  9. Applications • Discuss the medical applications of DNA technology • Discuss ethical issues associated with gene therapy • Describe how DNA technology can be used by pharmaceutical scientists • Discuss DNA fingerprinting as used in forensics

  10. Agriculture and DNA Technology • Describe the usefulness of transgenic animals • Describe the usefulness of transgenic plants

  11. Safety and Ethics • Describe the safety concerns regarding genetic modifications to organisms • Discuss ethical issues regarding genetic modifications • Evolution Connection p. 410 • Science, Technology, and Society p. 410

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