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A publikus adatbázisból hiányzó fehérjék azonosítási lehetõségei

A publikus adatbázisból hiányzó fehérjék azonosítási lehetõségei. >gi|39578743|emb|CAE57166.1| Hypothetical protein CBG25105 [Caenorhabditis briggsae] MTIASKERRCARLLTQSDATSYFEKYKRVRSTDMKVQQCQSVAFNCDGTKLVCGAFDKKVSIANVDGGRLRFSWVGSSHT

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A publikus adatbázisból hiányzó fehérjék azonosítási lehetõségei

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A publikus adatbázisból hiányzó fehérjék azonosítási lehetõségei >gi|39578743|emb|CAE57166.1| Hypothetical protein CBG25105 [Caenorhabditis briggsae] MTIASKERRCARLLTQSDATSYFEKYKRVRSTDMKVQQCQSVAFNCDGTKLVCGAFDKKVSIANVDGGRLRFSWVGSSHT SSVEQVACSEKQPNMFASASSDRNVCIWDARQSKPTHRITNKVGNFFVSWSPCDEYLIFLDKDNRVNTVDVRNYKVVN >gi|39578744|emb|CAE57164.1| Hypothetical protein CBG25098 [Caenorhabditis briggsae] MVENWDTSYAQVEEALLKSWPKNKESCAQLLIGYFDYYSRFDFRNFVVQCRREMTLSKMEKEWPRPICVEDPFDLNHNLS SGVTKKMFVFIMKVFINSRAVFMSEKPKMTDHNHAGFHLMYRKVLLDKCHQGSAPSDRQCHSCHRIGHFYESCPFRMQGK DNRRRYTSNSTNNSYRSTTGSIITGRRQRILMVLEIDWAIIKG >gi|39578745|emb|CAE57162.1| Hypothetical protein CBG25096 [Caenorhabditis briggsae] NVLHVIPMFPMASFIGYKAHFCYGDQLKKISESSAHILEESVDSLVPLPPTLDDVRHRSKQLKEEESLANF >gi|39578747|emb|CAE57160.1| Hypothetical protein CBG25094 [Caenorhabditis briggsae] MNGFRKLDHLNSASLTLKHLLNNSTVHQSPQSAIAEFVDNSYDANAKNCSIEVYDTPNNERIEILDDGDGMTRSEALNIV KFGFSNKVDNAIGRYGMGLKSGGLYIGRDILLLTKKDDEETAVFISHSFLRAENTDEKVYIPSPSWKYGEAHVPTIEDAE RFDDECGIINQYMSVEGYESFEQLFDKIPGEHGTLIIISKLQRDPRGELEINITGDKWDIQDIGDNLPPHKLSLRKYLEI LYLNPKMAITLRGKDVYPRNVVDNWMARYTVEYNGDMSTESLNKTIHEIQAQKAQ >gi|39578748|emb|CAE57159.1| Hypothetical protein CBG25093 [Caenorhabditis briggsae] PKNMIPMENKNLYPISDEDEIDVLNSDDEEMLPEGSFHHQPDEIYMVQEDGEGYYDDDDEDDPLQEYQYVEEDGVL >gi|39578749|emb|CAE57158.1| Hypothetical protein CBG25092 [Caenorhabditis briggsae] MSIINLLEPFQYNFRTQIFANPNKFIRAVVLYNKSQIFESFQSSGHDTFGGETAISRMFLCPPDSKTNLLTARRSFRNYH HLSSSLCSDCRFNQKRQRRLAKKEWEKRRISTECLMKFGKEKREQLRNQSILTKSRIEAADQSLFRANEMSSNGDSFGVF QNIEGVGDVENDDDDDGFF >gi|39578750|emb|CAE57157.1| Hypothetical protein CBG25091 [Caenorhabditis briggsae] PTPSSTITSVPTTVSTFTYEPPPASTFTAEPTPSSTITSVQTTVSTLTNEPPPASTFTAEPTSSSTITSEPPPSSTITSE PTPSLTITSEPPPSLTITSEPPTPSTITSEHSPASTITSEPPPSSTIISEPPPSSTITSEPHPSSTITPVPPPASTIASE PTPSSTITSVPTTVSTFTTEPPPSSTFTAEPTPSSTITSEPTPSLTITSEPPPSSTITSEPPPSSTITSEPTPSSTIPSE PTPSLTITSEPPPSLTITSEPPPSLTITSEPPPSSTITSEPLPASTFTDEPTPSLPILSEPLPSSTITSDASPLQLSPLN HLHLQLSPLYLPQFQLSPLNLLRRQAFTAEPTPSLTITSEPPPSLTITSEPPPPSTITSEHSPASTITSETPPSSTITSE PPPSSTIISEPPPSSTITSEPTPSLTITSEPTPSLTITSEPPPSLTITSEPPPSSTITSEPLPASTITSVPTTVSTFTSE PPPASTFTAEPTPSSTITSELPPSSTITSEPTPSLTITSEPTPSLPITSEPSPSLTITSEPPPSLFCRHIALVRPLETR >gi|39578751|emb|CAE57156.1| Hypothetical protein CBG25090 [Caenorhabditis briggsae] MLENLLKENLLKENLLKENLLKENLLKEENLLKENLHEENLPKENLPKENLPKENFPKENLPKENLPKENLPKENLPKEN FPKEYFPKENLHKENVRKENLPKENLPKENLPKEENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKE NLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKE NLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKKNLPKEENLPKENLPKENLPK ENLPKENLPKENLPKEENLPKENLPKENFPKENLPKEENLPKEENLPKEENLPKEENLPKEENLPKENLPKENLPKKIFL KKKIFLKKKIFLKKIFLKKIFLKKIFLKKIFLKNLPKEENLPKEENLPKEENLPKEENLPKDHLPKDHL >gi|39578752|emb|CAE57154.1| Hypothetical protein CBG25088 [Caenorhabditis briggsae] KWIQWYLLRMLLQLKMGQRTVDILHQKGISIRILSKKNTSKIESIRYEYNVGYFDKQVQLMNWIIQMFRFSGEKIVVAIQ RNSKERFLFRNRLRVTLWKRNAGGRRDYVPIHDASIPYPAHWPTDLIHFINAMLKFDKEKRLVGLEAIKKHAYTERIDFK SVFERKPAPVFIPCKEGLNCDPMYELEERILVSTPIHRRRTNHNNSSGRSSSEPQNAALVEVSKAFIDFSRHNMKIEPNG VCRSN >gi|39578754|emb|CAE57152.1| Hypothetical protein CBG25086 [Caenorhabditis briggsae] MEPKEPELTQEEIDHIAWIEKIAEQSSFEQMTAPPTRPPVPIKMATVDEPVVVTEAHEDEDRSSATSGADFQQSFDHEVT YERSSPLLEPDEVPVMEPKEPELTQEEIDHIAWIEKIAEQSSFEQMNAPPTRPPVPIKMATVDEPVVVTEAHEDEDRSSV TSGADFQQSFDHEVTYERSSPLLEPVEEPVMEPKEPELTQEEIDHIAWIQKIAEQSSFEQTTAPPTRPPVPIRMATVDEP NIVTEAHEDEDRSSATSGADFQQSFDHEVTYERSSPLLEPVEIPVKEPKEPELTQEEIDHIAWIEKIAEQSSFEQMTAPP TRPPVPIRMATVDEPNIVTEAHEDEHRSSATSGADFQQSFDHEVTYERSSPLLEPVEEPVMEPKEPQLPQEEIVHIAWIE KIAAQSSFEQMTAPPTRPPVPIRMAAVEEPNIVTEAHEDEDRSSATSGADFQQSFDHEVTYERSSPLLEPVEEPVMEPKE PELTQEEIDHIAWIEKIDEQSSFEQMTAPRTRPPVPIKMTTVDEPVVVTEAHEDEDRSSATSGADFQQSFDHEVTYERSS PLLEPVEIPVIEPKEPELTQEEIDHIAWIEKIAEQSSFEQMTAPPTRPPVPIRMAT >gi|39578755|emb|CAE57151.1| Hypothetical protein CBG25085 [Caenorhabditis briggsae] MTDGEESFKYYSKMKYLEDTQVLRCLVHRRNNLTFIQSKKNISQSDLNKIFGRQIGDEVENGIFNVMSSEAMEAEIVKYE KKEFCKSILTGKSELIRYANFKKTGILSAQSQVKALRKHGFRTFSLSHVFEMPKTVWRSVPKQMKIGEVFNKNVIYHKSG QYLVEKDDKSFFVVKTTETNIECKDPKCASKPAITCSHVFAVLSTLPTKKCQEVLNIREKFLISLSRSLQIKSINDGKDA KRYPNRKYSKRSHNNGGRTVSEFKKVNRNVSGESESGDDERKADGNISSDEENDSDCDVNSLNSKPNVFDESGDIVEDEI SCNSNDEHVNLVLDPSVDHLNSHDSCMDLSDDPRKYDSDSDEENDIFGYQNVNPVPKPSNRVSNDHSSQIDLRDVLGEKG SRKPESDSEEVTDVSTGQHVKPMSLKEKFPIGYMHRLSDDSDDSET >gi|39578756|emb|CAE57150.1| Hypothetical protein CBG25084 [Caenorhabditis briggsae] MRFFFLLHILLMLHHFISHLLIQRAACGAHIADQVLEIMPFFDQHTELLSIHSVGPWRISFLISSVFSFFFFSAAFIFST FLKVASPPEATADQKSRLDMLVQKVLNVGIDSKAQIQERTKEKVGKVMKETEEIKGKFMDIKKFTLADKRGKPIKEELEM EKKKRQMLLDEIKRLGEAKEEMSNDDDEPFENLMKEIDKLFEEADENRKLQKEADKEIATAATRQDAVFNEEMEEIEKFL SDIRNADKLQL >gi|39578757|emb|CAE57149.1| Hypothetical protein CBG25082 [Caenorhabditis briggsae] EFQNFRKTHKNSEFRNFRNLETQGSEKSEFQISGFRFQILYFRFQISDFRFQISDFRFQISDFRFQISDFRFQISDFRFQ ISDQISDFGEENVEASFYLSLKIFSEFQISGFQNFRKTHKNSEFRNFRNLETQGSEKFQISDFIFQISDFRFQISDFRFQ ISDFRFQISDFRFQISDFRFQISDFRFQISDFRFQISDQISDFGEENVEASFYLSLK >gi|39578758|emb|CAE57148.1| Hypothetical protein CBG25081 [Caenorhabditis briggsae] MYSWTRSRYWLTRSMWRWIQSRYSWWTRSMYSWTRSRWRWIQSRYSWTRSRYSLTRSMWRWIQSRYLWTRSRYSWTRSMW RWIQSMYSWTRSRWRWIQSRYSWTRSRYSLTRSKWRWIQSRYSWTRSRYSWTRSMWRWIQSRYSWTRSRYSWTRSMWRWI QLRYSWTRSRYSLTRSRWRWIQSMYSWTRSRWRWIQSMYSWTQSRWRWIQSRYSWTRSRYSLTRSMWRWIQSRYSWTRSR YWLTRSMCRWIQSRYLWTRSRYSWTRSRWRWIQSRYSWTRSRWRWIQSRYSWTRSRYSWTRSKCRWIQSRYSWTRSRYSW TRSMWRWIQLRYSWTRSRYSWTRSMWRWIQSRYLWTRSRYSWTRSMWRWIQSMYSWTRSRWRWIQSRYSWTRSRYSLTRS KWRWIQSRYSWTRS >gi|39578759|emb|CAE56373.1| Hypothetical protein CBG24048 [Caenorhabditis briggsae] GLTEYVTVMACIVVDAEPIFGAIYRPFFNETIFGLQGFGVVTSDGKKISPVDEGKTDKKVVVSRSHAGKVKEIVEKVYGD KMTIEAAGGSGYKTLRLVNGTAGKGNKYIF >gi|39578760|emb|CAE57147.1| Hypothetical protein CBG25080 [Caenorhabditis briggsae] TKPNPQNMLIGKQKKAKAEGYDTNSLAIHSKVNASDFIKSSGYLDILGEANVILLDDPKWKNHEKTTEEVVEYMKDVKVL GPRELRVLLRWRKSMLETLEAERKAVEGEAQDVEIEEDLTEEQIEDRAMAEIDELIAKASEDEKAALKKKKKKMLKAKAR VLKRRELKMIIDGDEGPQAEDQEVFQLKKIRKAKELAEITKETQAPDFENVEGKGAGWRGWGGREWLYLEISLFSAVF >gi|39578761|emb|CAE57145.1| Hypothetical protein CBG25077 [Caenorhabditis briggsae] MWAEPHHRISELFQNFRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNL RISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISEFQNL RISESQNLRISEFQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISEFQNF >gi|39578762|emb|CAE56137.1| Hypothetical protein CBG23749 [Caenorhabditis briggsae] MPNRERYVKKFTVEQKRKMDEVFVASRNPEKGKIAALVAELELTTTQVRQYFRKQRFKNPIPEEIPRFLSEEQVEHGIVD GETFKRERNSEKRNQFEDAMEKFFEERQFLEEPDETLELESGGSRKRISDWLDKKRYDTLKSFLEKEISVLPNEMATFEK LSEKYCLDTTTHPDVILYIEMKED >gi|39578763|emb|CAE57144.1| Hypothetical protein CBG25076 [Caenorhabditis briggsae] GNFHNYVCSVNGTSPDPRYKRFINFSKKLRNCGIQVTMAQMFMFQKDENSFLDYNKERSPYFKFSEICITDWEKFWKKLL QFSCSLAGYSSSHLAFYSHDLARKVLKI >gi|39578764|emb|CAE57143.1| Hypothetical protein CBG25074 [Caenorhabditis briggsae] NGGYSHRSTLSRDLPSEETITSITPFDEEKLSDDSIDQSAQEISHSSKTDDMSDEKMSGVSISPIDPDESIQLTLPNEQE ASRSSITEGIAEEMLPVDPIGTVAQGESVPSFPPTSFSDTIAQLRLYFNQELSNEGAMPAVSADRAIHSAKRTSSVPIRQ YSSDTVDENELAVKKPRFFSPQRQVLKPLNTSYKLAMSPEAPSSEHPNSLVEPLVVLQKSPEEPWAELLNRPKTQTLPEA LQQLRPLKRYGKQVTLKEYLQIQFKGQLLRTIDWNRVDDLKLFHPKTALFQSTNHPILLLAGKSFNRALLVEGNHRTAAL SECHAQKELSDDDLKSTVPLTFFQIPKPEFNNMWMAANELGRQVQAQARFDWKNASSRTREAVFSEFKKEAYRTKGKFSK FESSIL >gi|39578765|emb|CAE57142.1| Hypothetical protein CBG25073 [Caenorhabditis briggsae] MVLYQMTLFFSVQMLFAIFLEYMPKTYCTDDDYCYKMKNKCLTDFIREDDSQICPYNSTDFKQCVLDAKRVDFRSAQFDY QQDCTGLKHFSSSTATFIGTLLGNLVLIYLSDTIGRRPVFIFSIALGVPALILSAAINGVMNFYIFRFIVGFAVAGTLTV GWTYSSEMITPSRRFRLRTFPNWA >gi|39578767|emb|CAE57140.1| Hypothetical protein CBG25071 [Caenorhabditis briggsae] MAFIFSTGYQMAFMQYGMTTIITLNRLTVLLNFTFFEPIWKKYSWLLVIFLIFLPFLSTKVALNYDSKFSYQNSTDYFLL TSGMPVWEIYSILIPFMIVTIVTSLAANLSSFVFVKHRKLVMAYKA >gi|39578768|emb|CAE57141.1| Hypothetical protein CBG25072 [Caenorhabditis briggsae] ITSITLIIQVIGNFLSIGMLTFEHSDYLPYLSIIRPFVSDGLTLVQPWLLYFFSSPMRKKLQQMFKRSSPATVATVVFKS RSV >gi|39578769|emb|CAE57139.1| Hypothetical protein CBG25067 [Caenorhabditis briggsae] MYGLGFVVNSAKLANARSLWFNVGALNHLHRTWSLGQGVVFSRAVLLSCDLLRLSFD >gi|39578770|emb|CAE56385.1| Hypothetical protein CBG24068 [Caenorhabditis briggsae] MKKITESGIRNPEISGFKNGSYPRTQVIATMVIPGNMPSAFPFFLTFKLVSSCSTSSFFHKSLGNLNFRSKSVTMAKGNI FVRFKNYIVETISDTYHHIRELRAEWNRQQGRG >gi|39578771|emb|CAE57138.1| Hypothetical protein CBG25066 [Caenorhabditis briggsae] SPFKDEAVFRFSNWFKKLGDEDKDKEAEEKEKELAEIAETRFLENKQKFRSLLIERKFSAEEWTLMKQFLDYPELAAIVV KMRRSQGVPGLRQNRRRCLTCEESLAGMPLTAHKTENCSIPYESRILVSKRLFGQRKCYGCGRMKEDGNCNCKSVPCRRC WRWNVPESFPHLQVMGTCQMVWKIRQETGHVPTEHEISDYTKRNDKIASIIQHKLEKFVAETERELVDAGRASNQKTEAE LWWVADKYRTLAFSIPWHGAKGYFRKISKLFESPETI >gi|39578772|emb|CAE56541.1| Hypothetical protein CBG24269 [Caenorhabditis briggsae] MIEAVDRILVVDASIVTDTSIQKENLISSSLNQHGEINFVIETCDQYLHLHKSYIYLEVDLSKLDDTALAETDEVSLTNN APMFLFDRATYSMDGVQVENIWILVELV >gi|39578773|emb|CAE57137.1| Hypothetical protein CBG25062 [Caenorhabditis briggsae] VLQTDESGNYIYPAVGPDGQVLPTDATGKTVYPVRGPDGTPLPTDASGAVIGPDGEPIPTDASGKPLSQDGSPLPTDNQG NYVLVPTGEDVTKYLPTDESGNVIYPITRPDGTPLGTDASGNFVTDDGTVVEKDDEGKPVGPDGAVLPTDASGNYIYPVV GPDGQVLPTDATGKTVYPVRGPDGTPLPTDGSGAVIGPDGEPIPTDPSGKPLSKDGSPLPTDASGNYILVPAEEETTKTL PTDSEGNVIYPITNPDGTPLGTDSSGAFVTQDGTVIEKNEDGKPVGPDGQFVYSVIADIFFPYLQNQGPVLQISKKN >gi|39578774|emb|CAE57136.1| Hypothetical protein CBG25061 [Caenorhabditis briggsae] MGMKWTGLTFEIAAEEMRRLKSTPSEREQMKLYGLYKQALHGDIPNEDVYPVSQKEKQSLEPEKSSALAERVYLTN >gi|39578775|emb|CAE56809.1| Hypothetical protein CBG24622 [Caenorhabditis briggsae] SSFFGMLVYCVRVCMYVNDTEKEGEREELSMSYNQKRYTPKRRRNEIDDMMNAGQMAVGSSGTIAMGTGVPQKKDDMDAL STLERDVAKRTQHAQQILLRQRANQSTPRSVTPPPQPQEFKTYVISAPQQQHQQHVGNNGHQQVLHGGHMQQQQQIQVSH QEIMSEKAKFGSANVARVSLRLHWPAKYLADHFFV NO MATCH! Csorba Attila esettanulmánya az adatbázisokkal

  2. A publikus adatbázisból hiányzó fehérjék azonosítási lehetõségei >gi|39578743|emb|CAE57166.1| Hypothetical protein CBG25105 [Caenorhabditis briggsae] MTIASKERRCARLLTQSDATSYFEKYKRVRSTDMKVQQCQSVAFNCDGTKLVCGAFDKKVSIANVDGGRLRFSWVGSSHT SSVEQVACSEKQPNMFASASSDRNVCIWDARQSKPTHRITNKVGNFFVSWSPCDEYLIFLDKDNRVNTVDVRNYKVVN >gi|39578744|emb|CAE57164.1| Hypothetical protein CBG25098 [Caenorhabditis briggsae] MVENWDTSYAQVEEALLKSWPKNKESCAQLLIGYFDYYSRFDFRNFVVQCRREMTLSKMEKEWPRPICVEDPFDLNHNLS SGVTKKMFVFIMKVFINSRAVFMSEKPKMTDHNHAGFHLMYRKVLLDKCHQGSAPSDRQCHSCHRIGHFYESCPFRMQGK DNRRRYTSNSTNNSYRSTTGSIITGRRQRILMVLEIDWAIIKG >gi|39578745|emb|CAE57162.1| Hypothetical protein CBG25096 [Caenorhabditis briggsae] NVLHVIPMFPMASFIGYKAHFCYGDQLKKISESSAHILEESVDSLVPLPPTLDDVRHRSKQLKEEESLANF >gi|39578747|emb|CAE57160.1| Hypothetical protein CBG25094 [Caenorhabditis briggsae] MNGFRKLDHLNSASLTLKHLLNNSTVHQSPQSAIAEFVDNSYDANAKNCSIEVYDTPNNERIEILDDGDGMTRSEALNIV KFGFSNKVDNAIGRYGMGLKSGGLYIGRDILLLTKKDDEETAVFISHSFLRAENTDEKVYIPSPSWKYGEAHVPTIEDAE RFDDECGIINQYMSVEGYESFEQLFDKIPGEHGTLIIISKLQRDPRGELEINITGDKWDIQDIGDNLPPHKLSLRKYLEI LYLNPKMAITLRGKDVYPRNVVDNWMARYTVEYNGDMSTESLNKTIHEIQAQKAQ >gi|39578748|emb|CAE57159.1| Hypothetical protein CBG25093 [Caenorhabditis briggsae] PKNMIPMENKNLYPISDEDEIDVLNSDDEEMLPEGSFHHQPDEIYMVQEDGEGYYDDDDEDDPLQEYQYVEEDGVL >gi|39578749|emb|CAE57158.1| Hypothetical protein CBG25092 [Caenorhabditis briggsae] MSIINLLEPFQYNFRTQIFANPNKFIRAVVLYNKSQIFESFQSSGHDTFGGETAISRMFLCPPDSKTNLLTARRSFRNYH HLSSSLCSDCRFNQKRQRRLAKKEWEKRRISTECLMKFGKEKREQLRNQSILTKSRIEAADQSLFRANEMSSNGDSFGVF QNIEGVGDVENDDDDDGFF >gi|39578750|emb|CAE57157.1| Hypothetical protein CBG25091 [Caenorhabditis briggsae] PTPSSTITSVPTTVSTFTYEPPPASTFTAEPTPSSTITSVQTTVSTLTNEPPPASTFTAEPTSSSTITSEPPPSSTITSE PTPSLTITSEPPPSLTITSEPPTPSTITSEHSPASTITSEPPPSSTIISEPPPSSTITSEPHPSSTITPVPPPASTIASE PTPSSTITSVPTTVSTFTTEPPPSSTFTAEPTPSSTITSEPTPSLTITSEPPPSSTITSEPPPSSTITSEPTPSSTIPSE PTPSLTITSEPPPSLTITSEPPPSLTITSEPPPSSTITSEPLPASTFTDEPTPSLPILSEPLPSSTITSDASPLQLSPLN HLHLQLSPLYLPQFQLSPLNLLRRQAFTAEPTPSLTITSEPPPSLTITSEPPPPSTITSEHSPASTITSETPPSSTITSE PPPSSTIISEPPPSSTITSEPTPSLTITSEPTPSLTITSEPPPSLTITSEPPPSSTITSEPLPASTITSVPTTVSTFTSE PPPASTFTAEPTPSSTITSELPPSSTITSEPTPSLTITSEPTPSLPITSEPSPSLTITSEPPPSLFCRHIALVRPLETR >gi|39578751|emb|CAE57156.1| Hypothetical protein CBG25090 [Caenorhabditis briggsae] MLENLLKENLLKENLLKENLLKENLLKEENLLKENLHEENLPKENLPKENLPKENFPKENLPKENLPKENLPKENLPKEN FPKEYFPKENLHKENVRKENLPKENLPKENLPKEENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKE NLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKE NLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKENLPKKNLPKEENLPKENLPKENLPK ENLPKENLPKENLPKEENLPKENLPKENFPKENLPKEENLPKEENLPKEENLPKEENLPKEENLPKENLPKENLPKKIFL KKKIFLKKKIFLKKIFLKKIFLKKIFLKKIFLKNLPKEENLPKEENLPKEENLPKEENLPKDHLPKDHL >gi|39578752|emb|CAE57154.1| Hypothetical protein CBG25088 [Caenorhabditis briggsae] KWIQWYLLRMLLQLKMGQRTVDILHQKGISIRILSKKNTSKIESIRYEYNVGYFDKQVQLMNWIIQMFRFSGEKIVVAIQ RNSKERFLFRNRLRVTLWKRNAGGRRDYVPIHDASIPYPAHWPTDLIHFINAMLKFDKEKRLVGLEAIKKHAYTERIDFK SVFERKPAPVFIPCKEGLNCDPMYELEERILVSTPIHRRRTNHNNSSGRSSSEPQNAALVEVSKAFIDFSRHNMKIEPNG VCRSN >gi|39578754|emb|CAE57152.1| Hypothetical protein CBG25086 [Caenorhabditis briggsae] MEPKEPELTQEEIDHIAWIEKIAEQSSFEQMTAPPTRPPVPIKMATVDEPVVVTEAHEDEDRSSATSGADFQQSFDHEVT YERSSPLLEPDEVPVMEPKEPELTQEEIDHIAWIEKIAEQSSFEQMNAPPTRPPVPIKMATVDEPVVVTEAHEDEDRSSV TSGADFQQSFDHEVTYERSSPLLEPVEEPVMEPKEPELTQEEIDHIAWIQKIAEQSSFEQTTAPPTRPPVPIRMATVDEP NIVTEAHEDEDRSSATSGADFQQSFDHEVTYERSSPLLEPVEIPVKEPKEPELTQEEIDHIAWIEKIAEQSSFEQMTAPP TRPPVPIRMATVDEPNIVTEAHEDEHRSSATSGADFQQSFDHEVTYERSSPLLEPVEEPVMEPKEPQLPQEEIVHIAWIE KIAAQSSFEQMTAPPTRPPVPIRMAAVEEPNIVTEAHEDEDRSSATSGADFQQSFDHEVTYERSSPLLEPVEEPVMEPKE PELTQEEIDHIAWIEKIDEQSSFEQMTAPRTRPPVPIKMTTVDEPVVVTEAHEDEDRSSATSGADFQQSFDHEVTYERSS PLLEPVEIPVIEPKEPELTQEEIDHIAWIEKIAEQSSFEQMTAPPTRPPVPIRMAT >gi|39578755|emb|CAE57151.1| Hypothetical protein CBG25085 [Caenorhabditis briggsae] MTDGEESFKYYSKMKYLEDTQVLRCLVHRRNNLTFIQSKKNISQSDLNKIFGRQIGDEVENGIFNVMSSEAMEAEIVKYE KKEFCKSILTGKSELIRYANFKKTGILSAQSQVKALRKHGFRTFSLSHVFEMPKTVWRSVPKQMKIGEVFNKNVIYHKSG QYLVEKDDKSFFVVKTTETNIECKDPKCASKPAITCSHVFAVLSTLPTKKCQEVLNIREKFLISLSRSLQIKSINDGKDA KRYPNRKYSKRSHNNGGRTVSEFKKVNRNVSGESESGDDERKADGNISSDEENDSDCDVNSLNSKPNVFDESGDIVEDEI SCNSNDEHVNLVLDPSVDHLNSHDSCMDLSDDPRKYDSDSDEENDIFGYQNVNPVPKPSNRVSNDHSSQIDLRDVLGEKG SRKPESDSEEVTDVSTGQHVKPMSLKEKFPIGYMHRLSDDSDDSET >gi|39578756|emb|CAE57150.1| Hypothetical protein CBG25084 [Caenorhabditis briggsae] MRFFFLLHILLMLHHFISHLLIQRAACGAHIADQVLEIMPFFDQHTELLSIHSVGPWRISFLISSVFSFFFFSAAFIFST FLKVASPPEATADQKSRLDMLVQKVLNVGIDSKAQIQERTKEKVGKVMKETEEIKGKFMDIKKFTLADKRGKPIKEELEM EKKKRQMLLDEIKRLGEAKEEMSNDDDEPFENLMKEIDKLFEEADENRKLQKEADKEIATAATRQDAVFNEEMEEIEKFL SDIRNADKLQL >gi|39578757|emb|CAE57149.1| Hypothetical protein CBG25082 [Caenorhabditis briggsae] EFQNFRKTHKNSEFRNFRNLETQGSEKSEFQISGFRFQILYFRFQISDFRFQISDFRFQISDFRFQISDFRFQISDFRFQ ISDQISDFGEENVEASFYLSLKIFSEFQISGFQNFRKTHKNSEFRNFRNLETQGSEKFQISDFIFQISDFRFQISDFRFQ ISDFRFQISDFRFQISDFRFQISDFRFQISDFRFQISDQISDFGEENVEASFYLSLK >gi|39578758|emb|CAE57148.1| Hypothetical protein CBG25081 [Caenorhabditis briggsae] MYSWTRSRYWLTRSMWRWIQSRYSWWTRSMYSWTRSRWRWIQSRYSWTRSRYSLTRSMWRWIQSRYLWTRSRYSWTRSMW RWIQSMYSWTRSRWRWIQSRYSWTRSRYSLTRSKWRWIQSRYSWTRSRYSWTRSMWRWIQSRYSWTRSRYSWTRSMWRWI QLRYSWTRSRYSLTRSRWRWIQSMYSWTRSRWRWIQSMYSWTQSRWRWIQSRYSWTRSRYSLTRSMWRWIQSRYSWTRSR YWLTRSMCRWIQSRYLWTRSRYSWTRSRWRWIQSRYSWTRSRWRWIQSRYSWTRSRYSWTRSKCRWIQSRYSWTRSRYSW TRSMWRWIQLRYSWTRSRYSWTRSMWRWIQSRYLWTRSRYSWTRSMWRWIQSMYSWTRSRWRWIQSRYSWTRSRYSLTRS KWRWIQSRYSWTRS >gi|39578759|emb|CAE56373.1| Hypothetical protein CBG24048 [Caenorhabditis briggsae] GLTEYVTVMACIVVDAEPIFGAIYRPFFNETIFGLQGFGVVTSDGKKISPVDEGKTDKKVVVSRSHAGKVKEIVEKVYGD KMTIEAAGGSGYKTLRLVNGTAGKGNKYIF >gi|39578760|emb|CAE57147.1| Hypothetical protein CBG25080 [Caenorhabditis briggsae] TKPNPQNMLIGKQKKAKAEGYDTNSLAIHSKVNASDFIKSSGYLDILGEANVILLDDPKWKNHEKTTEEVVEYMKDVKVL GPRELRVLLRWRKSMLETLEAERKAVEGEAQDVEIEEDLTEEQIEDRAMAEIDELIAKASEDEKAALKKKKKKMLKAKAR VLKRRELKMIIDGDEGPQAEDQEVFQLKKIRKAKELAEITKETQAPDFENVEGKGAGWRGWGGREWLYLEISLFSAVF >gi|39578761|emb|CAE57145.1| Hypothetical protein CBG25077 [Caenorhabditis briggsae] MWAEPHHRISELFQNFRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNL RISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISEFQNL RISESQNLRISEFQNLRISESQNLRISESQNLRISESQNLRISESQNLRISESQNLRISEFQNF >gi|39578762|emb|CAE56137.1| Hypothetical protein CBG23749 [Caenorhabditis briggsae] MPNRERYVKKFTVEQKRKMDEVFVASRNPEKGKIAALVAELELTTTQVRQYFRKQRFKNPIPEEIPRFLSEEQVEHGIVD GETFKRERNSEKRNQFEDAMEKFFEERQFLEEPDETLELESGGSRKRISDWLDKKRYDTLKSFLEKEISVLPNEMATFEK LSEKYCLDTTTHPDVILYIEMKED >gi|39578763|emb|CAE57144.1| Hypothetical protein CBG25076 [Caenorhabditis briggsae] GNFHNYVCSVNGTSPDPRYKRFINFSKKLRNCGIQVTMAQMFMFQKDENSFLDYNKERSPYFKFSEICITDWEKFWKKLL QFSCSLAGYSSSHLAFYSHDLARKVLKI >gi|39578764|emb|CAE57143.1| Hypothetical protein CBG25074 [Caenorhabditis briggsae] NGGYSHRSTLSRDLPSEETITSITPFDEEKLSDDSIDQSAQEISHSSKTDDMSDEKMSGVSISPIDPDESIQLTLPNEQE ASRSSITEGIAEEMLPVDPIGTVAQGESVPSFPPTSFSDTIAQLRLYFNQELSNEGAMPAVSADRAIHSAKRTSSVPIRQ YSSDTVDENELAVKKPRFFSPQRQVLKPLNTSYKLAMSPEAPSSEHPNSLVEPLVVLQKSPEEPWAELLNRPKTQTLPEA LQQLRPLKRYGKQVTLKEYLQIQFKGQLLRTIDWNRVDDLKLFHPKTALFQSTNHPILLLAGKSFNRALLVEGNHRTAAL SECHAQKELSDDDLKSTVPLTFFQIPKPEFNNMWMAANELGRQVQAQARFDWKNASSRTREAVFSEFKKEAYRTKGKFSK FESSIL >gi|39578765|emb|CAE57142.1| Hypothetical protein CBG25073 [Caenorhabditis briggsae] MVLYQMTLFFSVQMLFAIFLEYMPKTYCTDDDYCYKMKNKCLTDFIREDDSQICPYNSTDFKQCVLDAKRVDFRSAQFDY QQDCTGLKHFSSSTATFIGTLLGNLVLIYLSDTIGRRPVFIFSIALGVPALILSAAINGVMNFYIFRFIVGFAVAGTLTV GWTYSSEMITPSRRFRLRTFPNWA >gi|39578767|emb|CAE57140.1| Hypothetical protein CBG25071 [Caenorhabditis briggsae] MAFIFSTGYQMAFMQYGMTTIITLNRLTVLLNFTFFEPIWKKYSWLLVIFLIFLPFLSTKVALNYDSKFSYQNSTDYFLL TSGMPVWEIYSILIPFMIVTIVTSLAANLSSFVFVKHRKLVMAYKA >gi|39578768|emb|CAE57141.1| Hypothetical protein CBG25072 [Caenorhabditis briggsae] ITSITLIIQVIGNFLSIGMLTFEHSDYLPYLSIIRPFVSDGLTLVQPWLLYFFSSPMRKKLQQMFKRSSPATVATVVFKS RSV >gi|39578769|emb|CAE57139.1| Hypothetical protein CBG25067 [Caenorhabditis briggsae] MYGLGFVVNSAKLANARSLWFNVGALNHLHRTWSLGQGVVFSRAVLLSCDLLRLSFD >gi|39578770|emb|CAE56385.1| Hypothetical protein CBG24068 [Caenorhabditis briggsae] MKKITESGIRNPEISGFKNGSYPRTQVIATMVIPGNMPSAFPFFLTFKLVSSCSTSSFFHKSLGNLNFRSKSVTMAKGNI FVRFKNYIVETISDTYHHIRELRAEWNRQQGRG >gi|39578771|emb|CAE57138.1| Hypothetical protein CBG25066 [Caenorhabditis briggsae] SPFKDEAVFRFSNWFKKLGDEDKDKEAEEKEKELAEIAETRFLENKQKFRSLLIERKFSAEEWTLMKQFLDYPELAAIVV KMRRSQGVPGLRQNRRRCLTCEESLAGMPLTAHKTENCSIPYESRILVSKRLFGQRKCYGCGRMKEDGNCNCKSVPCRRC WRWNVPESFPHLQVMGTCQMVWKIRQETGHVPTEHEISDYTKRNDKIASIIQHKLEKFVAETERELVDAGRASNQKTEAE LWWVADKYRTLAFSIPWHGAKGYFRKISKLFESPETI >gi|39578772|emb|CAE56541.1| Hypothetical protein CBG24269 [Caenorhabditis briggsae] MIEAVDRILVVDASIVTDTSIQKENLISSSLNQHGEINFVIETCDQYLHLHKSYIYLEVDLSKLDDTALAETDEVSLTNN APMFLFDRATYSMDGVQVENIWILVELV >gi|39578773|emb|CAE57137.1| Hypothetical protein CBG25062 [Caenorhabditis briggsae] VLQTDESGNYIYPAVGPDGQVLPTDATGKTVYPVRGPDGTPLPTDASGAVIGPDGEPIPTDASGKPLSQDGSPLPTDNQG NYVLVPTGEDVTKYLPTDESGNVIYPITRPDGTPLGTDASGNFVTDDGTVVEKDDEGKPVGPDGAVLPTDASGNYIYPVV GPDGQVLPTDATGKTVYPVRGPDGTPLPTDGSGAVIGPDGEPIPTDPSGKPLSKDGSPLPTDASGNYILVPAEEETTKTL PTDSEGNVIYPITNPDGTPLGTDSSGAFVTQDGTVIEKNEDGKPVGPDGQFVYSVIADIFFPYLQNQGPVLQISKKN >gi|39578774|emb|CAE57136.1| Hypothetical protein CBG25061 [Caenorhabditis briggsae] MGMKWTGLTFEIAAEEMRRLKSTPSEREQMKLYGLYKQALHGDIPNEDVYPVSQKEKQSLEPEKSSALAERVYLTN >gi|39578775|emb|CAE56809.1| Hypothetical protein CBG24622 [Caenorhabditis briggsae] SSFFGMLVYCVRVCMYVNDTEKEGEREELSMSYNQKRYTPKRRRNEIDDMMNAGQMAVGSSGTIAMGTGVPQKKDDMDAL STLERDVAKRTQHAQQILLRQRANQSTPRSVTPPPQPQEFKTYVISAPQQQHQQHVGNNGHQQVLHGGHMQQQQQIQVSH QEIMSEKAKFGSANVARVSLRLHWPAKYLADHFFV NO MATCH! Csorba Attila esettanulmánya az adatbázisokkal

  3. ZULU 20050818 1123

  4. Célpont: Capsicum annuum kitinázaktivitást mutató fehérjéi,aktivitásfestés alapján, 1D SDS-PAGE gélcsíkok,(Ca1, Ca2, Ca3, Ca4, Ca5,3220kDa) Probléma: Capsicum annuum, alias paprika A fehérjeadatbázis defektívaz alanyra nézve. Bizonyítás: Capsicum annuum vs. Oryza sativa (paprika kontra rizs) Fehérjetalálatok száma az NCBInr 2006.04.20-i adatbázisban: 675 121910 ZULU 20050818 1123

  5. Célpont: Capsicum annuum kitinázaktivitást mutató fehérjéi,aktivitásfestés alapján, 1D SDS-PAGE gélcsíkok,(Ca1, Ca2, Ca3, Ca4, Ca5, 3220kDa) Probléma: Capsicum annuum, alias paprika A fehérjeadatbázis defektívaz alanyra nézve. Bizonyítás: Capsicum annuum vs. Oryza sativa (paprika kontra rizs) Fehérjetalálatok száma az NCBInr 2006.04.20-i adatbázisban: >3.5 millió(6×10-18mol) 675 121910

  6. Felszerelés: • Bruker Reflex III, MALDI-TOFnagy felbontás (>10000), pontosság (∆m<200ppm), szimpla minták jó azonosíthatósága (PSD) • Agilent 1100 series, LC/MSD Trap, HPLC-oszlop: Zorbax 300SB-C18, 3.5µm, 150mm×75µmkomplex minták könnyű azonosítása, nagyszámú MS/MS adat Stratégia:      

  7. B-terv:    

  8. B-terv:    

  9. ? ! Mission possible

  10. 1. 2. MALDI-TOF, Ca1: Mi a különbséga szekvenciákközött? 1. gi|72534134 peroxidasePOA1 [Capsicum annuum] 2. gi|17066703 peroxidase [Capsicum annuum] PDB lekeresés:

  11. RVGFYSSTCPRAESIV QSTVRSHFQSDPTVAPGLLRMHFHDCFVQGCDASILISGSGTERTAPPNS LLRGYEVIDDAKQQIEAICPGVVSCADILALAARDSVVVTRGLTWSVPTG RRDGLVSRASDTSDLPGFTESVDSQKQKFSAKGLNTQDLVTLVGGHTIGT SACQFFSYRLYNFNSTGGPDPSIDASFLPTLRGLCPQNGDGSKRVALDTG SVNNFGTSYFSNLRNGRGILESDQKLWTDDSTKVFIQRYLGLRGFLGLRF GVEFGRSMVKMSNIEVKTGTNGEIRKVCS 1. gi|72534134 2. gi|17066703 MEYYYNYNSI NKMVSIIFIL VLAIDLTMVL GQGTRVGFYSSTCPRAESIV QSTVRSHFQS DPTVAPGLLT MHFHDCFVQG CDASILISGS GTERTAPPNS LLRGYEVIDD AKQQIEAICPGVVSCADILALAARDSVLVT KGLTWSVPTG RRDGLVSRASDTSDLPGFTESVDSQKQKFS AKGLNTQDLV TLVGGHTIGT SACQFFSYRL YNFNSTGGPD PSIDASFLPT LRGLCPQNGDGSKRVALDTG SVNNFDTSYFSNLRNGRGIL ESDQKLWTDDSTKVFIQRYL GLRGFLGLRF GVEFGRSMVK MSNIEVKTGT NGEIRKVCSA IN Ca1: Lefedettségi térkép, szekvenciák összehasonítása Akkor mostmelyik? Válasz:PSD analízis!

  12. 1. m/z: 1624,85 Ca1: PSD analízis, m/z: 1624,85 b-ionok \\\\\SHFQSDPTVAPGLLR\\ \ y-ionok MALDI-TOFalapján az erőshomológiaellenére:1. gi|72534134peroxidasePOA1[Capsicum annuum]

  13. Ca2: Ca3: Ca4: Ca5:

  14. Ca2:

  15. Ca3:

  16. Ca4:

  17. Ca5:

  18. MALDI-TOF összefoglalás: Ca1: PSD-vel igazolt paprikafehérje Ca2, Ca3, Ca4, Ca5: A lekeresések eredménye nem bíztató, PSD analízisre alkalmatlan minták • Ca2, Ca5: Gyenge spektrumok • Ca3, Ca4: Túl komplex minták B-terv: LC-MS/MS

  19. LC-MS/MS eredmények az NCBInr adatbázisából:

  20. Egy kis növényrendszertan:

  21. tBLASTn: TIGR adatbázis (genom adatbázis, 6 frame fordítás) http://www.tigr.org/

  22. Mission completed

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